Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar

Abstract

Phytophthora root rot (PRR), caused by Phytophthora sojae Kaufmann & Gerdemann, is one of the most destructive diseases of soybean [Glycine max (L.) Merr.]. Deployment of resistance genes is the most economical and effective way of controlling the disease. The soybean cultivar 'Yudou 29' is resistant to many P. sojae isolates in China. The genetic basis of the resistance in 'Yudou 29' was elucidated through an inheritance study and molecular mapping. In response to 25 P. sojae isolates, 'Yudou 29' displayed a new resistance reaction pattern distinct from those of differentials carrying known Rps genes. A population of 214 F2:3 families from a cross between 'Jikedou 2' (PRR susceptible) and 'Yudou 29' was used for Rps gene mapping. The segregation fit a ratio of 1:2:1 for resistance:segregation:susceptibility within this population, indicating that resistance in 'Yudou 29' is controlled by a single dominant gene. This gene was temporarily named RpsYD29 and mapped on soybean chromosome 03 (molecular linkage group N; MLG N) flanked by SSR markers SattWM82-50 and Satt1k4b at a genetic distance of 0.5 and 0.2 cM, respectively. Two nucleotide binding site-leucine rich repeat (NBS-LRR) type genes were detected in the 204.8 kb region between SattWM82-50 and Satt1k4b. These two genes showed high similarity to Rps1k in amino acid sequence and could be candidate genes for PRR resistance. Based on the phenotype reactions and the physical position on soybean chromosome 03, RpsYD29 might be a novel allele at, or a novel gene tightly linked to, the Rps1 locus.

De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits

“…TIRonly and TIR-X sequences were not included in this study. For R-genes with CNV, the expression analysis result and related traits reported in previous studies [25][26][27][28][77][78][79] were listed in Supplementary Table 23. …”

“…The G. soja genome contained a broader range of NBS R-gene domain architectures than did G. max, but G. max had more NBS-encoding genes (460 versus 334 in GsojaA to 382 in GsojaD) (Supplementary Table 22). R-genes showed more copy number loss in G. soja accessions (88 lost versus 33 gained and 8 copy number gain/loss, Supplementary Table 23), several of which were known to confer resistance to soybean mosaic virus 25 , Asian soybean rust 26 and Phytophthora root rot 27 , and be involved in legume-rhizobia symbiosis 28 . In addition, five structurally diverged regions (located on chromosomes 03, 06, 07 and 18) within cultivated G. max 29 , had a high frequency of CNV in the pan-genome (Supplementary Fig.…”

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

“…With the exception of phytophthora root rot for which both monogenic race-specific resistance and quantitative resistance have been described (Lee et al, 2013c;Zhang et al, 2013), resistance to root rot is exclusively quantitative and of complex inheritance (Montoya et al, 1997;Grunwald et al, 2003;Roman-Aviles and Kelly, 2005;Zhao et al, 2005Zhao et al, , 2009Ellis et al, 2013;Hamon et al, 2013). Resistance to Fusarium solani f.sp.…”

Races ofPhytophthora sojaein Ontario, Canada, 2010–2012

“…During this period, it was determined that widespread cultivars 'Harosoy ' (1951) and 'Maple Arrow' contained additional Rps genes, which had not yet been discovered at the time of their release: Rps7 in 'Harosoy' (Anderson & Buzzell 1992b;Dorrance et al 2004) and Rps6 in 'Maple Arrow' (Athow & Laviolette 1982;Anderson et al 2007). New Rps genes or alleles are still being discovered (Burnham et al 2003;Lin et al 2013;Zhang et al 2013;Sun et al 2014), and one of these, Rps8 (Burnham et al 2003), is in the process of being deployed in cultivars in the USA (St. Martin et al 2008).…”