Genetic Characterization of Protostrongylus shiozawai From Japanese Serows (Capricornis crispus)

Pulmonary protostrongyliasis of hare is a parasitic disease caused by nematodes belonging to the genus Protostrongylus (Nematoda, Protostrongylidae). During survey of wildlife disease in the South-East of France, pathologic examination of lungs from European hares found dead or hunter-killed between 2009 and 2012 was performed. Adult male worms were morphologically characterized and the identification confirmed by molecular biology (D2 domain of the 28S and ITS2 of rDNA). Two different species were identified: the first one, Protostrongylus pulmonalis, is identical with the haplotype previously deposited in GenBank. Based on morphological criteria of copulatory bursa of adult male worms (especially length of spicules and gubernaculum structure), we identified a second species found in France as Protostrongylus oryctolagi. This is the first report of P. oryctolagi in France from European hare and rabbit. P. oryctolagi was isolated from 248 hares and 3 rabbits in the South of France. P. pulmonalis was isolated from four hares found dead in the Northern France and from one hare in the South, which was co-parasitized by P. oryctolagi and P. pulmonalis. It's the first coinfection observed with these two species from a lung of hare in France.

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Protostrongylus pulmonalis (Frölich, 1802) and P. oryctolagi Baboš, 1955 (Nematoda: Protostrongylidae), parasites of the lungs of European hare (Lepus europaeus L.) in France: morphological and molecular approaches

Lesage

Jouet

Patrelle

et al. 2014

Parasitol Res

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Molecular phylogeny and diagnosis of species of the family Protostrongylidae from caprine hosts in Uzbekistan

et al. 2015

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Protostrongylids are important pulmonary parasites of ungulates, particularly caprines. Their complex life cycles involve terrestrian gastropods as intermediate hosts. Morphological discrimination of larvae in feces and snails is impossible to the species level, and molecular data are missing for many species. To improve diagnosis and epidemiology of protostrongylids, this study describes internal transcribed spacer 2 (ITS-2) sequences for five protostrongylid and one metastrongylid species from Uzbekistan. Morphological and molecular analyses identified Protostrongylus rufescens, Protostrongylus hobmaieri, Protostrongylus sp., Spiculocaulus leuckarti, and Cystocaulus ocreatus. Sequence differences between ITS-2 were sufficient to allow species identification, e.g., in the Protostrongylinae intraspecific and interspecific genetic distances ranged between 0-0.01 and 0.05 and 0.16, respectively. There was one exception in the Elaphostrongylidae with identical ITS-2 sequences in Elaphostrongylus cervi and Elaphostrongylus rangiferi questioning their status as valid species. Maximum likelihood analysis sequences largely supported the currently assumed phylogenetic relationships among Protostrongylidae as predicted using morphological characters. The monophyly of the subfamilies Varestrongylinae, Elaphostrongylinae, and Protostrongylinae was corroborated with support values in Shimodaira-Hasegawa or Bayesian modifications of the approximate likelihood ratio test (aLRT) ≥97%, but support for Muellerinae was weak (8 and 52%, respectively) since Muellerius capillaris differs significantly from the other Muellerinae. On the genus level, paraphyly of the genus Protostrongylus was confirmed since the members of the genera Spiculocaulus and Orthostrongylus were located within the Protostrongylus cluster (aLRT ≥99%). Maximum likelihood unequivocally assigned every unique sequence to the correct species confirming suitability of ITS-2 regions for diagnosis protostrongylids except of E. cervi and E. rangiferi.

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Genetic Characterization of Protostrongylus shiozawai From Japanese Serows (Capricornis crispus)

Cited by 2 publications

References 18 publications

Protostrongylus pulmonalis (Frölich, 1802) and P. oryctolagi Baboš, 1955 (Nematoda: Protostrongylidae), parasites of the lungs of European hare (Lepus europaeus L.) in France: morphological and molecular approaches

Protostrongylus pulmonalis (Frölich, 1802) and P. oryctolagi Baboš, 1955 (Nematoda: Protostrongylidae), parasites of the lungs of European hare (Lepus europaeus L.) in France: morphological and molecular approaches

Molecular phylogeny and diagnosis of species of the family Protostrongylidae from caprine hosts in Uzbekistan

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