Genetic Characterization and Pathogenicity of <i>Rhizoctonia solani</i> Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina

Spedaletti, Yamila Andrea; Aparicio, M.; Cárdenas, G.; Rodriguero, Marcela Silvina; Taboada, Gisel María; Aban, Carla Luciana; Sühring, Silvia; Vizgarra, Oscar N.; Galván, Marta Zulema

doi:10.1111/jph.12526

Cited by 11 publications

(12 citation statements)

References 30 publications

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“…We also observed color variability in our study as also observed by Sanfuentes et al (2007). Spedaletti et al (2016) showed that 62.9% of the colonies of Rhizoctonia solani grown in PDA had a light brown coloration and that only 20.6%…”

Section: Discussionsupporting

confidence: 87%

Characterization, pathogenicity and anastomosis groups of Rhizoctonia solani from watermelon

et al. 2019

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The objective of this study was to characterize the morphological and pathogenic variation of Rhizoctonia solani isolates as well as to determine mycelial compatibility and hyphal fusion. The R. solani isolates CMM1053, CMM2967, CMM1052, CMM2983, CMM2971 and CMM3890 from watermelon were used. The determination of aggressiveness was evaluated using the six isolates inoculated in the Crimson Sweet susceptible cultivar in a completely randomized design (CRD) with five replicates, the sample unit consisting of one plant. The experiment of mycelial growth rate was installed in the factorial scheme, 6 isolates x 3 culture media, using the following culture media Nutrient Agar, PDA and PSA, and a total of 5 replicates. The color characterization and sclerotia formation was performed 15 days after the fungal inoculation in each culture medium. For the characterization of vegetative compatibility and occurrence of hyphal fusion, the experiments were performed in CDR with three and two replicates, respectively. CMM1053 and CMM1052 isolates were the most aggressive; however, they were statistically different only from CMM2967 isolate. The PSA medium was the most promising for the mycelial growth. It was possible to observe that there was variability in the colonies color, being higher in the Nutrient Agar medium. Based on evaluations of vegetative compatibility and hyphal fusion, the six isolates belong to the same anastomosis group.

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Section: Discussionsupporting

confidence: 87%

Characterization, pathogenicity and anastomosis groups of Rhizoctonia solani from watermelon

et al. 2019

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“…Valentín Torres et al (2016) demonstrated that isolates from both leaves and roots were able to induce WB symptoms. Spedaletti et al (2016) conducted pathogenicity tests of AG-2-2 isolates collected in northwestern Argentina from common bean expressing WB symptoms. Most of the isolates caused disease symptoms on leaves but a few isolates also aggressively infected hypocotyls.…”

Section: Crop Sciencementioning

confidence: 99%

“…Web blight is endemic in Central America and the Caribbean (Godoy‐Lutz et al., 2008; Rodríguez et al., 1999b; Rajnauth, 1987), the Brazilian Amazon agroecosystem (Alves de Souza et al., 2014; de Jesus Boari et al., 2020; Chavarro‐Mesa et al., 2020), northwestern Argentina (Spedaletti et al., 2016), Ecuador (Villamar‐Torres et al., 2018), and Africa (Masangano & Miles, 2004; Wortmann et al., 1998). Upmanyu and Gupta (2005) reported that WB is a serious disease of snap bean ( P. vulgaris L. var.…”

Section: Introductionmentioning

confidence: 99%

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Breeding for resistance and integrated management of web blight in common bean

Beaver

Figueroa²,

Lutz³

et al. 2021

Crop Science

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Common bean (Phaseolus vulgaris L.) production in the humid tropics may increase if less predictable rainfall becomes a serious seed yield constraint in traditional areas of production. The successful production of common bean will require the effective management of common bean diseases favored by hot and humid conditions. Web blight (WB), caused by Thanatephorus cucumeris (Frank) Donk, is a serious disease of common bean in the humid tropics causing significant losses in both seed yield and seed quality. At present, only moderate levels of WB resistance have been identified. Leaf and seed damage and leaf lesion size are criteria that have been successfully used to screen common bean genotypes for WB resistance in the greenhouse and in the field. Pedigree selection has been successfully used to develop cultivars having moderate levels of resistance. Recurrent selection has been used to broaden the genetic base and to produce progeny having higher levels of WB resistance. Sources of resistance from secondary gene pools should be used to combine resistance genes from diverse sources. Common bean cultivars with earlier maturity, deep tap roots, and terminal drought tolerance may be desirable traits for farmers who plant later in the wet season to avoid WB. Seed production during the dry season using irrigation would help to reduce infection of the seed and transmission of the WB pathogen. Minimum tillage techniques are useful to help to reduce the spread of the disease. Biological control may contribute to the management of WB and complement genetic resistance.

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“…Rhizoctonia solani Kuhn (the teleomorph of T. cucumeris ) is a cosmopolitan pathogen in soils that can destroy a wide host range of plants including rice, corn, potato, tomato, Mentha , bean, soybean, lucerne and lettuce, in both tropical and temperate parts of the world ( Taheri, Gnanamanickam & Höfte, 2007 ; Li, Wu & Yan, 1998 ; Chen et al, 2016 ; Bartz et al, 2010 ; Nitzan et al, 2012 ; Spedaletti et al, 2016 ; Fenille, De Souza & Kuramae, 2002 ; Anderson et al, 2004 ; Grosch, Schneider & Kofoet, 2004 ). Peanut sheath blight disease caused by R. solani has been reported in Liaoning Province, China.…”

Section: Introductionmentioning

confidence: 99%

Cell-wall-degrading enzymes produced in vitro and in vivo byRhizoctonia solani, the causative fungus of peanut sheath blight

Xue¹,

Zhou²,

Li³

et al. 2018

PeerJ

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Rhizoctonia solani causes the disease peanut sheath blight, involving symptoms of maceration and necrosis of infected tissue, mainly caused by cell-wall-degrading enzymes (CWDEs). This study investigated the production of CWDEs including polygalacturonase (PG), polymethyl-galacturonase (PMG), cellulase (Cx) and β-glucosidase by R. solani in vitro (in liquid culture) and in vivo (in peanut plants). Significant PG, PMG, Cx and β-glucosidase activities were detected in infected tissues including stalk and leaves of Baisha and Silihong peanut cultivars. Extracts of healthy tissue showed little or no such activities. In shaken liquid cultures of R. solani in medium containing pectin or pectin plus carboxymethyl cellulose (CMC) as the carbon source(s), PG and PMG were notably active. Significant Cx activity was detected in cultures with CMC or pectin plus CMC as the carbon source(s). However, only a very low level of β-glucosidase activity was observed in cultures with any of the tested carbon sources. An increase of pH was recorded in decayed peanut tissues and liquid culture filtrates; the filtrate pH and fungal growth positively correlated. The fungal growth and/or pH were important factors for the production of PG, PMG and Cx in culture with pectin plus CMC as the carbon source. A single active PG isozyme with isoelectric point around 9.2 was detected in culture filtrates and in infected peanut tissues by the method of isoelectric focusing electrophoresis. The crude enzymes extracted from liquid culture of R. solani induced decay of healthy peanut leaves.

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Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina

Cited by 11 publications

References 30 publications

Characterization, pathogenicity and anastomosis groups of Rhizoctonia solani from watermelon

Characterization, pathogenicity and anastomosis groups of Rhizoctonia solani from watermelon

Breeding for resistance and integrated management of web blight in common bean

Cell-wall-degrading enzymes produced in vitro and in vivo byRhizoctonia solani, the causative fungus of peanut sheath blight

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