Genetic characterization of poxviruses in Camelus dromedarius in Ethiopia, 2011–2014

Gelaye, Esayas; Achenbach, Jenna E.; Ayelet, Gelagay; Jenberie, Shiferaw; Yami, Martha; Grabherr, Reingard; Loitsch, Angelika; Diallo, Adama; Lamien, Charles Euloge

doi:10.1016/j.antiviral.2016.08.016

Cited by 11 publications

(9 citation statements)

References 25 publications

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“…Clustering of various CMLV isolates in three distinct clusters from various regions of the globe may indicate migration and/or circulation of different CMLV strains. CMLV‐Syria, a strain from Middle‐East showed clustering with African CMLV strains (Ethiopian and Somalian CMLV strains) as reported earlier also (Gelaye et al., ). CMLV strains showed more genetic relatedness to VARV and TATV than to other OPVs as expected and reported (Balamurugan et al., , ).…”

Section: Discussionsupporting

confidence: 80%

“…On analysis, a deletion of seven nucleotides was found at C‐terminus in Indian CMLV strains, similar to CMLV‐M96. This deletion caused a frameshift in amino acid sequence resulting into translated proteins of 319 aa length, whereas Middle‐East CMLV strains and African CMLV strains translate HA proteins of 315 aa length due to stop codon as reported earlier (Gelaye et al., ). HA gene of CMLV‐Bik06 strain from India reported previously (Nagarajan et al., ) also showed similar deletion, but it was partial sequence due to designing of primers primarily based on CMLV‐Syria as reported.…”

Section: Discussionsupporting

confidence: 55%

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Sequence analysis of haemagglutinin gene of camelpox viruses shows deletion leading to frameshift: Circulation of diverse clusters among camelpox viruses

Venkatesan

Kumar

Prabhu

et al. 2018

Transbound Emerg Dis

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Orthopoxviruses (OPVs) have broad host range infecting a variety of species along with gene-specific determinants. Several genes including haemagglutinin (HA) are used for differentiation of OPVs. Among poxviruses, OPVs are sole members encoding HA protein as part of extracellular enveloped virion membrane. Camelpox virus (CMLV) causes an important contagious disease affecting mainly young camels, endemic to Indian subcontinent, Africa and the Middle East. This study describes the sequence features and phylogenetic analysis of HA gene (homologue of VACV A56R) of Indian CMLV isolates. Comparative analysis of CMLV HA gene revealed conserved nature within CMLVs but considerable variability was observed between various species of OPVs. Most Indian CMLV isolates showed 99.5%-100% and 96.3%-100% identity, at nucleotide (nt) and amino acid (aa) levels respectively, among themselves and with CMLV-M96 strain. Importantly, Indian CMLV strains along with CMLV-M96 showed deletion of seven nucleotides resulting in frameshift mutation at C-terminus of HA protein. Phylogenetic analysis displayed distinct clustering among CMLVs which might point to the circulation of diverse CMLV strains in nature. Despite different host specificity of OPVs, comparative sequence analysis of HA protein showed highly conserved N-terminal Ig V-set functional domain with tandem repeats. Understanding of molecular diversity of CMLVs and structural domains of HA protein will help in the elucidation of molecular mechanisms for immune evasion and design of novel antivirals for OPVs.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 55%

Sequence analysis of haemagglutinin gene of camelpox viruses shows deletion leading to frameshift: Circulation of diverse clusters among camelpox viruses

Venkatesan

Kumar

Prabhu

et al. 2018

Transbound Emerg Dis

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“…Based on a previous study, CCEV is most likely a subclade of PCPV which has adapted to camels as they are genetically closely related 17 . Our sequence analysis data showed all the isolates of CCEV in this study clustered closely with PCPV 45 , thus, we considered isolates of CCEV genotyped as PCPV.…”

Section: Methodsmentioning

confidence: 76%

A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance

Gelaye

Mach

Kolodziejek

et al. 2017

Sci Rep

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Poxviruses belonging to the Orthopoxvirus, Capripoxvirus and Parapoxvirus genera share common host species and create a challenge for diagnosis. Here, we developed a novel multiplex PCR method for the simultaneous detection and differentiation of eight poxviruses, belonging to three genera: cowpox virus (CPXV) and camelpox virus (CMLV) [genus Orthopoxvirus]; goatpox virus (GTPV), sheeppox virus (SPPV) and lumpy skin disease virus (LSDV) [genus Capripoxvirus]; orf virus (ORFV), pseudocowpox virus (PCPV) and bovine papular stomatitis virus (BPSV) [genus Parapoxvirus]. The assay is based on high-resolution melting curve analysis (HRMCA) of PCR amplicons produced using genus specific primer pairs and dsDNA binding dye. Differences in fragment size and GC content were used as discriminating power. The assay generated three well separated melting regions for each genus and provided additional intra-genus genotyping allowing the differentiation of the eight poxviruses based on amplicon melting temperature. Out of 271 poxviral DNA samples tested: seven CPXV, 25 CMLV, 42 GTPV, 20 SPPV, 120 LSDV, 33 ORFV, 20 PCPV and two BPSV were detected; two samples presented co-infection with CMLV and PCPV. The assay provides a rapid, sensitive, specific and cost-effective method for the detection of pox diseases in a broad range of animal species and humans.

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“…Recent studies published after the completion of this manuscript compared a large number of samples using the HA and B2L genes [ 28 ] and the C18L gene [ 13 ], which were not studied here. The completion of the full genome sequence of the newly isolated subtype (work in progress) will allow for a better comparison, and will also determine its similarity to other studied isolates.…”

Section: Discussionmentioning

confidence: 99%

First Diagnosed Case of Camelpox Virus in Israel

Erster¹,

Melamed

Paran

et al. 2018

Viruses

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An outbreak of a disease in camels with skin lesions was reported in Israel during 2016. To identify the etiological agent of this illness, we employed a multidisciplinary diagnostic approach. Transmission electron microscopy (TEM) analysis of lesion material revealed the presence of an orthopox-like virus, based on its characteristic brick shape. The virus from the skin lesions successfully infected chorioallantoic membranes and induced cytopathic effect in Vero cells, which were subsequently positively stained by an orthopox-specific antibody. The definite identification of the virus was accomplished by two independent qPCR, one of which was developed in this study, followed by sequencing of several regions of the viral genome. The qPCR and sequencing results confirmed the presence of camelpox virus (CMLV), and indicated that it is different from the previously annotated CMLV sequence available from GenBank. This is the first reported case of CMLV in Israel, and the first description of the isolated CMLV subtype.

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Genetic characterization of poxviruses in Camelus dromedarius in Ethiopia, 2011–2014

Cited by 11 publications

References 25 publications

Sequence analysis of haemagglutinin gene of camelpox viruses shows deletion leading to frameshift: Circulation of diverse clusters among camelpox viruses

Sequence analysis of haemagglutinin gene of camelpox viruses shows deletion leading to frameshift: Circulation of diverse clusters among camelpox viruses

A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance

First Diagnosed Case of Camelpox Virus in Israel

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