Genetic Confirmation of Mungbean (Vigna radiata) and Mashbean (Vigna mungo) Interspecific Recombinants using Molecular Markers

Abbas, Ghulam; Hameed, Amjad; Rizwan, Muhammad; Ahsan, Muhammad; Asghar, Muhammad; Iqbal, Nayyer

doi:10.3389/fpls.2015.01107

Cited by 15 publications

(7 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mungbean crop in Australia consists of two main crop types, one with green seeds called green gram (Vigna radiata Wilczek) and another with black grains called black gram (Vigna mungo Hepper). Both species are cross compatible to a limited extent [16]. In this review, the primary emphasis is on green gram, which is the dominant type of mungbean grown in Australia.…”

Section: Mungbean Evolution and Diversitymentioning

confidence: 99%

Physiological and Agronomic Strategies to Increase Mungbean Yield in Climatically Variable Environments of Northern Australia

Chauhan¹,

Williams²

2018

Agronomy

View full text Add to dashboard Cite

Mungbean (Vigna radiata (L.) Wilczek) in Australia has been transformed from a niche opportunistic crop into a major summer cropping option for dryland growers in the summer-dominant rainfall regions of Queensland and New South Wales. This transformation followed stepwise genetic improvements in both grain yields and disease resistance. For example, more recent cultivars such as 'Crystal', 'Satin II', and 'Jade-AU' have provided up to a 20% yield advantage over initial introductions. Improved agronomic management to enable mechanised management and cultivation in narrow (<50 cm) rows has further promised to increase yields. Nevertheless, average yields achieved by growers for their mungbean crops remain less than 1 t/ha, and are much more variable than other broad acre crops. Further increases in yield and crop resilience in mungbean are vital. In this review, opportunities to improve mungbean productivity have been analysed at four key levels including phenology, leaf area development, dry matter accumulation, and its partitioning into grain yield. Improving the prediction of phenology in mungbean may provide further scope for genetic improvements that better match crop duration to the characteristics of target environments. There is also scope to improve grain yields by increasing dry matter production through the development of more efficient leaf canopies. This may introduce additional production risks as dry matter production depends on the amount of available water, which varies considerably within and across growing regions in Australia. Improving crop yields by exploiting G × E × M interactions related to cultivar photo-thermal sensitivities and make better use of available water in these variable environments is likely to be a less risky strategy. Improved characterisation of growing environments using modelling approaches could also better define and identify the risks of major abiotic constraints. This would assist in optimising breeding and management strategies to increase grain yield and crop resilience in mungbean for the benefit of growers and the industry.

show abstract

Section: Mungbean Evolution and Diversitymentioning

confidence: 99%

Physiological and Agronomic Strategies to Increase Mungbean Yield in Climatically Variable Environments of Northern Australia

Chauhan¹,

Williams²

2018

Agronomy

View full text Add to dashboard Cite

show abstract

“…SRAP markers amplify open reading frames and display a co-dominant inheritance mode, which makes it easier for hybrid identification. This hybrid identification method could further be augmented with additional morphological variability as has been previously demonstrated for C. oleifera (Fan et al 2011;Peng S et al 2011;Peng F 2012;Huang et al 2014;Abbas et al 2015;Yu et al 2016;. In the present study, we used 17 SRAP primes which resulted in stable amplification and produced unambiguous highly polymorphic DNA fragments (average % polymorphic bands (PPB) across all accessions = 91.60 % (Table 2)).…”

Section: Yield and Fruit Indicators Relationship With Genetic Distancmentioning

confidence: 76%

Marker-assisted selection in C. oleifera hybrid population

et al. 2020

View full text Add to dashboard Cite

Marker-assisted selection (MAS) is implemented to improve Camellia oleifera yield and fruit attributes for meeting China’s increased demand for new varieties development. We conducted three-generational (G0, G1, and G2) hybridization (diallel mating) and selection experiment and used Sequence-Related Amplified Polymorphism (SRAP). SRAP markers to investigate their utility in a MAS framework. The utilized SRAP markers were instrumental in hybrid authenticity and the identification of matroclinal or patroclinal inheritance presence, thus guiding mating pair selection and direction (their role as male or females). Across the studied 3 generations, estimates of genetic diversity parameters showed steady increase with percentage increase of ((G0 to G1 and G1 to G2) 9.25 and 9.05: observed number of alleles; 3.12 and 7.80: means effective number of alleles; 12.35 and 22.34: Nei‘s gene diversity; and 14.21 and 21.77: Shannon‘s index), indicating lack of diversity reduction associated with selection. Estimates of genetic distance and their correlation with heterosis were useful in guiding selection of mating pairs for achieving the desired yield and fruit attributes (fruit diameter, height, weight, and index, peel thickness, number of seeds per fruit, seed weight per fruit, and seed rate). Most yield and fruit attributes exhibited high broad-sense heritability with increasing trend over generation intervals, indicating the increased potential of hybrid breeding for this species.

show abstract

“…This is highly relevant for molecular breeding to produce high yield in hybrid cultivar of Vigna (47). Similarly, Vigna SSR has also been used for PCR-based confirmation of successful production of interspecific recombinants of two species namely, mungbean ( V. radiata ) and mashbean ( V. mungo ) (48). SSR has been successfully used to identify tightly linked yellow mosaic virus resistance gene (49).…”

Section: Discussionmentioning

confidence: 99%

OUP accepted manuscript

et al. 2019

View full text Add to dashboard Cite

Genus Vigna represented by more than 100 species is a source of nutritious edible seeds and sprouts that are rich sources of protein and dietary supplements. It is further valuable because of therapeutic attributes due to its antioxidant and anti-diabetic properties. A highly diverse and an extremely ecological niche of different species can be valuable genomic resources for productivity enhancement. It is one of the most underutilized crops for food security and animal feeds. In spite of huge species diversity, only three species of Vigna have been sequenced; thus, there is a need for molecular markers for the remaining species. Computational approach of microsatellite marker discovery along with evaluation of polymorphism utilizing available genomic data of different genotypes can be a quick and an economical approach for genomic resource development. Cross-species transferability by e-PCR over available genomes can further prioritize the potential SSR markers, which could be used for genetic diversity and population differentiation of the remaining species saving cost and time. We present VigSatDB —the world’s first comprehensive microsatellite database of genus Vigna , containing >875 K putative microsatellite markers with 772 354 simple and 103 865 compound markers mined from six genome assemblies of three Vigna species, namely, Vigna radiata (Mung bean), Vigna angularis (Adzuki bean) and Vigna unguiculata (Cowpea). It also contains 1976 validated published markers. Markers can be selected on the basis of chromosomes/location specificity, and primers can be generated using Primer3core tool integrated at backend. Efficacy of VigSatDB for microsatellite loci genotyping has been evaluated by 15 markers over a panel of 10 diverse genotype of V. radiata . Our web genomic resources can be used in diversity analysis, population and varietal differentiation, discovery of quantitative trait loci/genes, marker-assisted varietal improvement in endeavor of Vigna crop productivity and management.

show abstract

Genetic Confirmation of Mungbean (Vigna radiata) and Mashbean (Vigna mungo) Interspecific Recombinants using Molecular Markers

Cited by 15 publications

References 21 publications

Physiological and Agronomic Strategies to Increase Mungbean Yield in Climatically Variable Environments of Northern Australia

Physiological and Agronomic Strategies to Increase Mungbean Yield in Climatically Variable Environments of Northern Australia

Marker-assisted selection in C. oleifera hybrid population

OUP accepted manuscript

Contact Info

Product

Resources

About