Genetic Characterization of Lactic Acid Bacteria Isolated from Tunisian Milk Waste and their Antimicrobial Activity Against some Bacteria Implicated in Nosocomial Infections

Ghodhbane, Hanen; Alessandria, Valentina; Snoussi, Mejdi; Elleuch, Lobna; Trabelsi, Ismaîl; Abdelly, Chédly; Sabatier, J.-M.; Cocolin, Luca Simone; Regaya, Imed

doi:10.2174/1871526516666160719160150

Cited by 4 publications

(2 citation statements)

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“…The traditional classification of LAB is mainly based on morphological observation and biochemical experiments (Cai et al, 1999;Makela et al, 1992;Shaw et al, 1985). With the development of molecular biology techniques, genotyping methods such as microbial genome sequencing, 16S rDNA sequence analysis, polymerase chain reaction (PCR) fingerprinting, and DNA hybridization technology have been widely used (Domingos-Lopes et al, 2017;Ghodhbane et al, 2016;Ramos et al, 2018;Zhao et al, 2015). However, the genotype and phenotype of some strains are inconsistent, resulting in different classification results.…”

Section: Probioticsmentioning

confidence: 99%

Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

Meng¹,

LI²,

YANG³

et al. 2023

Biocell

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In recent years, microbial metabolomics, a new field that has attracted wide attention, provides a map of metabolic pathways and clarifies the interaction mechanism between microorganisms and hosts. Many microorganisms are found in the human intestine, oral cavity, vagina, etc. Probiotics could maintain the good health of the host, while pathogens and an imbalance of bacterial flora lead to a series of diseases of the body and mind.Metabolomics is a science for qualitative and quantitative analysis of all metabolites in an organism or biological system, which could provide key information to understand the related metabolic pathways and associated changes. This approach analyzes the final products of cellular regulatory processes, the level of which can be regarded as the ultimate response of the biological system to genetic or environmental changes. Microbial metabolomics has been widely used in different research fields, such as microbial phenotypic classification, mutant screening, metabolic pathways, microbial metabolic engineering, fermentation engineering monitoring and optimization, microbial environmental pollution, and so on. However, there are only a few reviews on microbial metabolomics of probiotics and pathogens. This review summarizes the main methodologies, including sample preparation, identification of metabolites, data processing, and analysis. Recent applications in microbial metabolomics of probiotics and pathogens are also described. This paper first summarized the research progress and application of microbial metabolomics from two aspects: probiotics and pathogenic bacteria. Probiotics and pathogenic bacteria do not exist independently most of the time; hence, these were reviewed in the research field of coexistence of probiotics and pathogenic bacteria, which was subdivided into important microbial research fields closely related to human health, including the human gut, oral cavity, food, and nutrition-related microorganisms. Then, the main problems and trends associated with microbial metabolomics are discussed.

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Section: Probioticsmentioning

confidence: 99%

Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

Meng¹,

LI²,

YANG³

et al. 2023

Biocell

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show abstract

“…Recently, it has become worthwhile research to turn to the strategic environmental challenge for effective treatment and advanced valorization technologies of different wastes generated from the entire dairy chain into economic incentives. In this context and from an economic point of view, recent studies have highlighted the potential reuse of dairy wastes for nutritive components production (Kasmi et al 2017a), isolation and selection of lactic acid bacteria for their antimicrobial activities against different pathogenic bacteria causing nosocomial infections (Ghodhbane et al 2016), and low-cost lactic acid bacteria growth media production (Kasmi et al 2018).…”

Section: Introductionmentioning

confidence: 99%

A new practical approach for the biological treatment of a mixture of cheese whey and white wastewaters using Kefir grains

Elleuch

Salem-Berrabah

Cherni

et al. 2020

Environ Sci Pollut Res

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Kefir grains are a microbial consortium of different genera of bacteria and yeasts. In this study, the performance of Tunisian Kefir grains during the biological treatment of a mixture of Gouda cheese whey and white wastewaters (GCW) in ratio 1:1 with very high organic matter concentration is investigated. The biological 2 process was evaluated and optimized through the response surface methodology. Under the optimum conditions, Kefir grains concentration of 1.02%, temperature at 36.68 °C, and incubation time of 5.14 days, the removal efficiencies of COD, PO4 3− , and NO3 − were 87, 37.48, and 39.5%, respectively.Interestingly, the reusability tests of the grains proved not only their high resistance to harsh environmental conditions but also their great potential for more practical applications. Particularly, different strains were isolated from the grains and identified as Kluyveromyces marxianus, Lactoccocus lactis, Lactobacillus kefiri, and Bacillus spp. using 16S rDNA sequence analysis and rep-PCR fingerprinting. At the biological level, the raw GCW (RGCW) has a negative impact on the Hordeum vulgare both on seed germination, and on the growth parameters of seedlings. Interestingly, after Kefir grains treatment, the treated GCW (TGCW) allow a seedlings growth and germination rate similar to those soaked in water.

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Organic Compounds and Heavy Metals Simultaneous Removal from a Tunisian Landfill Leachate Using Dairy Rejects

Cherni

Kasmi

Elleuch

et al. 2018

Advances in Science, Technology &Amp; Innovation

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Genetic Characterization of Lactic Acid Bacteria Isolated from Tunisian Milk Waste and their Antimicrobial Activity Against some Bacteria Implicated in Nosocomial Infections

Abstract: All L. lactis bacteriocin producing strains possessed the Nisine Z gene (nisZ) except for one, which contained both Nisine A and Nisine Z genes (nisA and nisZ). They have been identified as antilisterial agentS.

Cited by 4 publications

References 0 publications

Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

A new practical approach for the biological treatment of a mixture of cheese whey and white wastewaters using Kefir grains

Organic Compounds and Heavy Metals Simultaneous Removal from a Tunisian Landfill Leachate Using Dairy Rejects

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