Genetic characterization of an Italian Giant Reed (Arundo donax L.) clones collection: exploiting clonal selection

Pilu, Roberto; Cassani, E.; Landoni, M.; Badone, Francesco Cerino; Passera, Alessandro; Cantaluppi, Enrico; Corno, Luca; Adani, Fabrizio

doi:10.1007/s10681-013-1022-z

Cited by 42 publications

(49 citation statements)

References 36 publications

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“…Notably, the wide change of H 2 estimates for Rs july (H 2 = 0.28) and Rs august (H 2 = 0.62) is to be expected because stomatal resistance is an instantaneously measured physiological trait related to leaf gas exchange (Ackerly et al, 2000). Other studies in A. donax have also observed moderate H 2 in biomass and morphology traits (Cosentino et al, 2006;Pilu et al, 2014). Individual traits with higher H 2 (morphology, biomass) tended to be intercorrelated.…”

Section: Discussionmentioning

confidence: 84%

“…Thus, the natural variability in existing populations may occur following spontaneous mutations selected by natural pressure (Cosentino, Copani, D'Agosta, Sanzone, & Mantineo, 2006). Despite the low genetic diversity revealed in a few studies using molecular markers (Ahmad, Liow, Spencer, & Jasieniuk, 2008;Hardion, Verlaque, Baumel, Juin, & Vila, 2012;Hardion, Verlaque, Saltonstall, Leriche, & Vila, 2014;Mariani et al, 2010;Pilu et al, 2014), heritable phenotypic differences have been described in growth-related traits (Cosentino et al, 2006;Pilu et al, 2014). For this reason, ecotype selection remains important for genetic improvement of A. donax (Corno et al, 2014;Pilu et al, 2013).…”

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confidence: 99%

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Characterization of phenology, physiology, morphology and biomass traits across a broad Euro‐Mediterranean ecotypic panel of the lignocellulosic feedstock Arundo donax

Fabbrini

Ludovisi

Alasia³

et al. 2018

GCB Bioenergy

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Giant reed (Arundo donax L.) is a perennial rhizomatous grass, which has attracted great attention as a potential lignocellulosic feedstock for bioethanol production due to high biomass yield in marginal land areas, high polysaccharide content and low inhibitor levels in microbial fermentations. However, little is known about the trait variation that is available across a broad ecotypic panel of A. donax nor the traits that contribute most significantly to yield and growth in drought prone environments. A collection of 82 ecotypes of A. donax sampled across the Mediterranean basin was planted in a common garden experimental field in Savigliano, Italy. We analysed the collection using 367 clumps representing replicate plantings of 82 ecotypes for variation in 21 traits important for biomass accumulation and to identify the particular set of ecotypes with the most promising potential for biomass production. We measured morpho‐physiological, phenological and biomass traits and analysed causal relationships between traits and productivity characteristics assessed at leaf and canopy levels. The results identified differences among the 82 ecotypes for all studied traits: those showing the highest level of variability included stomatal resistance, stem density (StN), stem dry mass (StDM) and total biomass production (TotDM). Multiple regression analysis revealed that leaf area index, StDM, StN, number of nodes per stem, stem height and diameter were the most significant predictors of TotDM and the most important early selection criteria for bioenergy production from A. donax. These traits were used in a hierarchical cluster analysis to identify groups of similar ecotypes, and a selection was made of promising ecotypes for multiyear and multisite testing for biomass production. Heritability estimates were significant for all traits. The potential of this ecotype collection as a resource for studies of germplasm diversity and for the analysis of traits underpinning high productivity of A. donax is highlighted.

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Section: Discussionmentioning

confidence: 84%

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confidence: 99%

Characterization of phenology, physiology, morphology and biomass traits across a broad Euro‐Mediterranean ecotypic panel of the lignocellulosic feedstock Arundo donax

Fabbrini

Ludovisi

Alasia³

et al. 2018

GCB Bioenergy

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“…Moreover, recent studies indicated restricted variability in overall water use and CO 2 gas exchange, and therefore biomass yield, in response to water stress of several giant reed genotypes (Mann et al 2013, Nackley et al 2014, Sanchez et al 2015, Pompeiano et al 2016, Romero-Munar et al 2017, Haworth et al 2017c. Even though such restricted variability could be related to the limited epigenetic variability among giant reed genotypes (Cosentino et al 2006, Ahmad et al 2008, Mariani et al 2010, Pilu et al 2014, Haworth et al 2017b, 2017c, Haworth et al (2017b) found different adaptive hydraulic stem conductivities in giant reed genotypes (the ones used in the present study) from two contrasting environments (Morocco and Northern Italy), due to the species plasticity to adapt to the prevailing climatic conditions and common abiotic stress in the respective regions of origin. Ahrar et al (2017) also showed large DR adaptation plasticity at the photosynthetic level of Bulgarian and Italian genotypes.…”

Section: Introductionmentioning

confidence: 99%

Giant reed genotypes from temperate and arid environments show different response mechanisms to drought

Zegada‐Lizarazu

Rocca

Centritto

et al. 2018

Physiologia Plantarum

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Studies at the root level and how the root-shoot interactions may influence the whole crop performance of giant reed (Arundo donax L.) under limited water conditions are largely missing. In the present study, we illustrate the effects of water stress on some phenotypic traits at the root-shoot levels of two giant reed genotypes (from Morocco and Northern Italy) that were reported to have different adaptive hydraulic stem conductivities despite the limited genetic variability of the species. The trial was carried out in 1 m rhizotrons (1 × 1 × 1 m) for two consecutive growing seasons. As expected, both genotypes showed an effective behavior to contrast water shortage; however, the Moroccan genotype showed a higher leaf water potential, a lower root length density (RLD) and thinner roots in the upper soil layer (0-20 cm), and similar to control RLD values at deep soil layers (40-60 cm). On the other hand the Italian genotype showed the opposite pattern; that is no drought (DR) effects in RLD and root diameter at upper soil layers and reduced RLD in deep layers, thus revealing different DR adaptation characteristics between two genotypes. This DR adaptation variability might bring new insights on DR tolerance of giant reed identifying potential traits aimed to improve the integral plant functioning, to a more efficient use of water resources, and to a more effective crop allocation to targeted stressful conditions under a climate change scenario that foresees the increase of DR periods.

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“…However, despite its low genetic diversity, some phenotypic differences have been reported in the literature . As consequence of that, in 2010, a clone selection programme was established at the University of Milan by collecting 86 clones from Italian territories . The scope of that selection was to identify the best clones for energy and biorefinery purposes.…”

Section: Introductionmentioning

confidence: 99%

Sugars Production for Green Chemistry from 2^nd Generation Crop (Arundo donax L.): A Full Field Approach.

et al. 2016

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Arundo donax L. (giant cane) is a suitable feedstock for sugar production because of its high biomass yield and low agronomic input requirement. Eight A. donax clones were studied at full field scale for sugars production. 1‐ethyl‐3‐methylimidazolium acetate ‐ [C2C1im][OAc] ‐ and enzymatic treatments were used to obtain the sugars. Highest glucose yields were obtained for pretreatment performed at 160 °C for 3 hours, with glucan conversion yields from 40.8 % to 76.2 % for most productive A. donax clones (AD10 and AD 20). Differences in cell wall structure measured by micropore surface area (pores of 0.3 ‐ 1.5 nm) explained both ionic liquids and enzymatic performances of clones. Structural differences were due to the guaiacyl (G) and syringyl (S) units that determined different lignin cross‐linking affecting cell wall microporosity and so enzyme accessibility. Total glucose and xylose yields (11 Mg Ha−1 and 4.84 Mg Ha−1, clone AD20), were impressive and about 3.5 to 4.5 times more than those obtainable from switchgrass and corn stover.

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Genetic characterization of an Italian Giant Reed (Arundo donax L.) clones collection: exploiting clonal selection

Cited by 42 publications

References 36 publications

Characterization of phenology, physiology, morphology and biomass traits across a broad Euro‐Mediterranean ecotypic panel of the lignocellulosic feedstock Arundo donax

Characterization of phenology, physiology, morphology and biomass traits across a broad Euro‐Mediterranean ecotypic panel of the lignocellulosic feedstock Arundo donax

Giant reed genotypes from temperate and arid environments show different response mechanisms to drought

Sugars Production for Green Chemistry from 2^nd Generation Crop (Arundo donax L.): A Full Field Approach.

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Genetic characterization of an Italian Giant Reed (Arundo donax L.) clones collection: exploiting clonal selection

Cited by 42 publications

References 36 publications

Characterization of phenology, physiology, morphology and biomass traits across a broad Euro‐Mediterranean ecotypic panel of the lignocellulosic feedstock Arundo donax

Characterization of phenology, physiology, morphology and biomass traits across a broad Euro‐Mediterranean ecotypic panel of the lignocellulosic feedstock Arundo donax

Giant reed genotypes from temperate and arid environments show different response mechanisms to drought

Sugars Production for Green Chemistry from 2nd Generation Crop (Arundo donax L.): A Full Field Approach.

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Product

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Sugars Production for Green Chemistry from 2^nd Generation Crop (Arundo donax L.): A Full Field Approach.