Genetic Characterization of Rhizobia Associated with Horse Gram [Macrotyloma uniflorum (Lam.) Verdc.] Based on RAPD and RFLP

Edulamudi, Prabhavati; Johnson, A. M. Anthony; Divi, Venkata Ramana Sai Gopal; Vishnuvardhan, Z.; Konada, Veera Mallaiah

doi:10.9734/bmrj/2015/11457

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…The isolates were clustered on the bases of fingerprinting similarity where few of them had the same origin. Also, our results support the conclusion of De Bruijn, (1992), Edulamudi et al (2015) Evans (2015) and Laguerre et al 1994) that the REP and ERIC PCR could become a powerful tool for the molecular genetic analysis of bacteria and for bacterial taxonomy. It allows the fingerprinting of individual genera, species, and strains and could help to determine phylogenetic relationships.…”

Section: Discussionsupporting

confidence: 90%

“…Several molecular techniques have been readily developed to examine rhizobial biodiversity. These include: RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction), PCR amplification of REP (repetitive extragenic palindrome), ERIC (enterobacterial repetitive intergeneric consensus) and BOX element or RFLP (random amplified length polymorphism) of amplified ribosomal genes (16S, 23S or 16S-23S inter generic spacer -IGS), and nod or nif genes sequencing (De Bruijn, 1992;Edulamudi et al, 2015;Laguerre et al, 1994 andVersalovic et al, 1994). Also, amplified ribosomal DNA restriction analysis (ARDRA) of both 16S and 23S rDNA (Shamseldin et al, 2005) have been used to study the variation that exist for rhizobial isolates.…”

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confidence: 99%

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Biodiversity of Rhizobia That Nodulate Melilotus indicus L. in Egyptian Soils

2015

Egypt. J. Microbiol.

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HE OBJECTIVES of this work were to describe the biodiversity …. and the phylogeny of the selected rhizobial isolates nodulating wild legume Melilotus indicus L. (M. indicus) from 14 different Egyptian soils. These isolates were characterized morphologically and physiologically on the basis of their tolerance to NaCl and pH. Furthermore, the DNA of each rhizobial isolate was analyzed by rep-PCR amplification fingerprinting using REP, ERIC and BOX A1R primers. Thirty seven rhizobial isolates were obtained from the root nodules of M. indicus. These isolates didn`t absorb Congo-red (CR) when incubated in dark; grew poorly, or not at all, on peptone glucose agar medium containing bromocresol purple (BCP) and acidified the medium suggesting fast-growing rhizobia. Five isolates tolerated NaCl up to 7%. Rhizobial isolates showed a wide diversity in their pH tolerance. Moreover, PCR with REP and ERIC primer pairs yielded multiple distinct DNA products for each isolate of size ranged from approximately 177 to 3773 bp and 200 to 2921 bp, respectively. BOX A1R primer did not reveal any polymorphism for the isolates. We can conclude that rhizobia isolated from M. indicus from Egyptian soils are both phenotypically and genetically diverse.

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Section: Discussionsupporting

confidence: 90%

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confidence: 99%