Genetic characterization of Curraleiro Pé-Duro bovine breed from a conservation herd of Brazilian semiarid

Filho, Ednaldo da Silva; Silva, M H; Campêlo, José Elivalto Guimarães; Derosia, M R; Pinheiro, Luiz Marcelo de Lima; Almeida, M.J.O.

doi:10.4238/2014.march.24.22

Cited by 4 publications

(1 citation statement)

References 10 publications

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“…The allele and genotype frequencies were high for the wild alleles and genotypes in the majority of SNPs, which explains the high degree of consanguinity, as such the genotype frequencies observed and expected exhibited significant differences (P < 0.05), resulting in deviations from the Hardy-Weinberg equilibrium as observed in the same manner for microsatellite markers in bovines (Silva Filho et al, 2014).…”

Section: Discussionmentioning

confidence: 72%

Polymorphisms in the melatonin receptor gene promoter and their associations with fertility characteristics in buffalo herd in Eastern Amazon

et al. 2017

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ABSTRACT. Buffalo production is spreading globally because of its economic advantage. Then, it has become necessary to improve the reproductive and productive efficiency of these animals, as well as to look for genetic factors that increase this efficiency. The objectives of this study were to characterize the promoter region of the melatonin 1A receptor gene (MTRN1A), to detect possible SNPs and associate them with fertility characteristics, and identify binding sites of transcription factors involved in the regulation of genetic expression 2 E.M. Barbosa et al. Genetics and Molecular Research 16 (2): gmr16029610 in buffaloes in the Amazon. The conventional PCR method was carried out using the two primers designed from the reference sequence deposited in the GenBank AY52466.1. The products of the PCRs were purified, sequenced, and subsequently edited and aligned. Twenty-six SNPs were found, where 73% presented allele frequencies of wild nucleotides above 0.5, and 73% presented deviations from the HardyWeinberg equilibrium (P < 0.05) and F IS varying between 0.06 and 1.00, characterizing high degrees of inbreeding within the population. A block of ACAA deletion (position -1483) was observed in 25% of samples. The associations between these SNPs and reproductive characteristics were observed for calving interval and 5 SNPs: -1289, -1139, -911, -724, and -656 (P < 0.05), and three other SNPs: -1395, -724, and -94 (P < 0.05) were associated significantly with age at first calving, and were not associated with calving concentration. The promoter region was characterized by the different types of binding factors, where only 11 sites are significantly strong enough for transcription factor bindings. The ACAA deletion also exhibited a strong association with transcription factors. As a result, it would be necessary to test the SNPs above with other reproductive characteristics of economic relevance to approve the gene as a strong candidate for the selection of buffaloes in the Amazon.

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Section: Discussionmentioning

confidence: 72%