Genetic Consequences of Interspecific Hybridization, Its Role in Speciation and Phenotypic Diversity of Plants

Rodionov, A. V.; Amosova, Аlexandra V.; Belyakov, E. A.; Zhurbenko, Peter M.; Mikhaǐlova, Yu. V.; Punina, E. O.; Shneyer, V. S.; Лоскутов, И. Г.; Муравенко, О. В.

doi:10.1134/s1022795419030141

Cited by 26 publications

(18 citation statements)

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“…Whole genome duplication by unreduced gamete production is a quite common consequence of interspecific hybridization in plants and the main mechanism to produce fertile hybrids, eventually leading to sympatric speciation ( Rodionov et al, 2019 ). However, the preferred vegetative propagation of duckweeds makes fertility not a compelling requirement for speciation.…”

Section: Discussionmentioning

confidence: 99%

Duckweed Species Genotyping and Interspecific Hybrid Discovery by Tubulin-Based Polymorphism Fingerprinting

et al. 2021

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Duckweeds (Lemnaceae) are the smallest and fastest-growing angiosperms. This feature, together with high starch production and good nutritional properties, makes them suitable for several applications, including wastewater treatment, bioenergy production, or feed and food supplement. Due to their reduced morphology and great similarity between diverse species, taxonomic identification of duckweeds is a challenging issue even for experts. Among molecular genotyping methods, DNA barcoding is the most useful tool for species identification without a need for cluster analysis. The combination of two plastid barcoding loci is now considered the gold standard for duckweed classification. However, not all species can be defined with confidence by these markers, and a fast identification method able to solve doubtful cases is missing. Here we show the potential of tubulin-based polymorphism (TBP), a molecular marker based on the intron length polymorphisms of β-tubulin loci, in the genomic profiling of the genera Spirodela, Landoltia, and Lemna. Ninety-four clones were analyzed, including at least two representatives of each species of the three genera, with a special focus on the very heterogeneous species Lemna minor. We showed that a single PCR amplification with universal primers, followed by agarose gel analysis, was able to provide distinctive fingerprinting profiles for 10 out of 15 species. Cluster analysis of capillary electrophoresis–TBP data provided good separation for the remaining species, although the relationship between L. minor and Lemna japonica was not fully resolved. However, an accurate comparison of TBP profiles provided evidence for the unexpected existence of intraspecific hybrids between Lemna turionifera and L. minor, as further confirmed by amplified fragment length polymorphism and sequence analysis of a specific β-tubulin locus. Such hybrids could possibly correspond to L. japonica, as originally suggested by E. Landolt. The discovery of interspecific hybrids opens a new perspective to understand the speciation mechanisms in the family of duckweeds.

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Section: Discussionmentioning

confidence: 99%

Duckweed Species Genotyping and Interspecific Hybrid Discovery by Tubulin-Based Polymorphism Fingerprinting

et al. 2021

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“…(x = 7) and another hypothetical ancestor of Z. trichopoda having x = 2 could be reproduced in the first generations only vegetatively, at this time there was a crossing over or conversion, or translocation with the transfer of ribotype D rDNA to the chromosomes of the bichromosomal genome. Then, gradually or saltationally, there was a loss of all Poa chromosomes and a duplication of low-chromosome-number-ancestor chromosomes, as often happens in distant hybrids [3,4,65].…”

Section: Discussionmentioning

confidence: 98%

“…Interspecific hybridization, often accompanied by the formation of allopolyploid genomes, allows plants to bypass the prohibitions on sympatric speciation known from the synthetic theory of evolution [1,2]. Various genetic processes, such as the loss of all or part of the chromosomes of one of the parents, the expansion of transposons, translocations and transpositions, and the loss of a part of the genes of one or both parental genomes, take place in complicated allopolyploid genomes [3,4]. Interspecific hybridization creates unique opportunities for effective positive selection and can be accompanied by saltation speciation [5,6].…”

Section: Introductionmentioning

confidence: 99%

Intragenomic Polymorphism of the ITS 1 Region of 35S rRNA Gene in the Group of Grasses with Two-Chromosome Species: Different Genome Composition in Closely Related Zingeria Species

Rodionov

Gnutikov

Носов

et al. 2020

Plants

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Zingeria (Poaceae) is a small genus that includes Z. biebersteiniana, a diploid species with the lowest chromosome number known in plants (2n = 4) as well as hexaploid Z. kochii and tetraploid Z. pisidica, and/or Z. trichopoda species. The relationship between these species and the other low-chromosomes species Colpodium versicolor are unclear. To explore the intragenomic polymorphism and genome composition of these species we examined the sequences of the internal transcribed spacer 1 of the 35S rRNA gene via NGS approach. Our study revealed six groups of ribotypes in Zingeria species. Their distribution confirmed the allopolyploid nature of Z. kochii, whose probable ancestors were Colpodium versicolor and Z. pisidica. Z. pisidica has 98% of rDNA characteristic only for this species, and about 0.3% of rDNA related to that of Z. biebersteiniana. We assume that hexaploid Z. kochii is either an old allopolyploid or a homodiploid that has lost most of the rRNA genes obtained from Z. biebersteiniana. In Z. trichopoda about 81% of rDNA is related to rDNA of Z. biebersteiniana and 19% of rDNA is derived from Poa diaphora sensu lato. The composition of the ribotypes of the two plants determined by a taxonomy specialist as Z. pisidica and Z. trichopoda is very different. Two singleton species are proposed on this base with ribotypes as discriminative characters. So, in all four studied Zingeria species, even if the morphological difference among the studied species was modest, the genomic constitution was significantly different, which suggests that these are allopolyploids that obtained genomes from different ancestors.

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“…It is well known that genome doubling as a result of duplications (autopoploidy) or hybridization and subsequent polyploidization (alloploidy) leads to marked changes in plant phenotype (Finigan et al, 2012;Romanov, Pimonov, 2018;Rodionov et al, 2019). These changes in plants occur both at the cellular level (Liu et al, 2018) and at the organ level (Robinson et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

“…In this regard, it is extremely important that the natural differentiation of one or another genus, the relationship between species, are reflected with high accuracy by a detailed taxonomy (Dorofeev, 1985). It should be noted, that for most of the plants important for agriculture, the volumes of the genus and species have not been unambiguously described yet (Rodionov et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Automatic morphology phenotyping of tetra- and hexaploid wheat spike using computer vision methods

et al. 2021

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Intraspecific classification of cultivated plants is necessary for the conservation of biological diversity, study of their origin and their phylogeny. The modern cultivated wheat species originated from three wild diploid ancestors as a result of several rounds of genome doubling and are represented by di-, tetra- and hexaploid species. The identification of wheat ploidy level is one of the main stages of their taxonomy. Such classification is possible based on visual analysis of the wheat spike traits. The aim of this study is to investigate the morphological characteristics of spikes for hexa- and tetraploid wheat species based on the method of high-performance phenotyping. Phenotyping of the quantitative characteristics of the spike of 17 wheat species (595 plants, 3348 images), including eight tetraploids (Triticum aethiopicum, T. dicoccoides, T. dicoccum, T. durum, T. militinae, T. polonicum, T. timopheevii, and T. turgidum) and nine hexaploids (T. compactum, T. aestivum, i:ANK-23 (near-isogenic line of T. aestivum cv. Novosibirskaya 67), T. antiquorum, T. spelta (including cv. Rother Sommer Kolben), T. petropavlovskyi, T. yunnanense, T. macha, T. sphaerococcum, and T. vavilovii), was performed. Wheat spike morphology was described on the basis of nine quantitative traits including shape, size and awns area of the spike. The traits were obtained as a result of image analysis using the WERecognizer program. A cluster analysis of plants according to the characteristics of the spike shape and comparison of their distributions in tetraploid and hexaploid species showed a higher variability of traits in hexaploid species compared to tetraploid ones. At the same time, the species themselves form two clusters in the visual characteristics of the spike. One type is predominantly hexaploid species (with the exception of one tetraploid, T. dicoccoides). The other group includes tetraploid ones (with the exception of three hexaploid ones, T. compactum, T. antiquorum, T. sphaerococcum, and i:ANK-23). Thus, it has been shown that the morphological characteristics of spikes for hexaploid and tetraploid wheat species, obtained on the basis of computer analysis of images, include differences, which are further used to develop methods for plant classifications by ploidy level and their species in an automatic mode.

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Genetic Consequences of Interspecific Hybridization, Its Role in Speciation and Phenotypic Diversity of Plants

Cited by 26 publications

References 132 publications

Duckweed Species Genotyping and Interspecific Hybrid Discovery by Tubulin-Based Polymorphism Fingerprinting

Duckweed Species Genotyping and Interspecific Hybrid Discovery by Tubulin-Based Polymorphism Fingerprinting

Intragenomic Polymorphism of the ITS 1 Region of 35S rRNA Gene in the Group of Grasses with Two-Chromosome Species: Different Genome Composition in Closely Related Zingeria Species

Automatic morphology phenotyping of tetra- and hexaploid wheat spike using computer vision methods

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