Genetic comparison between Spirometra erinacei and S. mansonoides using PCR-RFLP analysis

Abstract

The only observed morphological difference between Spirometra erinacei and S. mansonoides is the uterine shape of the mature proglottid. Two species of worms are thought to be evolutionarily closely related. Biomolecular comparison of the two worms by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was conducted to observe the genetic distance. The 28s rDNA, mitochondrial cytochrome c oxidase subunit I (mCOI), and ribosomal internal transcribed spacer 1 (ITS1) fragments were obtained from the worms by PCR. The PCR products were cleaved by 5 four-base pair restriction enzyme combinations (Msp I, Hae III, Alu I, Cfo I, Rsa I), electrophoresed and analyzed with PAUP 3.1.1. The fragment patterns of 28S rDNA and ITS1 demonstrated that two worms were in identical systematic tree with bootstrap number 94 and 100, respectively. As for mCOI, bootstrap number was 74 in a different tree. Above results are indicative of recent common ancestry between S. erinacei and S. mansonoides.

Molecular Detection of Spirometra decipiens in the United States

“…PRBA333), which was deposited by one of the authors, Dr. Woon-Mok Sohn of the Department of Parasitology and Tropical Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju, Korea. The specimen history began from eggs of S. mansonoides originating from Dr. C. K. Phares of the Department of Biochemistry and Molecular Biology, University of Nebraska, USA [ 8 ]. The eggs were incubated in the laboratory to establish an experimental life cycle using cyclops, tadpoles and frogs, and a cat in Korea (Sohn WM.…”

“…Iwata [ 7 ], in 1972, argued that the morphological difference in uterine shape relative to the mature proglottids of S. erinaceieuropaei is due to differences in developmental stages but not specific characters. Later, a molecular difference was observed between S. erinaceieuropaei and S. mansonoides using PCR-RFLP and cox1 sequence analysis [ 8 ]. More recently, human sparganosis was identified by morphological and genetic analyses in Korea, and the complete mitochondrial genomes of S. erinaceieuropaei and S. decipiens isolated from Korea have been recorded and compared [ 9 , 10 ].…”

Application of polymerase chain reaction (PCR) in parasitology

“…Η μόνη μορφολογική διαφορά που εντοπίζεται είναι το σχή μα της μήτρας στην ώριμη προγλωττίδα 42 . Η γενετική από σταση μεταξύ των δυο αυτών ειδών προσδιορίζεται με ε φαρμογή της RFLP-PCR 42 .…”

“…Η μόνη μορφολογική διαφορά που εντοπίζεται είναι το σχή μα της μήτρας στην ώριμη προγλωττίδα 42 . Η γενετική από σταση μεταξύ των δυο αυτών ειδών προσδιορίζεται με ε φαρμογή της RFLP-PCR 42 . Τα αποτελέσματα δείχνουν ό τι οι δυο σκώληκες μοιράζονται ένα κοινό πρόγονο στο ά μεσο παρελθόν τους 42 .…”

“…Η γενετική από σταση μεταξύ των δυο αυτών ειδών προσδιορίζεται με ε φαρμογή της RFLP-PCR 42 . Τα αποτελέσματα δείχνουν ό τι οι δυο σκώληκες μοιράζονται ένα κοινό πρόγονο στο ά μεσο παρελθόν τους 42 .…”