Genetic Characterization of Three Egyptian Sweet Potato Genotypes Based on Morpho-Agronomic and Molecular Markers

Galal, Ola A.; Gendy, A. S. A.

doi:10.21608/ejgc.2018.9198

Cited by 2 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PCR reaction mixture consisted of 0.75 μl of genomic DNA (40 ng), 0.75 μl of 20 μM primer, 5 μL of 2X PCR Master mix Solution (i-TaqTM, iNtRON's Biotechnology) and 3.5 μL of sterile distilled water in a final volume of 10 μl. Amplification condition was performed according to Galal and El Gendy (2017). Amplification products were separated by electrophoresis and bands were detected on Benchtop UVtransilluminator and photographed using Doc-It TM Imaging System.…”

Section: Molecular Analysismentioning

confidence: 99%

“…Detecting genetic variation of transgenic plants is one of the purposes and criteria for their safety assessment. Random Amplified Polymorphic DNA (RAPD) marker has been used successfully as a molecular marker to characterize, identify and determine variations in nuclear genome between sweet potato genotypes (Gichuki et al, 2003;He et al, 2006;Lin et al, 2009;Moulin et al, 2012;da Silva et al, 2014;Galal and El Gendy, 2017). Also, this technique proved to be able to detect variation among individuals and to estimate the genetic diversity of somaclonal variations in various plant species (Hernandez et al, 2007;Sheidai et al, 2008;Khan et al, 2011;Nasim et al, 2012) including sweet potato (Aboulila, 2016).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhancement of somaclonal variations and genetic diversity using graphite nanoparticles (GtNPs) in sweet potato plants

Aboulila¹,

Galal²,

El-Samahy³

2018

Afr. J. Biotechnol.

View full text Add to dashboard Cite

To assay the efficiency of graphite nanoparticles (GtNPs) in sterilizing tissues and their role in enhancing genetic diversity, sweet potato is considered an important crop; hence its explants were used. In this experiment, GtNPs of 200, 400 and 800 ppm concentrations were used for sterilization of MS callus induction and regeneration media in Abees cultivar. The results showed that GtNPs had a good potential for removing bacterial contaminants without having side effects on the explant viability during the sterilization of sweet potato tissue in all their concentrations. Also, the percentage of callus induction increased from 98.67% in control to 100% in all GtNPs concentrations. The number of shoots per callus was enhanced at 400 ppm concentration. RAPD molecular markers and SDS-PAGE analysis were used to assess the genetic diversity of the sweet potato selected plants obtained from somaclonal variations in combination with GtNPs. Five decamer random amplified polymorphic DNA (RAPD) primers generated a total of 96 DNA fragments from the selected variants and their parent. Out of them, 82 polymorphic bands appeared with 85.42% polymorphism. The levels of DNA and protein patterns polymorphism within each treatment varied. RAPD and protein markers revealed that the concentration of 800 ppm showed the lowest similarity average among the ten selected variants and their parent. The obtained results indicated that somaclonal variation with GtNPs can be combined to increase the induced mutations frequency.

show abstract

Section: Molecular Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancement of somaclonal variations and genetic diversity using graphite nanoparticles (GtNPs) in sweet potato plants

Aboulila¹,

Galal²,

El-Samahy³

2018

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Morphological descriptors (CIP, 2010;Hualla et al, 2015;Huam an, 1991Huam an, , 1992 are often used to characterize phenotypes and for making selections in breeding programs (Gr€ uneberg et al, 2015;Wadl et al, 2018). A total of 104 potential descriptors were defined for sweetpotato (Huam an, 1991); however, morphoagronomic characters other than the International Plant Genetic Resources Institute sweetpotato descriptors (Huam an, 1991) also have been used by several researchers (Efisue, 2015;Galal and El Gendy, 2017;Reddy et al, 2018). A modified version of the sweetpotato descriptors is used in the GRIN database (CIP, 2010;USDA, 2019a), and has been adapted for the sweetpotato ontology (Anonymous, 2019a(Anonymous, , 2019bHualla et al, 2015).…”

mentioning

confidence: 99%

Phenotypic Variation in Leaf Morphology of the USDA, ARS Sweetpotato (Ipomoea batatas) Germplasm Collection

Jackson¹,

Harrison²,

Jarret³

et al. 2020

horts

View full text Add to dashboard Cite

During 2012–14, 737 sweetpotato, Ipomoea batatas (L.) Lam. (Convolvulaceae), plant introduction (PI) accessions from the U.S. Department of Agriculture, Agricultural Research Service (USDA, ARS) sweetpotato germplasm collection were evaluated for several phenotypic leaf and plant characteristics, and a photographic record of each accession was made. Data were prepared for placement in the USDA, ARS Germplasm Resources Information Network (GRIN) database and the sweetpotato ontology. The parameters recorded for each genotype were canopy coverage, vine length, general leaf outline, leaf lobing, shape of the central leaf lobe, number of leaf points, leaf petiole length, leaf width, leaf length, leaf width × length, and leaf width/length (aspect ratio). The data indicate that there is wide genetic diversity for vegetative phenotypic characteristics within the USDA, ARS sweetpotato germplasm collection. This study provides important phenotype information for the USDA, ARS sweetpotato collection that has been lacking and can be used for curation of the collection and by researchers and breeders working with this important global food crop.

show abstract

Genetic Characterization of Three Egyptian Sweet Potato Genotypes Based on Morpho-Agronomic and Molecular Markers

Cited by 2 publications

References 25 publications

Enhancement of somaclonal variations and genetic diversity using graphite nanoparticles (GtNPs) in sweet potato plants

Enhancement of somaclonal variations and genetic diversity using graphite nanoparticles (GtNPs) in sweet potato plants

Phenotypic Variation in Leaf Morphology of the USDA, ARS Sweetpotato (Ipomoea batatas) Germplasm Collection

Contact Info

Product

Resources

About