Genetic characterization of Western European noble crayfish populations (Astacus astacus) for advanced conservation management strategies

Schrimpf, Anne; Piscione, M.; Cammaerts, Roger; Collas, Marc; Herman, D. M.; Jung, A Young; Ottburg, F.G.W.A.; Roessink, Ivo; Xavier, Raquel; Schulz, Ralf; Theissinger, Kathrin

doi:10.1007/s10592-017-0981-3

Cited by 17 publications

(26 citation statements)

References 48 publications

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“…SSR are highly polymorphic, abundant and evenly distributed throughout genomes (Schlötterer, 2004). This feature makes them a popular marker to analyse genetic diversity (Groeneveld et al, 2010), including of noble crayfish populations (Gross et al, 2013; Kõiv et al., 2008; Kõiv et al., 2009; Schrimpf et al, 2014; Schrimpf et al, 2017). Microsatellites provide many advantages, but they also have shortcomings.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity in natural populations of noble crayfish (Astacus astacusL.) in north-western Poland on the basis of combined SSR and AFLP data

Panicz

Napora-Rutkowski

Keszka

et al. 2019

PeerJ

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Background Conservation of noble crayfish (Astacus astacus) populations is becoming particularly important since the number of individuals is rapidly declining across the distribution range of the species in Europe. Five crayfish populations in northwestern Poland have been constantly monitored for two decades. However, the genetic structure of these populations has not been analysed, although this information is important to devise effective conservation strategies. Methods Noble crayfish were collected in the autumn of 2014 by scuba diving in Lakes Graniczne, Babinki, Biwakowe, Sęki and Kwisno, all of which are situated in the Bytów Lakeland of northwestern Poland. Genetic diversity of the five populations was assessed based on allele variability in nine SSR regions and six AFLP primer combinations. Results Microsatellite results analysed with AMOVA showed that the diversity between populations corresponds to 18% of total variability, which was confirmed by similar results obtained using AFLP. Additionally, significant genetic diversity was revealed by high average FST values. All of the studied crayfish populations significantly deviated from the expected Hardy–Weinberg genetic equilibrium and were characterised by negative values of inbreeding coefficient (FIS). Discussion The invariably negative inbreeding coefficients (FIS) suggest a low number of mating individuals, a possible consequence of the phenomenon known as genetic bottleneck. However, additional comprehensive analyses are needed to assess the genetic structure, origin and vulnerability of the remaining populations of noble crayfish in the Bytów Lakeland of northwestern Poland, which have high conservation value and are particularly important as a live genetic bank for breeding and restitution purposes.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity in natural populations of noble crayfish (Astacus astacusL.) in north-western Poland on the basis of combined SSR and AFLP data

Panicz

Napora-Rutkowski

Keszka

et al. 2019

PeerJ

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“…Geographically and genetically isolated phylogroups represent the evolutionary legacy of A. torrentium which is highly relevant for conservation due to their mostly small distribution ranges and evolutionary distinctness. Since human mediated translocation and restocking of crayfish for repopulation are encouraged with the aim of increasing the genetic diversity of endangered populations [ 115 ], future conservation programs should consider conducting translocations and repopulations only within the same ESU [ 46 , 116 – 118 ].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, one of the fundamental issues in the conservation of freshwater species is in maintaining genetic diversity by defining the degree of connectivity between populations [ 119 ] and finding a balance between outbreeding and inbreeding depression that represent potential threat while restocking/repopulating, so future research should be focused on the study of the genetic structure of phylogroups. Population genetic analyses based on microsatellites can contribute to the understanding of the degree of genetic variation within and among populations, potentially identify management units (MUs) and source populations for future introductions, as well as to reveal recent evolutionary changes and possible population-level hybridisation events through secondary contacts [ 46 , 50 , 117 , 118 , 120 ]. In addition, cytogenetic research, next generation sequencing and genomic approaches may advance understanding of phylogenetic relationships and taxonomic status of mt and nuDNA phylogroups which, without doubt, play a pivotal role in long term future evolution of A. torrentium .…”

Section: Discussionmentioning

confidence: 99%

New insights into the genetic diversity of the stone crayfish: taxonomic and conservation implications

et al. 2020

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Background Austropotamobius torrentium is a freshwater crayfish species native to central and south-eastern Europe, with an intricate evolutionary history and the highest genetic diversity recorded in the northern-central Dinarides (NCD). Its populations are facing declines, both in number and size across its entire range. By extanding current knowledge on the genetic diversity of this species, we aim to assist conservation programmes. Multigene phylogenetic analyses were performed using different divergence time estimates based on mitochondrial and, for the first time, nuclear DNA markers on the largest data set analysed so far. In order to reassess taxonomic relationships within this species we applied several species delimitation methods and studied the meristic characters with the intention of finding features that would clearly separate stone crayfish belonging to different phylogroups. Results Our results confirmed the existence of high genetic diversity within A. torrentium, maintained in divergent phylogroups which have their own evolutionary dynamics. A new phylogroup in the Kordun region belonging to NCD has also been discovered. Due to the incongruence between implemented species delimitation approaches and the lack of any morphological characters conserved within lineages, we are of the opinion that phylogroups recovered on mitochondrial and nuclear DNA are cryptic subspecies and distinct evolutionary significant units. Conclusions Geographically and genetically isolated phylogroups represent the evolutionary legacy of A. torrentium and are highly relevant for conservation due to their evolutionary distinctiveness and restricted distribution.

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“…This sympatric occurrence could be the result of secondary contact of two different recolonisation lineages, as for Europe different potential ice age refugia for freshwater species are presumed (e.g. Culling et al, ; Gum, Gross, & Kuehn, ; Nesbø, Fossheim, Vollestad, & Jakobsen, ; Schrimpf et al, ). Whereas Western and Central Europe might have been colonised by burbot from refugia in France or Italy, Northern and Eastern Europe are resumed to have been colonised from refugia from the Pontic regions and Central Asia (Costedoat & Gilles, ).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and population structure of burbot Lota lota in Germany: Implications for conservation and management

Wetjen

Schmidt

Schrimpf

et al. 2019

Fisheries Management Eco

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The genetic structure of the gadiform fish species, burbot Lota lota L., was investigated across Germany to derive management options for facilitating the preservation of genetic diversity. Sequence analysis of the mitochondrial control region (n = 244) and microsatellite analysis (n = 861) of specimens from 20 sites revealed genetic structuring between major river basins, and particularly between lake and river habitats. The admixture zone between the Eurasian and West European phylogenetic clades in Lake Constance was confirmed and expanded to include the drainage basins of the rivers Rhine and Schlei/Trave. Haplotype distribution and private haplotypes in single river basins indicated population differentiation and imply that German burbot constituted an important part of the entire species' diversity. The derived genetic structuring has implications for future stocking programmes and the preservation of the adaptive potential of burbot, a guiding species for oligotrophic lakes in Europe.

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Genetic characterization of Western European noble crayfish populations (Astacus astacus) for advanced conservation management strategies

Cited by 17 publications

References 48 publications

Genetic diversity in natural populations of noble crayfish (Astacus astacusL.) in north-western Poland on the basis of combined SSR and AFLP data

Genetic diversity in natural populations of noble crayfish (Astacus astacusL.) in north-western Poland on the basis of combined SSR and AFLP data

New insights into the genetic diversity of the stone crayfish: taxonomic and conservation implications

Genetic diversity and population structure of burbot Lota lota in Germany: Implications for conservation and management

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