Genetic Control and Mechanisms of Salt and Hyperosmotic Stress Resistance in Cyanobacteria

Karandashova, I. V.; Elanskaya, I.V.

doi:10.1007/s11177-006-0001-z

Cited by 21 publications

(15 citation statements)

References 105 publications

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“…Water stress inhibited cell division in Chroococcus minor, Gloeocapsa aeruginosa and Aphanothece nidulans (Agrawal and Pal 2003). Salt stress suppressed photosynthetic activity and oxygen consumption in bluegreen algal cells (Lu and Vonshak 2002;Karandashova and Elanskaya 2005;Wieland and Kuehl 2006). Vol.…”

Section: Resultsmentioning

confidence: 99%

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Vegetative survival of some wall and soil blue-green algae under stress conditions

Gupta

Agrawal

2008

Folia Microbiol

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Lyngbya major (a wall alga), survived throughout year, maximally to >80 % at atmospheric temperature (AT) of 17-36 degrees C and relative humidity (RH) 60-100 % in rainy and spring seasons, but the survival was 43-64 % in winter when AT decreased to 5 degrees C and RH was 65-98 %, and 15-23 % in summer when AT reached 48 degrees C and RH was 23-60 %. All soil algae (Lyngbya birgei, Aphanothece pallida, Gloeocapsa atrata, Oscillatoria subbrevis, O. animalis) survived >90 % in rainy season when soil moisture content (SMC) was 89-100 %. Lowering of SMC to a minimum of 55 % in spring and 39 % in winter led L. birgei, O. subbrevis and O. animalis to survive from 75, 66, and 65 %, respectively, in spring and 12, 14, and 20 % in winter, and A. pallida and G. atrata not at all in both seasons. All soil algae did not survive in summer when SMC was 12-30 %. Myxosarcina burmensis survived only in rainy and spring seasons when pond water temperature (PWT) was 19-25 degrees C and 18-26 degrees C, respectively, and not in winter and summer when PWT was 2-14 degrees C and 25-36 degrees C, respectively. L. major and A. pallida survived almost equally well under both submerged and air-exposed conditions for 15 d but less if submerged for more time than air-exposed on moist soil surface, while L. birgei, G. atrata, O. subbrevis, and O. animalis survived submergence in liquid medium better and longer than air-exposure on moist soil surface. Pond alga M. burmensis survived submergence better than air-exposure, true to its aquatic habitat. All algae survived less and died without forming any resistant cells when exposed to physical and physiological water stress (imposed by growing them on highly agarized media or in salinized liquid media), light stress (at 0, 2 and 10 micromol m(-2) s(-1) light intensity) or following UV shock (0.96-3.84 kJ/m(2)). A. pallida and G. atrata cells did not divide on 8 % agarized solid media, in > or =0.3 mol/L salinized liquid media, and in darkness. The presence of sheath over L. major and L. birgei filament cells and mucilage cover over A. pallida and G. atrata cells protect them against physical desiccation to some extent but not against UV shock.

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Section: Resultsmentioning

confidence: 99%

“…Oscillatoria acuminata developed a thin sheath when grown in 0.8 mol/L NaCl-containing BG11 (Agrawal and Singh 1999). During salt adaptation, blue-green algal cells accumulate osmoprotectors, export excessive Na + and actively absorb K + (Karandashova and Elanskaya 2005).…”

Section: Resultsmentioning

confidence: 99%

Vegetative survival of some wall and soil blue-green algae under stress conditions

Gupta

Agrawal

2008

Folia Microbiol

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“…Our last comprehensive overview was in 1996 (Joset et al, 1996). The biosynthesis of compatible solutes among cyanobacteria was briefly reviewed in the last decade Hagemann et al, 1999b;Karandashova & Elanskaya, 2005). Recently, reviews were published on ecological aspects of salt-exposed cyanobacteria (Oren, 2000(Oren, , 2007 as well as the effects of salt on the photosynthetic apparatus (Allakhverdiev & Murata, 2008).…”

Section: Introductionmentioning

confidence: 99%

Molecular biology of cyanobacterial salt acclimation

2011

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High and changing salt concentrations represent major abiotic factors limiting the growth of microorganisms. During their long evolution, cyanobacteria have adapted to aquatic habitats with various salt concentrations. High salt concentrations in the medium challenge the cell with reduced water availability and high contents of inorganic ions. The basic mechanism of salt acclimation involves the active extrusion of toxic inorganic ions and the accumulation of compatible solutes, including sucrose, trehalose, glucosylglycerol, and glycine betaine. The kinetics of these physiological processes has been exceptionally well studied in the model Synechocystis 6803, leading to the definition of five subsequent phases in reaching a new salt acclimation steady state. Recent '-omics' technologies using the advanced model Synechocystis 6803 have revealed a comprehensive picture of the dynamic process of salt acclimation involving the differential expression of hundreds of genes. However, the mechanisms involved in sensing specific salt stress signals are not well resolved. In the future, analysis of cyanobacterial salt acclimation will be directed toward defining the functions of the many unknown proteins upregulated in salt-stressed cells, identifying specific salt-sensing mechanisms, using salt-resistant strains of cyanobacteria for the production of bioenergy, and applying cyanobacterial stress genes to improve the salt tolerance of sensitive organisms.

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“…These changes directly affect the chemical composition of the cell, allowing it to maintain integrity, and the study of these changes is referred to as ''microbial phenotyping'' (Tummala et al, 2003;Sellick & Reece, 2005;Alsaker, Paredes & Papoutsakis, 2010;Nicolaou, Gaida & Papoutsakis, 2010). For example, exposure to high salt concentrations (e.g., NaCl, MgSO 4 ) results in ionic and osmotic imbalances (Markovitz & Sylvan, 1962;Lusk, Williams & Kennedy, 1968;Reed et al, 1985;Mikkat et al, 2000;Karandashova & Elanskaya, 2005;Marin et al, 2006). This leads to the accumulation of carboxylic acids (e.g.…”

Section: Introductionmentioning

confidence: 99%

Characterizing metabolic stress-induced phenotypes ofSynechocystisPCC6803 with Raman spectroscopy

Tanniche

Collakova

Denbow

et al. 2020

PeerJ

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Background During their long evolution, Synechocystis sp. PCC6803 developed a remarkable capacity to acclimate to diverse environmental conditions. In this study, Raman spectroscopy and Raman chemometrics tools (RametrixTM) were employed to investigate the phenotypic changes in response to external stressors and correlate specific Raman bands with their corresponding biomolecules determined with widely used analytical methods. Methods Synechocystis cells were grown in the presence of (i) acetate (7.5–30 mM), (ii) NaCl (50–150 mM) and (iii) limiting levels of MgSO4 (0–62.5 mM) in BG-11 media. Principal component analysis (PCA) and discriminant analysis of PCs (DAPC) were performed with the RametrixTM LITE Toolbox for MATLABⓇ. Next, validation of these models was realized via RametrixTM PRO Toolbox where prediction of accuracy, sensitivity, and specificity for an unknown Raman spectrum was calculated. These analyses were coupled with statistical tests (ANOVA and pairwise comparison) to determine statistically significant changes in the phenotypic responses. Finally, amino acid and fatty acid levels were measured with well-established analytical methods. The obtained data were correlated with previously established Raman bands assigned to these biomolecules. Results Distinguishable clusters representative of phenotypic responses were observed based on the external stimuli (i.e., acetate, NaCl, MgSO4, and controls grown on BG-11 medium) or its concentration when analyzing separately. For all these cases, RametrixTM PRO was able to predict efficiently the corresponding concentration in the culture media for an unknown Raman spectra with accuracy, sensitivity and specificity exceeding random chance. Finally, correlations (R > 0.7) were observed for all amino acids and fatty acids between well-established analytical methods and Raman bands.

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Genetic Control and Mechanisms of Salt and Hyperosmotic Stress Resistance in Cyanobacteria

Cited by 21 publications

References 105 publications

Vegetative survival of some wall and soil blue-green algae under stress conditions

Vegetative survival of some wall and soil blue-green algae under stress conditions

Molecular biology of cyanobacterial salt acclimation

Characterizing metabolic stress-induced phenotypes ofSynechocystisPCC6803 with Raman spectroscopy

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