Genetic (co)variation in resistance of Pacific white shrimp Litopenaeus vannamei to acute hepatopancreatic necrosis disease (AHPND) and white spot syndrome virus (WSSV) in challenge tests

Campos-Montes, Gabriel R.; Caballero-Zamora, Alejandra; Montaldo, Hugo H.; Montoya‐Rodríguez, Leobardo; Sala, B.; Soto-Rodríguez, Sonia A.; Ortega, Alfonso Martínez; Quintana-Casares, Juan C.; Castillo‐Juárez, Héctor

doi:10.1016/j.aquaculture.2020.734994

Cited by 9 publications

(4 citation statements)

References 26 publications

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“…Compared to growth traits, fewer studies have reported on the assessment of genetic parameters for WSSV resistance in L. vannamei . For example, Gitterle et al [ 15 ], Caballero-Zamora et al [ 16 ], and Campos-Montes et al [ 12 ] adopted the traditional BLUP method to assess the heritability of WSSV survival traits in L. vannamei and found low heritability estimates of 0.03–0.20 for WSSV survival traits. In this study, the heritability of the WSSV resistance trait in L. vannamei assessed based on the pBLUP method was not significantly different from 0 ( p > 0.05).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, when both pedigree and genotype information were combined for assessment based on the ssGBLUP method, prediction accuracy increased by 122.58%, bias decreased by 50.12%, and the resulting heritability was 0.198 ± 0.066. Campos-Montes et al [ 12 ] documented the heritabilities concerning the time of death post-WSSV infection and binary survival using GBLUP and ssGBLUP, which ranged from 0.094 to 0.095 and 0.085 to 0.105, respectively, employing a commercial 50 K SNP panel in post-larvae. Discrepancies in heritability estimates may stem from various factors, including the experimental population, population age, and infection methods.…”

Section: Discussionmentioning

confidence: 99%

“…The precise estimation of genetic parameters for objective traits is fundamental to breeding programs, particularly in guiding the formulation of selection indices, breeding stock retention, and mating strategies. Numerous studies have documented the heritability levels of shrimp resistance to WSSV, ranging from 0.03 to 0.53 [ 3 , 11 , 12 , 13 , 14 ]. The genetic correlations between growth and WSSV resistance in different populations ranged from negative to no significant association [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Estimation of Genetic Parameters for Growth and WSSV Resistance Traits in Litopenaeus vannamei

Sui,

Sun,

Kong

et al. 2024

Animals

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The current study aimed to provide a precise assessment of the genetic parameters associated with growth and white spot syndrome virus (WSSV) resistance traits in Pacific white shrimp (Litopenaeus vannamei). This was achieved through a controlled WSSV challenge assay and the analysis of phenotypic values of five traits: body weight (BW), overall length (OL), body length (BL), tail length (TL), and survival hour post-infection (HPI). The analysis included test data from a total of 1017 individuals belonging to 20 families, of which 293 individuals underwent whole-genome resequencing, resulting in 18,137,179 high-quality SNP loci being obtained. Three methods, including pedigree-based best linear unbiased prediction (pBLUP), genomic best linear unbiased prediction (GBLUP), and single-step genomic BLUP (ssGBLUP) were utilized. Compared to the pBLUP model, the heritability of growth-related traits obtained from GBLUP and ssGBLUP was lower, whereas the heritability of WSSV resistance was higher. Both the GBLUP and ssGBLUP models significantly enhanced prediction accuracy. Specifically, the GBLUP model improved the prediction accuracy of BW, OL, BL, TL, and HPI by 4.77%, 21.93%, 19.73%, 19.34%, and 63.44%, respectively. Similarly, the ssGBLUP model improved prediction accuracy by 10.07%, 25.44%, 25.72%, 19.34%, and 122.58%, respectively. The WSSV resistance trait demonstrated the most substantial enhancement using both genomic prediction models, followed by body size traits (e.g., OL, BL, and TL), with BW showing the least improvement. Furthermore, the choice of models minimally impacted the assessment of genetic and phenotypic correlations. Genetic correlations among growth traits ranged from 0.767 to 0.999 across models, indicating high levels of positive correlations. Genetic correlations between growth and WSSV resistance traits ranged from (−0.198) to (−0.019), indicating low levels of negative correlations. This study assured significant advantages of the GBLUP and ssGBLUP models over the pBLUP model in the genetic parameter estimation of growth and WSSV resistance in L. vannamei, providing a foundation for further breeding programs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Estimation of Genetic Parameters for Growth and WSSV Resistance Traits in Litopenaeus vannamei

Sui,

Sun,

Kong

et al. 2024

Animals

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“…Precise dose calculation and normalisation for per os inoculation can be further challenged by inconsistent viral particle distribution within the tissue and variable feeding behaviour of the experimental shrimp (also see Section 3.3.3 ‘Inoculation method’). Maceration or homogenisation of tissue samples for per os challenge can minimise the influence of heterogeneous viral particle distribution on dosage 182–184 . Additionally, implementing a starving period for experimental animals before per os challenge can increase shrimp receptivity to the inoculum feed, improving dose consistency 57,159,185 …”

Section: Essential Components Of Viral Pathogen Challenge Experiments...mentioning

confidence: 99%

Towards a ‘systems’ approach for viral challenge experiments in shrimp: Reporting guidelines for publication

Arbon,

Andrade Martinez,

Jerry

et al. 2023

Reviews in Aquaculture

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The success of contemporary disease management strategies in shrimp aquaculture, such as the ‘systems’ approach, is predicated on robust knowledge of the conditions and interactions between the host, pathogen and environment that promote disease. Pathogen challenge experiments (PCEs) are a power tool for investigating these conditions and interactions. However, absence of accurately reported experimental detail in published PCEs limits scientific transparency, reproducibility, and the potential for the research to make progressive advancements contributing to contemporary shrimp disease management strategies. This review identifies and discusses key factors relating to the host (shrimp), pathogen (virus), and environment that should be carefully considered during the design and publication of PCEs. We offer substantial evidence of their impact on viral disease outcomes, drawn from the existing body of literature, to supporting their consideration. The prevalence of reported experimental details for these factors across 186 viral PCEs in shrimp were evaluated. The review highlights a concerning paucity of experimental detail reported in published shrimp PCEs. We propose a checklist for the minimum reportable information in the publication of shrimp viral PCEs, hereafter referred to as the Shrimp PCE Reporting Guidelines (SPERG). The guidelines aim to enhance the transparency and standardisation of reporting in published PCEs, ensuring that key factors pertaining to the shrimp, pathogen, and environment are adequately considered and documented. Adoption of SPERG is envisaged to empower researchers, reviewers, and readers to assess the internal and external validity of PCEs, facilitating critical evaluation and improved utility of PCE findings for contemporary disease management.

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Genetic (co)variation for resistance to white spot syndrome virus, acute hepatopancreatic necrosis, and overall pond survival in Litopenaeus vannamei

López-Ordaz,

Caballero-Zamora,

Yáñez

et al. 2024

Aquacult Int

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Genetic (co)variation in resistance of Pacific white shrimp Litopenaeus vannamei to acute hepatopancreatic necrosis disease (AHPND) and white spot syndrome virus (WSSV) in challenge tests

Cited by 9 publications

References 26 publications

Estimation of Genetic Parameters for Growth and WSSV Resistance Traits in Litopenaeus vannamei

Estimation of Genetic Parameters for Growth and WSSV Resistance Traits in Litopenaeus vannamei

Towards a ‘systems’ approach for viral challenge experiments in shrimp: Reporting guidelines for publication

Genetic (co)variation for resistance to white spot syndrome virus, acute hepatopancreatic necrosis, and overall pond survival in Litopenaeus vannamei

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