Genetic characterization and fine mapping BrCER4 in involved cuticular wax formation in purple cai-tai (Brassica rapa L. var. purpurea)

Wang, Canjie; Li, Honglian; Li, Yixiao; Qiu-feng, Meng; Xie, Fei; Xu, Yuejin; Wan, Zhengjie

doi:10.1007/s11032-018-0919-6

Cited by 18 publications

(8 citation statements)

References 44 publications

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“…Four of these genes were identified in our selective sweep and differential gene expression analyses. Mapping studies previously identified these genes as being associated with leaf color variation ( Bra006208 ; Fu et al ., 2019), cuticular wax biosynthesis ( Bra011470 and Bra032670 ; Wang et al ., 2017, 2019), and clubroot resistance ( Bra019410 ; Yu et al ., 2016). For other loci, mapping studies have identified a small collection of candidate genes in target regions.…”

Section: Resultsmentioning

confidence: 99%

Genes derived from ancient polyploidy have higher genetic diversity and are associated with domestication in Brassica rapa

Hall

et al. 2021

New Phytologist

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Summary Many crops are polyploid or have a polyploid ancestry. Recent phylogenetic analyses have found that polyploidy often preceded the domestication of crop plants. One explanation for this observation is that increased genetic diversity following polyploidy may have been important during the strong artificial selection that occurs during domestication. In order to test the connection between domestication and polyploidy, we identified and examined candidate genes associated with the domestication of the diverse crop varieties of Brassica rapa. Like all ‘diploid’ flowering plants, B. rapa has a diploidized paleopolyploid genome and experienced many rounds of whole genome duplication (WGD). We analyzed transcriptome data of more than 100 cultivated B. rapa accessions. Using a combination of approaches, we identified > 3000 candidate genes associated with the domestication of four major B. rapa crop varieties. Consistent with our expectation, we found that the candidate genes were significantly enriched with genes derived from the Brassiceae mesohexaploidy. We also observed that paleologs were significantly more diverse than non‐paleologs. Our analyses find evidence for that genetic diversity derived from ancient polyploidy played a key role in the domestication of B. rapa and provide support for its importance in the success of modern agriculture.

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Section: Resultsmentioning

confidence: 99%

Genes derived from ancient polyploidy have higher genetic diversity and are associated with domestication in Brassica rapa

Hall

et al. 2021

New Phytologist

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“…A gene homologous to SHINE3 was identified by conducting a nucleotide analysis. A 39 bp deletion detected in the third exon of the brcer4 gene in the smooth mutant of Porphyra stipa resulted in a premature stop codon in the transcript of the smooth mutant [ 36 ]. In the present study, a 35 bp deletion in the exon region of the BrSHINE3 gene in smooth plants resulted in aberrant mRNA transcription.…”

Section: Discussionmentioning

confidence: 99%

The AP2 Transcription Factor BrSHINE3 Regulates Wax Accumulation in Nonheading Chinese Cabbage

Huo

Yuan

et al. 2022

IJMS

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Wax is an acellular structural substance attached to the surface of plant tissues. It forms a protective barrier on the epidermis of plants and plays an important role in resisting abiotic and biotic stresses. In this paper, nonheading Chinese cabbage varieties with and without wax powder were observed using scanning electron microscopy, and the surface of waxy plants was covered with a layer of densely arranged waxy crystals, thus differentiating them from the surface of waxless plants. A genetic analysis showed that wax powder formation in nonheading Chinese cabbage was controlled by a pair of dominant genes. A preliminary bulked segregant analysis sequencing (BSA-seq) assay showed that one gene was located at the end of chromosome A09. Within this interval, we identified BraA09000626, encoding an AP2 transcription factor homologous to Arabidopsis AtSHINE3, and we named it BrSHINE3. By comparing the CDS of the gene in the two parental plants, a 35 bp deletion in the BrSHINE3 gene of waxless plants resulted in a frameshift mutation. Tissue analysis showed that BrSHINE3 was expressed at significantly higher levels in waxy plant rosette stage petioles and bolting stage stems than in the tissues of waxless plants. We speculate that this deletion in BrSHINE3 bases in the waxless material may inhibit wax synthesis. The overexpression of BrSHINE3 in Arabidopsis induced the accumulation of wax on the stem surface, indicating that BrSHINE3 is a key gene that regulates the formation of wax powder in nonheading Chinese cabbage. The analysis of the subcellular localization showed that BrSHINE3 is mainly located in the nucleus and chloroplast of tobacco leaves, suggesting that the gene may function as a transcription factor. Subsequent transcriptome analysis of the homology of BrSHINE3 downstream genes in nonheading Chinese cabbage showed that these genes were downregulated in waxless materials. These findings provide a basis for a better understanding of the nonheading Chinese cabbage epidermal wax synthesis pathway and provide important information for the molecular-assisted breeding of nonheading Chinese cabbage.

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“…Deletion of BrWAX2 and decreased expression of other genes in the alkane formation pathway reduced wax content and led to a glossy phenotype (Yang et al., 2022). The ECERIFERUM4 ( BrCER4 ) was mapped from different glossiness purple cai‐tai lines, which was involved in the regulation of cuticular wax formation (Wang et al., 2019). The expression of BrCER4 gene was downregulated in the glossy cai‐tai mutant, and the epidermal ultrastructure of the leaf and stem showed that the wax content of the mutant was lower than that of the wild type, indicating that BrCER4 negatively participated in the glossy phenotype of cai‐tai through wax synthesis (Wang et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

The COPII subunit CsSEC23 mediates fruit glossiness in cucumber

et al. 2023

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SUMMARYTo improve our understanding of the mechanism underlying cucumber glossiness regulation, a novel cucumber mutant with a glossy peel (Csgp) was identified. MutMap, genotyping, and gene editing results demonstrated that CsSEC23, which is the core component of COPII vesicles, mediates the glossiness of cucumber fruit peel. CsSEC23 is functionally conserved and located in the Golgi and endoplasmic reticulum. CsSEC23 could interact with CsSEC31, but this interaction was absent in the Csgp mutant, which decreased the efficiency of COPII vesicle transportation. Genes related to wax and cutin transport were upregulated in the Csgp mutant, and the cuticle structure of the Csgp‐mutant peel became thinner. Moreover, the wax and cutin contents were also changed due to CsSEC23 mutation. Taken together, the results obtained from this study revealed that CsSEC23 mediates cucumber glossiness, and this mediating might be affected by COPII vesicle transportation.

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Genetic characterization and fine mapping BrCER4 in involved cuticular wax formation in purple cai-tai (Brassica rapa L. var. purpurea)

Cited by 18 publications

References 44 publications

Genes derived from ancient polyploidy have higher genetic diversity and are associated with domestication in Brassica rapa

Genes derived from ancient polyploidy have higher genetic diversity and are associated with domestication in Brassica rapa

The AP2 Transcription Factor BrSHINE3 Regulates Wax Accumulation in Nonheading Chinese Cabbage

The COPII subunit CsSEC23 mediates fruit glossiness in cucumber

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