Genetic Characterization of the Tick-Borne Orbiviruses

Belaganahalli, Manjunatha N.; Maan, Sushila; Maan, Narender S.; Brownlie, Joe; Tesh, Robert B.; Attoui, Houssam; Mertens, Peter P. C.

doi:10.3390/v7052185

Cited by 39 publications

(37 citation statements)

References 56 publications

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“…The low amino acid identity of LEAV T13 (Table 2) to other T13 proteins confirms that this virus belongs to a distinct serogroup. VP2 is encoded on segment-2 in LEAV and functionally equivalent to VP3 of MBOV and VP4 of TBOV/TOV [46]. Due to its neutralizing epitopes and role in cell attachment, the VP2 (OC1) protein is subjected to intense selective pressures by the host's immune responses.…”

Section: Discussionmentioning

confidence: 99%

Letea Virus: Comparative Genomics and Phylogenetic Analysis of a Novel Reassortant Orbivirus Discovered in Grass Snakes (Natrix natrix)

Tomazatos

Marschang

Maranda

et al. 2020

Viruses

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The discovery and characterization of novel arthropod-borne viruses provide valuable information on their genetic diversity, ecology, evolution and potential to threaten animal or public health. Arbovirus surveillance is not conducted regularly in Romania, being particularly very scarce in the remote and diverse areas like the Danube Delta. Here we describe the detection and genetic characterization of a novel orbivirus (Reoviridae: Orbivirus) designated as Letea virus, which was found in grass snakes (Natrix natrix) during a metagenomic and metatranscriptomic survey conducted between 2014 and 2017. This virus is the first orbivirus discovered in reptiles. Phylogenetic analyses placed Letea virus as a highly divergent species in the Culicoides-/sand fly-borne orbivirus clade. Gene reassortment and intragenic recombination were detected in the majority of the nine Letea virus strains obtained, implying that these mechanisms play important roles in the evolution and diversification of the virus. However, the screening of arthropods, including Culicoides biting midges collected within the same surveillance program, tested negative for Letea virus infection and could not confirm the arthropod vector of the virus. The study provided complete genome sequences for nine Letea virus strains and new information about orbivirus diversity, host range, ecology and evolution. The phylogenetic associations warrant further screening of arthropods, as well as sustained surveillance efforts for elucidation of Letea virus natural cycle and possible implications for animal and human health.

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Section: Discussionmentioning

confidence: 99%

Letea Virus: Comparative Genomics and Phylogenetic Analysis of a Novel Reassortant Orbivirus Discovered in Grass Snakes (Natrix natrix)

Tomazatos

Marschang

Maranda

et al. 2020

Viruses

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“…is the circulation of the viruses between small mammals such as rodents which serve as virus amplifying host. Human is infected after ticks bite as well as consumption of infected milk or milk product [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…Camels are the main hosts of the adults even though they can parasitize other domestic animals [10]. H. dromedarii is a cosmopolitan species since it's widely distributed in North Africa, the northern regions of West, Central, and East Africa, the Far and Middle East as well as South Asia [4,6,11,12]. H. schulzei has shown more limited geographical distribution to Asia and Egypt than H. dromedarii.…”

Section: Introductionmentioning

confidence: 99%

Investigation of hemorrhagic fever viruses inside wild populations of ticks: One of the pioneer studies in Saudi Arabia

Mohamed¹,

Mohamed²,

Aleanizy³

et al. 2017

APJTD

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“…Dengue disease is a public health problem that continues to affect approximately 100 countries globally with an estimated 390 million cases per year of which majority are children. The burden of disease lies mostly in the Asia-Pacific yet the number of affected countries continues to rise as it emerges into new areas putting more people at risk [ 1 ]. Trans-ovarial transmission of dengue virus (DENV) within mosquito populations can facilitate transmission between or within epidemics, thus complicating disease control strategies [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against dengue 2 viral protease

2016

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BackgroundDengue disease is one of the most significant vector-borne illnesses in the world. The emergence and re-emergence of dengue infections in many parts of the world affect millions annually and continue to burden public health systems especially in low-income populations. Advances in dengue vaccine development showed promising results; however, protection seems to be suboptimal. There is no licensed chemotherapeutic agent against dengue to date. An ideal scenario of combinatorial vaccination of high-risk individuals and chemotherapy of the diseased during outbreaks may compensate for the meager protection offered by the vaccine. The dengue virus protease is important to viral replication and, as such, has been identified as a potential target for antivirals. It is, therefore, our objective to establish and optimize an appropriate screening method for use during the early stages of drug development for dengue.MethodsIn this study, we developed and optimized a biochemical assay system for use in screening compound libraries against dengue virus protease. We tested the selected protease inhibitors with a cell-based assay to determine inhibition of viral replication.ResultsWe have presented direct plots of substrate kinetics data showing an apparent inhibition of the protease at excessive substrate concentrations. The most common sources of interference that may have affected the said observation were elucidated. Finally, a screen was done on an existing compound library using the developed method. The compounds selected in this study showed inhibitory activity against both the recombinant dengue protease and cell-based infectivity assays.ConclusionsOur study shows the practicality of a customized biochemical assay to find possible inhibitors of dengue viral protease during the initial stages of drug discovery.Electronic supplementary materialThe online version of this article (doi:10.1186/s41182-016-0025-6) contains supplementary material, which is available to authorized users.

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Genetic Characterization of the Tick-Borne Orbiviruses

Cited by 39 publications

References 56 publications

Letea Virus: Comparative Genomics and Phylogenetic Analysis of a Novel Reassortant Orbivirus Discovered in Grass Snakes (Natrix natrix)

Letea Virus: Comparative Genomics and Phylogenetic Analysis of a Novel Reassortant Orbivirus Discovered in Grass Snakes (Natrix natrix)

Investigation of hemorrhagic fever viruses inside wild populations of ticks: One of the pioneer studies in Saudi Arabia

Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against dengue 2 viral protease

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