Genetic control of allozyme variants in mature tissues of white spruce trees

Cheliak, W. M.; Pitel, J. A.

doi:10.1093/oxfordjournals.jhered.a109861

Cited by 69 publications

(24 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The gel and bridging buffers were prepared according to Wendel and Weeden (1989) and Creasey et al (1997). Staining protocols were obtained from Cheliak and Pitel (1984). Initially, 14 enzyme systems were trailed using six different buffer systems.…”

Section: Methodsmentioning

confidence: 99%

Patterns of morphological and genetic variability in UK populations of the shore crab, Carcinus maenas Linnaeus, 1758 (Crustacea: Decapoda: Brachyura)

Brian

Fernandes

Ladle

et al. 2006

Journal of Experimental Marine Biology and Ecology

View full text Add to dashboard Cite

Previous research has identified extensive inter-population variability in the morphology of the shore crab (Carcinus maenas L.). To determine the source of this variation (genetic or environmental), morphological and genetic data were analysed from crabs collected from eight sites around the coast of the UK. Ten morphometric traits were measured from over 800 crabs and the degree of morphological similarity among sites calculated using multivariate techniques. Allozyme electrophoresis was used to investigate patterns of genetic similarity. Extensive morphological variability was detected; eight out of the ten morphometric traits analysed were useful when discriminating between crabs from each site. Discriminant function analysis revealed that over 35% of individuals could be classified to their site of origin on the basis of their morphology. In contrast, the allozyme analysis revealed low levels of genetic variability, both within the meta-population and among the crab population at each site. Pairwise comparisons revealed a moderate correlation between the degree of morphological and genetic similarity of crabs at each site, which suggests that the observed phenotypic variability has a genetic component. However, only around 20% of the phenotypic variability detected was associated with the patterns of genetic similarity. This means that patterns of morphological variability in this species are largely determined by the local environmental conditions: local factors could have a within-generation selective influence on mean trait values or C. maenas may exhibit phenotypic plasticity.

show abstract

Section: Methodsmentioning

confidence: 99%

Patterns of morphological and genetic variability in UK populations of the shore crab, Carcinus maenas Linnaeus, 1758 (Crustacea: Decapoda: Brachyura)

Brian

Fernandes

Ladle

et al. 2006

Journal of Experimental Marine Biology and Ecology

View full text Add to dashboard Cite

show abstract

“…Leaves were cut finely, and crushed with a mortar and pestle in an extraction buffer. In preliminary trials, enzyme activity showed the best results in the Cheliak and Pitel [1] extraction buffer with some modifications. Then, enzyme extract was absorbed onto 4 mm × 10 mm wicks cut from Whatmann 3MM chromatography paper, which were stored at -70 °C until needed for analysis.…”

Section: Enzyme Extraction and Allozyme Proceduresmentioning

confidence: 99%

Lack of allozyme and ISSR variation in the Rare endemic tree species, Berchemia berchemiaefolia (Rhamnaceae) in Korea

Lee

Kim

2003

Ann. For. Sci.

View full text Add to dashboard Cite

-Rare plant species are commonly hypothesized to have little genetic variation because of genetic drift, strong and directional selection toward genetic uniformity in a limited number of environments, inbreeding depression and/or other factors. We investigated genetic variation in Berchemia berchemiafolia, a rare and endangered tree species worldwide, by examining 14 allozyme loci and 28 I-SSR amplicons in 111 individuals distributed among four populations in Korea. No allozyme and I-SSR variation were detected with the exception of one variant from one individual at Pgi-2 locus. A substantial genetic bottleneck accompanying the fluctuation of local population size caused by repeated human activities and inbreeding could account for this species' lack of genetic variation. Berchemia berchemiaefolia

show abstract

“…However, the inheritance of G2DH has been demonstrated in other conifers (Cheliak & Pitel, 1984;Shiraishi, 1988;Na'iem et al,, 1989;Suyama ci a!., 1992). All the loci, except Est-2, detected in the needles corresponded to those observed in seed megagametophytes (Wang ci al., 1996).…”

Section: Enzymes and Thefr Sta/ningsmentioning

confidence: 99%