Genetic characterization of group A acetylsaponin‐deficient mutants from wild soybean (<i>Glycine soja</i> Sieb. and Zucc.)

Krishnamurthy, Panneerselvam; Lee, Jeong‐Dong; Ha, Bo‐Keun; Chae, Jong-Hyun; Song, Jong Tae; Tsukamoto, Chigen; Singh, R. J.; Chung, Gyuhwa

doi:10.1111/pbr.12269

Cited by 8 publications

(6 citation statements)

References 28 publications

(79 reference statements)

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“…Soybean is one of the most important oilseed crops worldwide, which is rich in vegetable oil, protein and nutraceutical compounds such as isoflavones and saponins (Lam et al, 2010; Schmutz et al, 2010; Korir et al, 2013; Krishnamurthy et al, 2015). It is widely used for human food, animal feed, and industrial products.…”

Section: Introductionmentioning

confidence: 99%

Identification and Analysis of NaHCO3 Stress Responsive Genes in Wild Soybean (Glycine soja) Roots by RNA-seq

et al. 2016

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Soil alkalinity is a major abiotic constraint to crop productivity and quality. Wild soybean (Glycine soja) is considered to be more stress-tolerant than cultivated soybean (G. max), and has considerable genetic variation for increasing alkalinity tolerance of soybean. In this study, we analyzed the transcriptome profile in the roots of an alkalinity tolerant wild soybean variety N24852 at 12 and 24 h after 90 mM NaHCO3 stress by RNA-sequencing. Compared with the controls, a total of 449 differentially expressed genes (DEGs) were identified, including 95 and 140 up-regulated genes, and 108 and 135 down-regulated genes at 12 and 24 h after NaHCO3 treatment, respectively. Quantitative RT-PCR analysis of 14 DEGs showed a high consistency with their expression profiles by RNA-sequencing. Gene Ontology (GO) terms related to transcription factors and transporters were significantly enriched in the up-regulated genes at 12 and 24 h after NaHCO3 stress, respectively. Nuclear factor Y subunit A transcription factors were enriched at 12 h after NaHCO3 stress, and high percentages of basic helix-loop-helix, ethylene-responsive factor, Trihelix, and zinc finger (C2H2, C3H) transcription factors were found at both 12 and 24 h after NaHCO3 stress. Genes related to ion transporters such as ABC transporter, aluminum activated malate transporter, glutamate receptor, nitrate transporter/proton dependent oligopeptide family, and S-type anion channel were enriched in up-regulated DEGs at 24 h after NaHCO3 treatment, implying their roles in maintaining ion homeostasis in soybean roots under alkalinity. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed “phenylpropanoid biosynthesis” and “phenylalanine metabolism” pathways might participate in soybean response to alkalinity. This study provides a foundation to further investigate the functions of NaHCO3 stress-responsive genes and the molecular basis of soybean tolerance to alkalinity.

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Section: Introductionmentioning

confidence: 99%

Identification and Analysis of NaHCO3 Stress Responsive Genes in Wild Soybean (Glycine soja) Roots by RNA-seq

et al. 2016

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“…The deduced molecular structures of these new components are shown in Fig. 6 Confirmation of a chemical reaction required for arabinose binding at the C-22 position in the biosynthesis of group A saponins Many loci and genes or enzymes involved in sugar chains in the biosynthesis of soybean saponins have been reported (Kikuchi et al 1999, Shibuya et al 2010, Krishnamurthy et al 2015. However, the integrated biosynthesis of saponins is only partially known, particularly three steps: DDMP and arabinose binding to the C-22 hydroxyl group in DDMP and group A saponins, respectively, and the acetylation of the terminal sugar at the C-22 sugar chain in group A saponins.…”

Section: Discussionmentioning

confidence: 71%

“…, Krishnamurthy et al. ). However, the integrated biosynthesis of saponins is only partially known, particularly three steps: DDMP and arabinose binding to the C‐22 hydroxyl group in DDMP and group A saponins, respectively, and the acetylation of the terminal sugar at the C‐22 sugar chain in group A saponins.…”

Section: Discussionmentioning

confidence: 99%

Identification of a novel variant lacking group A soyasaponin in a Chinese wild soybean (Glycine soja Sieb. & Zucc.): implications for breeding significance

Takahashi

Tsukamoto

et al. 2016

Plant Breeding

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Group A saponins are the principal factors underlying the undesirable bitter and astringent tastes in soybean food products. Therefore, the genetic reduction or elimination of the undesirable tastes is of great significance in soybean taste breeding. Using thin‐layer chromatography and liquid chromatography–mass spectrometry, we identified a novel mutant lacking group A saponins in a wild soybean (Glycine soja Sieb. & Zucc.), reflecting the failed arabinose binding of arabinosyltransferase to the C‐22 hydroxyl group, consequently leading to a lack of group A saponins and the concomitance expression of two new components A‐αg and KA‐αg in seeds. The C‐3 glycosylated soyasapogenol A (SS‐A), A‐αg, is a steady precursor compound in biosynthesis of Aa and Ab types in the seeds of soybean plants and exhibits stable inheritance. In this study, we observed a critical step necessary for arabinose binding to the C‐22 hydroxyl group in the biosynthetic pathway of group A saponins and identified an important germplasm accession for the genetic improvement in the tastes of soybean milk and processed soybean foods.

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“…Recently, 12 mutants with a deficiency of group A acetylsaponins were identified from the Korean wild soybean collection (Krishnamurthy et al, 2014a; Panneerselvam et al, 2013). The mutants carried a single point mutation at the +948 position (G→A change) of the Sg‐1 a gene and that point mutation was stably inherited by a single recessive allele ( sg‐1 0‐a1 ) (Krishnamurthy et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a New Allele of the Saponin‐Synthesizing Gene Sg‐1 in Soybean

et al. 2016

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Saponins are major components of secondary metabolites in soybean [Glycine max (L.) Merr.] seed that have been broadly studied for their beneficial effects on human health. In soybean, group A acetylsaponins can be classified into two types: Aa‐ and Ab‐series, both of which cause bitter and astringent aftertaste. Therefore, group A acetylsaponin‐deficient cultivars can solve a problem that has inhibited the broader use of soybean in the food industry. The objective of this study was to investigate a group A acetylsaponin‐deficient soybean mutant (PE1515) generated from the cultivar Pungsannamul (containing Ab‐series saponins) by ethyl methanesulfonate treatment. The phenotypic and genotypic variation was investigated for the mutant PE1515. Our analyses showed that in soybean line PE1515, the Ab‐series saponins were absent and A0‐αg saponin accumulated as the major saponin. PE1515 had a single base substitution from G to A at 1004 bp of the Sg‐1 gene, which introduced a premature stop codon in the Sg‐1 gene. A DNA marker to detect the single nucleotide substitution was developed for breeding purposes. Based on this genetic marker, the association of the sg‐1 allele in PE1515 was tested using 120 F2 progeny of a cross Pungsannamul × PE1515. The results showed that the mutation of the Sg‐1 gene in PE1515 contributed to the lack of Ab‐series saponins in the seed hypocotyl. The soybean line PE1515, which does not produce detectable Ab‐series saponins, would be a good genetic resource to improve soybean for wider use in foods.

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Genetic characterization of group A acetylsaponin‐deficient mutants from wild soybean (Glycine soja Sieb. and Zucc.)

Cited by 8 publications

References 28 publications

Identification and Analysis of NaHCO3 Stress Responsive Genes in Wild Soybean (Glycine soja) Roots by RNA-seq

Identification and Analysis of NaHCO3 Stress Responsive Genes in Wild Soybean (Glycine soja) Roots by RNA-seq

Identification of a novel variant lacking group A soyasaponin in a Chinese wild soybean (Glycine soja Sieb. & Zucc.): implications for breeding significance

Characterization of a New Allele of the Saponin‐Synthesizing Gene Sg‐1 in Soybean

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