Genetic characterization of hepatitis A virus isolates from Buenos Aires, Argentina

Mbayed, Viviana Andrea; Sookoian, Silvia; Alfonso, Victoria; Campos, Rodolfo Héctor

doi:10.1002/jmv.10194

Cited by 31 publications

(44 citation statements)

References 13 publications

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“…Phylogenetic analysis of the nucleotide sequence of the VP1/2A region of the HAV genome classified all isolates as genotype I, confirming former epidemiological data demonstrating that this is the main genotype circulating in Brazil (7,10,22) as well the most widespread genotype in the world (2,23,24). Additionally, we identified the simultaneous presence of sub-genotypes IA and IB in 65 and 35% of cases of HAV infection, respectively.…”

Section: Discussionsupporting

confidence: 86%

Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil

Villar

Morais

Aloise

et al. 2006

Braz J Med Biol Res

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The Northeast region is the location of most cases of acute hepatitis A virus (HAV) in Brazil. In the present study, the genotypes of HAV strains from Pernambuco State, one of most populous states in the Northeast region, were characterized. Blood samples positive for anti-HAV IgM from 145 individuals (mean age = 29.1 years), collected during 2002 and 2003, were submitted to nested RT-PCR for amplification of the 5'non-translated region (5'NTR) and VP1/2A regions of the HAV genome. The VP1/2A and 5'NTR regions were amplified in 39 and 21% of the samples, respectively. Nucleotide sequencing was carried out in 46% of VP1/2A and in 53% of 5'NTR isolates. The identity in nucleotide sequence of the VP1/2A region ranged from 93.6 to 100.0%. Phylogenetic analysis of the VP1/2A sequences showed that 65% belong to sub-genotype IA and 35% to sub-genotype IB. Co-circulation of both sub-genotypes was observed in the two years studied. Distinct clusters of highly related sequences were observed in both sub-genotypes, suggesting endemic circulation of HAV strains in this area. In the 5'NTR isolates, 92.7-99.2% identity was observed and two isolates presented one deletion at position 413. Phylogenetic analysis showed that genotype IA strains cluster in the tree in the same way as genotype IB strains, but one IIIA isolate from Spain clusters with genotype IB strains. These results do not allow us to state that 5'NTR could be used to genotype HAV sequences. This is the first report of co-circulation of sub-genotypes IA and IB in this region, providing additional information about the molecular epidemiology of HAV strains in Brazil.

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Section: Discussionsupporting

confidence: 86%

Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil

Villar

Morais

Aloise

et al. 2006

Braz J Med Biol Res

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“…The prevalence of sub-group IA in the tested samples is in line with our previous reports [13], in which sub-genotype IA was the predominant type in acute HAV infection. This sub-genotype was predominant in other countries such as Brazil, Cuba, Costa Rica, Argentina, and Thailand [2,10,11,20,23]. Other reports described the prevalence of sub-genotype IB Turkey [22].…”

Section: Discussionmentioning

confidence: 88%

Seroprevalence and Molecular Characterisation of Human Hepatitis A virus in Serum Samples of Tunisian Patients with Clinical Symptoms of Viral Hepatitis

et al. 2012

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The aim of the present study was to investigate the seroprevalence of Hepatitis A virus antibodies in patients with clinical symptoms of viral hepatitis and molecular characterization of the detected isolates. The present study deals with the seroprevalence and the genetic diversity of HAV in 400 Tunisian patients presenting in dispensaries (160 patients) and in University Hospitals (240 patients) with hepatitis symptoms between 2006 and 2008. The patients with acute hepatitis were mainly from rural regions. However, the total number of patients was decreased over time. The collected samples were from patients with hepatitis symptoms occurring mainly during January-March (36.7, 26, and 35.5%) and SeptemberDecember (39.4, 43.4,and 35.5%) during the three years of study, respectively. However, HAV infection was established for only 110 among 400 patients. The detected isolates were clustered within sub-genotype IA. The present study constituted another report of the continued surveillance of HAV infection in the region of Monastir and the molecular characterisation of the detected strains.

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“…Most of the human isolates have been found to belong to genotype I, in which subgenotype IA predominates over subgenotype IB. Previous studies have reported the occurrence of HAV subgenotype IA in Argentina, Brazil, Chile, Costa Rica, Cuba, El Salvador, and Uruguay [Robertson et al, 1992;Stene-Johansen et al, 1998;Arauz-Ruiz et al, 2001;Costa-Mattioli et al, 2001a;Diaz et al, 2001;Mbayed et al, 2002]. It has thus been suggested that the population of HAV circulating endemically in South America belongs to one subgenotype (IA) [CostaMattioli et al, 2001a].…”

Section: Discussionmentioning

confidence: 96%

Genetic analysis of hepatitis A virus isolates from Brazil

Paula

Lü

Niel

et al. 2004

Journal of Medical Virology

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A limited number of hepatitis A virus (HAV) isolates from South America have been characterised at the genomic level. IgM anti-HAV positive serum samples collected from patients with hepatitis A living in the five geographical regions of Brazil (North, Northeast, Central, South, and Southeast) were used to obtain HAV isolates and determine their genetic relatedness. Of the 232 case isolates, sequence data were obtained from the VP1/2A junction region of the HAV genome. All isolates were classified in genotype I; 231 belonged to subgenotype IA, and one to subgenotype IB. HAV isolates from four States formed distinct clusters of highly related sequences. However, isolates from other states did not cluster and the sequences from those states were intermingled with sequences found in the other states. The amino acid sequences of all but two isolates showed a Leu --> Ile substitution at position 42 in the 2A protein. This substitution appeared to be a characteristic geographic fingerprint of HAV sequences within Brazil.

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Genetic characterization of hepatitis A virus isolates from Buenos Aires, Argentina

Cited by 31 publications

References 13 publications

Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil

Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil

Seroprevalence and Molecular Characterisation of Human Hepatitis A virus in Serum Samples of Tunisian Patients with Clinical Symptoms of Viral Hepatitis

Genetic analysis of hepatitis A virus isolates from Brazil

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