Genetic characterization of dengue virus serotype 2 isolated from dengue fever outbreaks in eastern Uttar Pradesh and western Bihar, India

Abstract

Dengue (DEN) is the most common cause of mosquito‐borne endemic viral diseases in the tropical and subtropical countries. DEN outbreaks associated with multiple dengue virus (DV) serotypes have been regularly reported in different parts of India. This study was done during DEN outbreaks in 2015 to 2016 in UP and Bihar where DEN‐2 was found as the only prevalent serotype. DV‐2 was the only serotype amplified in serotype‐specific reverse‐transcription polymerase chain reaction from sera of 210 (65.21%) out of 322 DV NS1 antigen‐positive patients. Further genetic analysis based on full‐length envelope (E) protein sequence derived from patient's sera as well as DV isolate showed the circulation of lineages I and III of DV‐2 cosmopolitan genotype during 2015 and lineage II during 2016. Finally, the phylogenetic analysis using the E gene sequence revealed that these DV‐2 strains have a close genetic relationship with the recently reported DV‐2 genotypes from DEN outbreaks reported from different parts of north India. These results showed the circulation of cosmopolitan genotype of DV‐2 in eastern Uttar Pradesh and western Bihar, India. The genetic database generated on circulating DV strains in this study will be useful as reference for disease surveillance and strengthening laboratory diagnosis protocols.

Dengue Burden and Circulation of Dengue-2 Serotype Among Children Along With Clinical Profiling in Uttarakhand, India: A Cross-Sectional Study From 2018 to 2020

“…[19]. Deval et al from Uttar Pradesh reported the DEN-2 serotype in the population[20]. These states are near Uttarakhand and many locals travel in this state regularly which gives support to the theory of serotypes difference due to the travellers from different parts of the country.…”

Design of an Epitope-Based Peptide Vaccine Against Dengue Virus Isolate from Eastern Uttar Pradesh, India

“…Envelope protein (Protein Id: AWI48553.1) of Dengue virus 2 isolate NIV/GFU-DV-1615135 was downloaded from the protein database of NCBI. This strain was discovered in serum samples taken clinically confirmed the dengue fever in patients who visited from 2015 to 2016 in Gorakhnath Multispecialty Hospital, Gorakhpur (Deval et al 2021 ). E protein of DENV-2 was amplified from the virus in NIV, Gorakhpur, UP (Deval et al 2021 ).…”

“…This strain was discovered in serum samples taken clinically confirmed the dengue fever in patients who visited from 2015 to 2016 in Gorakhnath Multispecialty Hospital, Gorakhpur (Deval et al 2021 ). E protein of DENV-2 was amplified from the virus in NIV, Gorakhpur, UP (Deval et al 2021 ). E protein gene sequences from internationally scattered DENV-2 serotypes share 96–100% sequence similarity at the nucleotide level.…”

“…The DENV2could be responsible for the recent deaths in the western UP districts of Aligarh, Mathura, Agra, and Firozabad due to a strange fever. Dengue fever has been detected in more than 15 states throughout the country (Deval et al 2021 ). Dengue fever cases have been progressively increasing across the country, posing significant hurdles for medical personnel.…”

Investigating the Clinico-Demographic Characteristics of Dengue Fever and Its Seroprevalence at a Tertiary Care Hospital in Northern India

“…India also continues to be a prominent location for dengue cases worldwide [ 2 , 5 ]. The epidemiology of dengue fever is undergoing significant developments, with more extensive and frequent outbreaks being recorded in urban and rural regions, attributable to each of the four serotypes in India [ 6 ]. Efforts are underway to improve dengue surveillance in India, but more seroepidemiological investigations are needed to estimate the disease burden accurately.…”