Genetic characterization of four dog breeds with Illumina CanineHD BeadChip

Yang, Zihao; Zhang, Jingyi; Tao, Ruiyang; Ren, Wei; Zhang, Jie; Dong, Jun; Li, Chengtao; Zhang, Suhua

doi:10.1080/20961790.2019.1614292

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…In addition, the implementation of high-throughput DNA-genotyping arrays has led to the generation of SNP genotypes of different domestic and wild animal species, which might be used as reliable comparison groups for taxonomic, species, and breed/ecotype assignment of the tested samples, without further validation. Thus, Yang Z et al [ 33 ] demonstrated the utility of the Illumina Canine HD BeadChip for dog breed recognition, which is crucial in domestic animal forensic genetics.…”

Section: Discussionmentioning

confidence: 99%

Combination of Multiple Microsatellite Analysis and Genome-Wide SNP Genotyping Helps to Solve Wildlife Crime: A Case Study of Poaching of a Caucasian tur (Capra caucasica) in Russian Mountain National Park

Rodionov

Deniskova

Dotsev

et al. 2021

Animals

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Poaching is one of the major types of wildlife crime in Russia. Remnants of goats (presumably the wild endemic species, the Caucasian tur) were found in an area of the Caucasian mountains. The case study involves a suspected poacher whose vehicle was found to have two duffel bags containing pieces of a carcass, which he claimed was that of a goat from his flock. The aim of the forensic genetic analysis for this case was to (i) establish individual identity and (ii) perform species identification. DNA typing based on fourteen microsatellites revealed that STR-genotypes generated from pieces of evidence found at crime scene fully matched those obtained from the evidence seized from the suspect. The results of genome-wide SNP-genotyping, using Illumina Goat SNP50 BeadChip, provided evidence that the poached animal was a wild Caucasian tur (Capra caucasica). Thus, based on comprehensive molecular genetic analysis, evidence of poaching was obtained and sent to local authorities. To our knowledge, this case study is the first to attempt to use DNA chips in wildlife forensics of ungulates.

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Section: Discussionmentioning

confidence: 99%

Combination of Multiple Microsatellite Analysis and Genome-Wide SNP Genotyping Helps to Solve Wildlife Crime: A Case Study of Poaching of a Caucasian tur (Capra caucasica) in Russian Mountain National Park

Rodionov

Deniskova

Dotsev

et al. 2021

Animals

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Genetic Polymorphism and Relationship Analyses of Standard Poodle and Bichon Frise Groups Based on 19 Short Tandem Repeat Loci

Mei,

Yang,

et al. 2023

Journal of Forensic Science and Medicine

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Context: As the increasing number of pet canines, the identification of canine has attracted much attentions in the forensic field, however, the genetic diversities of pet canines still remained unknown. Aims: To explore genetic polymorphisms of 19 short tandem repeat (STR) loci and genetic relationships between the two studied canine groups and reference group. Subjects and Methods: In the present study, genetic polymorphisms of 19 STR loci and a sex-linked zinc finger locus were analyzed in a total of 594 canines in Standard Poodle and Bichon Frise groups from China. Results: A total of 166, 159 alleles were observed in the Standard Poodle, Bichon Frise groups with the corresponding allelic frequencies ranging from 0.0030–0.6108 to 0.0012–0.6148, respectively. The combined discrimination power and probability of exclusion of 19 STR loci in Standard Poodle and Bichon Frise groups were 0.9999999999999497, 0.999962884; and 0.99999999999999995, 0.999965955, respectively. Furthermore, the genetic distances between the two canine groups and Labrador retriever group were calculated, and the results indicated that Standard Poodle and Bichon Frise groups showed a closer genetic relationship, while the two canine groups had distant genetic relationships with Labrador retriever group. The result of population genetic structure revealed that genetic component distributions in the three canine groups were different. The predicted accuracies of the constructed random forest prediction model for three validation sets (25% individuals randomly selected from three populations with 808 individuals) were higher than 0.9, especially for the individuals in validation set from the Bichon Frise group is 1. Conclusions: The 19 STR loci could be used for individual identification, canine breed identification and paternity testing in the two canine groups.

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Genetic characterization of four dog breeds with Illumina CanineHD BeadChip

Cited by 2 publications

References 15 publications

Combination of Multiple Microsatellite Analysis and Genome-Wide SNP Genotyping Helps to Solve Wildlife Crime: A Case Study of Poaching of a Caucasian tur (Capra caucasica) in Russian Mountain National Park

Combination of Multiple Microsatellite Analysis and Genome-Wide SNP Genotyping Helps to Solve Wildlife Crime: A Case Study of Poaching of a Caucasian tur (Capra caucasica) in Russian Mountain National Park

Genetic Polymorphism and Relationship Analyses of Standard Poodle and Bichon Frise Groups Based on 19 Short Tandem Repeat Loci

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