Abstract:Pseudomonas putida KT2440
is an
emerging microbial chassis for biobased chemical production from renewable
feedstocks and environmental bioremediation. However, tools for studying,
engineering, and modulating protein complexes and biosynthetic enzymes
in this organism are largely underdeveloped. Genetic code expansion
for the incorporation of unnatural amino acids (unAAs) into proteins
can advance such efforts and, furthermore, enable additional controls
of biological processes of the strain. In this work, we … Show more
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