Genetic Characterization of Corynebacterium pseudotuberculosis Isolates in Egypt

Abstract

Corynebacterium pseudotuberculosis is a small Gram-positive bacillus containing mycolic acid in the structure of the cell wall. The bacterium is responsible for Caseous Lymphadenitis (CLA) in small ruminants (sheep and goats). The bacteria are also responsible for Ulcerative Lymphangitis in equines. The disease causes great economic loss in the animal industry. This work aimed to check the ability of Quadruplex PCR (Q-PCR) for genotyping and identification of Egyptian isolates of C. pseudotuberculosis and sequence analysis of phospholipase D (PLD) gene of local isolates. Four of C. pseudotuberculosis local isolates previously biochemically identified were tested for narG gene (nitrate reductase gene). Both nitrate negative biovar (ovis) and nitrate positive biovar (equi) showed a positive result for 16S rRNA, rpoB and PLD genes of C. pseudotuberculosis species. The sequence analysis of our local isolates' PLD gene revealed minor changes in PLD proteins between ovine and equine strains compared with other published PLD sequences in GenBank. It was concluded that the Q-PCR method is able to differentiate between C. pseudotuberculosis equi and ovis biovars. Also, the sequence of PLD gene of local isolates representing the two biovars revealed some variation, which leads to an accurate diagnosis of C. pseudotuberculosis biovars and generates a mapping of immerged local isolates. Further, the PCR and sequence of these isolates provide rapid and accurate genotyping, especially with hyperimmune serum's unavailability.

Screening of Some Biotic and Abiotic Agents for Controlling Maize Late Wilt Disease Caused by <i>Magnaporthiopsis maydis</i>

“…Autoclaved glass bottles (500 gm in capacity) each contained about 100 gm of wet sorghum grains were used for inoculum preparation. Prepared bottles were inoculated with pieces (2 cm 2 ) of 5-days old M. maydis culture and incubated at room temperature (27-30°C) for about 3-4 weeks [25] for reaching sufficient growth of the fungus. Obtained fungal mass were poured out and mixed with loam soil at the rate of 100g of M. maydis colonized seeds per 10Kg potted soil.…”

Pathogenic and Non-pathogenic Factors; Especially Infectious Bursal Disease Viruses; Affect Chicken Digestive System Microbiota and Methods of Its Evaluation and Recovery: A review

“…It expanded to Australia, the Far East, India, the Middle East, and southern and western Africa in the late 1960s and early 1970s [134][135][136] . In Egypt, the infectious bursal disease virus was originally discovered in 1974 [137] .…”

Epidemiological Occurrence of the Infectious Bursal Disease Virus in Chickens’ Flocks that Had Received Various Vaccination Regimens

“…In Egypt, EL-Sergany et al diagnosed the IBD for the first time based on its specific pathological lesions [15]. IBDV outbreaks continue to infect broiler chickens, resulting in severe economic losses despite mandated vaccination against the disease.…”