Genetic Composition of Laboratory Stocks of the Self-Fertilizing Fish Kryptolebias marmoratus: A Valuable Resource for Experimental Research

Tatarenkov, Andrey; Ring, Brian C.; Elder, John F.; Bechler, David L.; Avise, John C.

doi:10.1371/journal.pone.0012863

Cited by 84 publications

(86 citation statements)

References 62 publications

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“…The study fish had two different selfing backgrounds: the R strain was derived originally from Belize in the early 1990s, and the DAN strain was also derived from Belize but in the early 2000s [26]. These strains were chosen due to known differences in their levels of genotypic diversity (i.e.…”

Section: Materials and Methods (A) Sex Ratios Of Kryptolebias Marmoratmentioning

confidence: 99%

Epigenetic regulation of sex ratios may explain natural variation in self-fertilization rates

Ellison¹,

López

Morán

et al. 2015

Proc. R. Soc. B.

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Self-fertilization (selfing) favours reproductive success when mate availability is low, but renders populations more vulnerable to environmental change by reducing genetic variability. A mixed-breeding strategy (alternating selfing and outcrossing) may allow species to balance these needs, but requires a system for regulating sexual identity. We explored the role of DNA methylation as a regulatory system for sex-ratio modulation in the mixed-mating fish Kryptolebias marmoratus. We found a significant interaction between sexual identity (male or hermaphrodite), temperature and methylation patterns when two selfing lines were exposed to different temperatures during development. We also identified several genes differentially methylated in males and hermaphrodites that represent candidates for the temperature-mediated sex regulation in K. marmoratus. We conclude that an epigenetic mechanism regulated by temperature modulates sexual identity in this selfing species, providing a potentially widespread mechanism by which environmental change may influence selfing rates. We also suggest that K. marmoratus, with naturally inbred populations, represents a good vertebrate model for epigenetic studies.

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Section: Materials and Methods (A) Sex Ratios Of Kryptolebias Marmoratmentioning

confidence: 99%

Epigenetic regulation of sex ratios may explain natural variation in self-fertilization rates

Ellison¹,

López

Morán

et al. 2015

Proc. R. Soc. B.

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“…Kryptolebias marmoratus hermaphrodites at least 6 months of age (Slc8E strain) (Tatarenkov et al, 2010) were obtained from the breeding colony maintained in the Hagen Aqualab, University of Guelph. Fish were reared individually in 120 ml semi-transparent plastic containers (FisherBrand…”

Section: Experimental Animalsmentioning

confidence: 99%

The amphibious fishKryptolebias marmoratususes alternate strategies to maintain oxygen delivery during aquatic hypoxia and air exposure

Turko

Robertson

Bianchini

et al. 2014

Journal of Experimental Biology

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Despite the abundance of oxygen in atmospheric air relative to water, the initial loss of respiratory surface area and accumulation of carbon dioxide in the blood of amphibious fishes during emersion may result in hypoxemia. Given that the ability to respond to low oxygen conditions predates the vertebrate invasion of land, we hypothesized that amphibious fishes maintain O 2 uptake and transport while emersed by mounting a co-opted hypoxia response. We acclimated the amphibious fish Kryptolebias marmoratus, which are able to remain active for weeks in both air and water, for 7 days to normoxic brackish water (15‰, ~21 kPa O 2 ; control), aquatic hypoxia (~3.6 kPa), normoxic air (~21 kPa) or aerial hypoxia (~13.6 kPa). Angiogenesis in the skin and bucco-opercular chamber was pronounced in air-versus water-acclimated fish, but not in response to hypoxia. Aquatic hypoxia increased the O 2 -carrying capacity of blood via a large (40%) increase in red blood cell density and a small increase in the affinity of hemoglobin for O 2 (P 50 decreased 11%). In contrast, air exposure increased the hemoglobin O 2 affinity (decreased P 50 ) by 25% without affecting the number of red blood cells. Acclimation to aerial hypoxia both increased the O 2 -carrying capacity and decreased the hemoglobin O 2 affinity . These results suggest that O 2 transport is regulated both by O 2 availability and also, independently, by air exposure. The ability of the hematological system to respond to air exposure independent of O 2 availability may allow extant amphibious fishes, and may also have allowed primitive tetrapods to cope with the complex challenges of aerial respiration during the invasion of land.KEY WORDS: Hemoglobin, Oxygen-carrying capacity, Hemoglobin-oxygen affinity, Air-breathing organ, Air-breathing fish, Mangrove rivulus INTRODUCTIONThe transition from aquatic to terrestrial life represents a major step in vertebrate evolution because the physical conditions between these environments are dramatically different. Oxygen solubility is relatively low in water and even well-oxygenated aquatic environments contain only about 3% of the O 2 available in atmospheric air (Dejours, 1988). Low concentrations of aquatic O 2 , particularly in hypoxic habitats, have often been hypothesized to be one of the driving forces behind the evolution of amphibious or terrestrial life histories because invasion of land would allow animals to exploit the O 2 -rich aerial environment (Graham, 1997). RESEARCH ARTICLEDepartment of Integrative Biology, University of Guelph, Guelph, ON N1G 2W1, Canada.*Author for correspondence (aturko@uoguelph.ca) Received 4 July 2014; Accepted 15 September 2014Taking advantage of aerial O 2 presents several challenges for fishes. Water-breathing fishes exchange respiratory gases across the gills but during emersion the gill lamellae typically collapse and coalesce, reducing the surface area available for respiration. Accumulation of CO 2 in the blood of emersed fishes, resulting from the low solubility of CO 2 in...

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“…This clonal line is homozygous at all loci tested by microsatellite analysis and is identical to all laboratories that culture it (Tatarenkov et al, 2010). Hermaphroditic individuals were kept individually in 1.5-L plastic aquaria in a controlled environment (25 6 1 C; 14% salinity; 12 hr:12 hr light:dark photoperiod), and were fed daily ad libitum on Artemia.…”

Section: Experimental Procedures Experimental Animalsmentioning

confidence: 99%

“…This reproductive mode provides a genetic architecture composed mainly of homozygous inbred strains, and a low percentage of highly heterozygous individuals induced by outcrossing, providing the basis for the formation of new clonal lines (Mackiewicz et al, 2006). Recently, 21 distinct clonal lines have been genetically verified (Tatarenkov et al, 2010). The Hon9 line, used in the present study, is amongst these distinct and established clonal lines.…”

Section: Introductionmentioning

confidence: 99%

Embryonic development of the self‐fertilizing mangrove killifish Kryptolebias marmoratus

Mourabit

Edenbrow

Croft

et al. 2011

Developmental Dynamics

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The mangrove killifish, Kryptolebias marmoratus, is a self-fertilizing vertebrate offering vast potential as a model species in many biological disciplines. Previous studies have defined developmental stages but lacked visual representations of the various embryonic structures. We offer detailed photographic images of K. marmoratus development with revised descriptions. An improved dechorionation method was developed to provide high resolution photographs, in addition to a microinjection technique enabling cell marking in the yolk syncytial layer. Embryos were also treated with PTU (1-phenyl 2-thiourea), an inhibitor of melanogenesis, to provide optical transparency revealing internal structures in late stages of development. Chemical exposures (PTU and retinoic acid) demonstrated that K. marmoratus embryos were sensitive to chemicals, illustrating further their usefulness in developmental biology studies. Our data suggest that K. marmoratus embryos are easily used and manipulated, supporting the use of this hermaphroditic vertebrate as a strong comparative model system in embryology, evolution, genetics, environmental and medical biology. Developmental Dynamics 240:1694-1704,

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Genetic Composition of Laboratory Stocks of the Self-Fertilizing Fish Kryptolebias marmoratus: A Valuable Resource for Experimental Research

Cited by 84 publications

References 62 publications

Epigenetic regulation of sex ratios may explain natural variation in self-fertilization rates

Epigenetic regulation of sex ratios may explain natural variation in self-fertilization rates

The amphibious fishKryptolebias marmoratususes alternate strategies to maintain oxygen delivery during aquatic hypoxia and air exposure

Embryonic development of the self‐fertilizing mangrove killifish Kryptolebias marmoratus

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