Genetic characterization of weedy rice (Oryza sativa L.) based on morpho-physiology, isozymes and RAPD markers

Suh, Hak-Soo; Sato, Yoichiro; Morishima, Hajime

doi:10.1007/s001220050417

Cited by 138 publications

(119 citation statements)

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“…Using random amplified polymorphic DNA (RAPD) markers, Ge et al (1999) confirmed the taxonomic classification of O. glumaepatula as a distinct species and not as an American form of O. rufipogon. Isozymic markers have been widely used in the cultivated and wild Oryza species, thus elucidating the processes of the domestication of the cultivated rice, assessing the genetic diversity and genetic structure of populations associated with aspects of their life history (Second, 1982;Barbier, 1989;Grover and Pental, 1992;Suh et al, 1997;Akimoto et al, 1998;Buso et al, 1998;Gao et al, 2000a;Gao et al, 2000b;Gao et al, 2000c;Gao et al, 2002a;Gao et al, 2002b;Quesada et al, 2002;Ishikawa et al, 2005). Buso et al (1998) observed in four populations of O. glumaepatula collected at the basins of Rio Amazonas and Rio Paraguay, based on isozymes and RAPD, a pattern of greater variation among rather than within populations, suggesting a self-fertilization breeding system.…”

Section: Introductionmentioning

confidence: 99%

Assessing the genetic structure of Oryza glumaepatula populations with isozyme markers

Veasey

Cardin

Silva

et al. 2008

Braz. arch. biol. technol.

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Section: Introductionmentioning

confidence: 99%

Assessing the genetic structure of Oryza glumaepatula populations with isozyme markers

Veasey

Cardin

Silva

et al. 2008

Braz. arch. biol. technol.

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“…Microsatellites or simple sequence repeats (SSRs) are widely used markers for estimating genetic diversity in many species, including wild, weedy, and cultivated rice (Powell et al 1996;Suh et al 1997;Roy et al 2004), but to date, almost nothing has been reported about the genetic diversity, origin, and genetic background of autotetraploid rice.…”

Section: Introductionmentioning

confidence: 99%

Microsatellite Analysis of Genetic Variation and Population Genetic Differentiation in Autotetraploid and Diploid Rice

et al. 2008

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Genetic diversity and population genetic structure of autotetraploid and diploid populations of rice collected from Chengdu Institute of Biology, Chinese Academy of Sciences, were studied based on 36 microsatellite loci. Among 50 varieties, a moderate to high level of genetic diversity was observed at the population level, with the number of alleles per locus (A e ) ranging from 2 to 6 (mean 3.028) and polymorphism information content ranging from 0.04 to 0.76 (mean 0.366). The expected heterozygosity (H e ) varied from 0.04 to 0.76 (mean 0.370) and Shannon's index (I) from 0.098 to 1.613 (mean 0.649). The autotetraploid populations showed slightly higher levels of A e , H e , and I than the diploid populations. Rare alleles were observed at most of the simple sequence repeat loci in one or more of the 50 accessions, and a core fingerprint database of the autotetraploid and diploid rice was constructed. The F-statistics showed genetic variability mainly among autotetraploid populations rather than diploid populations (F st = 0.066). Cluster analysis of the 50 accessions showed four major groups. Group I contained all of the autotetraploid and diploid indica maintainer lines and an autotetraploid and its original diploid indica male sterile lines. Group II contained only the original IR accessions. Group III was more diverse than either Group II or Group IV, comprising both autotetraploid and diploid indica restoring lines. Group IV included a japonica cluster of the autotetraploid and diploid rices. Furthermore, genetic differences at the single-locus and two-locus levels, as well as components due to allelic and gametic differentiation, were revealed between autotetraploid and diploid varieties. This analysis indicated that the gene pools of diploid and autotetraploid rice were somewhat dissimilar, as variation exists that distinguishes autotetraploid 123Biochem Genet (2008) 46:248-266 DOI 10.1007 from diploid rices. Using this variation, we can breed new autotetraploid varieties with some important agricultural characters that were not found in the original diploid rice varieties.

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“…and L.P.L, personal observations). Some weedy rices probably originate from rice cultivars that reverted to weedy forms, while others are derived from hybridization between cultivars and wild rice (e.g., Cao et al, 2006;Suh et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Shared flowering phenology, insect pests, and pathogens among wild, weedy, and cultivated rice in the Mekong Delta, Vietnam: implications for transgenic rice

Cohen

Arpaia

Lan

et al. 2008

Environ. Biosafety Res.

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Many varieties of transgenic rice are under development in countries where wild and weedy relatives co-occur with the crop. To evaluate possible risks associated with pollen-mediated transgene dispersal, we conducted a two-year survey in Vietnam to examine overlapping flowering periods of rice (Oryza sativa L.), weedy rice (O. sativa), and wild rice (O. rufipogon Griff.), all of which are inter-fertile. We surveyed populations in two regions of the Mekong Delta, northern and southern, and at three sites in each of three habitats per region: fresh water, saline water, and acid sulfate soil. Weedy rice frequently flowered simultaneously with neighboring cultivated rice plants. Flowering was more seasonal in wild rice and often peaked in November and December. Peak flowering times of wild rice overlapped with adjacent rice fields at all of the saline sites and half of the acid sulfate sites. The longer flowering season of wild rice ensured that crop-to-wild gene flow was possible in fresh water habitats as well. Our second objective was to determine whether wild and weedy rice populations are exposed to pests that could be targeted by future transgenes, which may then provide fitness benefits. These populations shared many pathogen and insect herbivore species with cultivated rice (leaffolder, locust, cricket, planthoppers, rice bug, stem borer, sheath blight, blast, bacterial leaf blight, and brown spot). Damage by leaffolders and locusts was the most frequently observed insect feeding damage on all three rice types. Indicator species analysis revealed that most of the insect herbivores were associated with particular habitats, demonstrating the importance of broad geographic sampling for transgenic rice risk assessment. These survey data and the strong likelihood of gene flow from cultivated rice suggest that further studies are needed to examine the effects of transgenic traits such as resistance to pests on the abundance of wild and weedy rice.

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Genetic characterization of weedy rice (Oryza sativa L.) based on morpho-physiology, isozymes and RAPD markers

Cited by 138 publications

References 6 publications

Assessing the genetic structure of Oryza glumaepatula populations with isozyme markers

Assessing the genetic structure of Oryza glumaepatula populations with isozyme markers

Microsatellite Analysis of Genetic Variation and Population Genetic Differentiation in Autotetraploid and Diploid Rice

Shared flowering phenology, insect pests, and pathogens among wild, weedy, and cultivated rice in the Mekong Delta, Vietnam: implications for transgenic rice

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