Genetic characterization and DNA barcoding of coffee shot-hole borer,
            <i>Xylosandrus compactus</i>
            (Eichhoff) (Coleoptera: Curculionidae: Scolytinae)

Kiran, V. S.; Asokan, R.; Revannavar, Revanna; Mallesh, Mahadeva Swamy Hanchipura; Ramasamy, Ellango

doi:10.1080/24701394.2019.1659249

Cited by 5 publications

(5 citation statements)

References 13 publications

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“…Therefore, the possibility of using a molecular method to univocally identify the species could be a functional tool in the operative practice, especially in areas where the insect has not been yet detected or in the niches where other Xylosandrus species can overlap their distribution (Francardi et al 2017 ; Contarini et al 2020 ). Molecular identification of X. compactus (Kiran et al 2019 ), X. crassiusculus (Landi et al 2017 ) and X. germanus (Cognato et al 2020 ) has been established based on the mitochondrial cytochrome c oxidase I.…”

Section: Introductionmentioning

confidence: 99%

TaqMan probe assays on different biological samples for the identification of three ambrosia beetle species, Xylosandrus compactus (Eichoff), X. crassiusculus (Motschulsky) and X. germanus (Blandford) (Coleoptera Curculionidae Scolytinae)

Rizzo¹,

Lio

Bartolini³

et al. 2021

3 Biotech

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Molecular assays based on qPCR TaqMan Probes were developed to identify three species of the genus Xylosandrus, X. compactus, X. crassiusculus and X. germanus (Coleoptera Curculionidae Scolytinae). These ambrosia beetles are xylophagous species alien to Europe, causing damages to many ornamental and fruiting trees as well as shrubs. DNA extraction was carried out from adults, larvae and biological samples derived from insect damages on infested plants. For X. compactus, segments of galleries in thin infested twigs were cut and processed; in the case of X. crassiusculus, raw frass extruded from exit holes was used, while DNA of X. germanus was extracted from small wood chips removed around insect exit holes. The assays were inclusive for the target species and exclusive for all the non-target species tested. The LoD was 3.2 pg/µL for the frass of X. crassiusculus and 0.016 ng/µL for the woody matrices of the other two species. Both repeatability and reproducibility were estimated on adults and woody samples, showing very low values ranging between 0.00 and 4.11. Thus, the proposed diagnostic assays resulted to be very efficient also on the woody matrices used for DNA extraction, demonstrating the applicability of the protocol in the absence of dead specimens or living stages.

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Section: Introductionmentioning

confidence: 99%

TaqMan probe assays on different biological samples for the identification of three ambrosia beetle species, Xylosandrus compactus (Eichoff), X. crassiusculus (Motschulsky) and X. germanus (Blandford) (Coleoptera Curculionidae Scolytinae)

Rizzo¹,

Lio

Bartolini³

et al. 2021

3 Biotech

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“…Ghana and Uganda were invaded by the same mitochondrial haplotype belonging to the lineage B, presumably more than a century ago (Egonyu et al 2015). The haplotype B2, which occurs in Africa, was observed within the native range in a locality in Vietnam and a locality in India, where Kiran et al (2019) sequenced around 200 specimens and found the haplotype B2 exclusively. These two localities are more than 3500 km apart, and a more thorough sampling throughout Asia might have revealed the haplotype B2 in other localities, potentially along the Bay of Bengal.…”

Section: Invasion History Of X Compactus and Plausible Scenariosmentioning

confidence: 99%

“…Very little is known about Xylosandrus compactus genetic structure and invasion history. The only genetic study focusing on X. compactus so far was centred on India and based on a single mitochondrial marker, showing a very low genetic diversity in spite of a sampling including almost 200 specimens (Kiran et al 2019). However, this work did not discuss X. compactus invasion history.…”

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confidence: 99%

A first inference of the phylogeography of the worldwide invader Xylosandrus compactus

et al. 2021

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Native to Southeastern Asia, the ambrosia beetle Xylosandrus compactus is invasive worldwide. Its invasion is favoured by its cryptic lifestyle, symbiosis with a fungus that facilitates a broad range of host plants, and predominant sib-mating reproduction. X. compactus invaded Africa more than a century ago and the Americas and Pacific Islands in the middle of the twentieth century. It was not detected in Europe before 2011, when it was first reported in Italy before quickly spreading to France, Greece and Spain. Despite the negative environmental, agricultural and economic consequences of the invasion of X. compactus, its invasion history and main pathways remain poorly documented. We used COI and RAD sequencing to (i) characterise the worldwide genetic structure of the species, (ii) disentangle the origin(s) of the non-native populations on the three invaded continents and (iii) analyse the genetic diversity and pathways within each invaded region. Three mitochondrial lineages were identified in the native range. Populations invading Europe and the American-Pacific region originated from the first lineage and were only slightly genetically differentiated at nuclear SNP markers, suggesting independent introductions from close sources in or near Shanghai, ca. 60 years apart. Populations invading Africa originated from the second lineage, likely from India or Vietnam.

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“…Researchers have used molecular tools to identify X. compactus . Kiran et al (2019) used the mitochondrial cytochrome c oxidize I (COI) gene as a molecular tool to identify X. compactus . Phylogenetic relationship of the collected population was summarized, which solved the problem of obscure traditional identification.…”

Section: Introductionmentioning

confidence: 99%

An accurate, efficient, and economical identification technology for black twig borer based on species-specific cytochrome C oxidase subunit I PCR assay

Sun

et al. 2023

Journal of Economic Entomology

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Xylosandrus compactus (Eichhoff) (Coleoptera: Curculionidae, Scolytinae) is a worldwide invasive species that causes huge economic loss and environmental damage in many countries. Traditional morphological characteristics make it hard to identify scolytines due to their tiny size. Besides, the intercepted insect samples are incomplete, and the limitation of insect (larvae and pupae) morphology makes morphological identification more difficult. The majority of the damage is caused by adults and fungi that serve as nutrition for their larvae. They destroy plant trunks, branches, and twigs, affecting plant transport tissues in both weak and healthy plants. An accurate, efficient, and economical molecular identification technique for X. compactus not restricted by professional taxonomic knowledge is necessary. In the present study, a molecular identification tool based on the mitochondrial DNA gene, cytochrome C oxidase subunit I (COI) was developed. A species-specific COI (SS-COI) PCR assay was designed to identify X. compactus regardless of the developmental stage. Twelve scolytines commonly found in eastern China, namely Xylosandrus compactus, X. crassiusculus, X. discolor, X. germanus, X. borealis, X. amputates, X. eupatorii, X. mancus, Xyleborinus saxesenii, Euwallacea interjectus, E. fornicatus, and Acanthotomicus suncei, were included in the study. Additionally, specimens of X. compactus from 17 different areas in China, as well as a specimen collected from the United Stated, were also analyzed. Results demonstrated the accuracy and high efficiency of the assay, regardless of the developmental stage or the type of specimen. These features provide a good application prospect for fundamental departments and can be used to prevent the harmful consequences of the spread of X. compactus.

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Genetic characterization and DNA barcoding of coffee shot-hole borer, Xylosandrus compactus (Eichhoff) (Coleoptera: Curculionidae: Scolytinae)

Cited by 5 publications

References 13 publications

TaqMan probe assays on different biological samples for the identification of three ambrosia beetle species, Xylosandrus compactus (Eichoff), X. crassiusculus (Motschulsky) and X. germanus (Blandford) (Coleoptera Curculionidae Scolytinae)

TaqMan probe assays on different biological samples for the identification of three ambrosia beetle species, Xylosandrus compactus (Eichoff), X. crassiusculus (Motschulsky) and X. germanus (Blandford) (Coleoptera Curculionidae Scolytinae)

A first inference of the phylogeography of the worldwide invader Xylosandrus compactus

An accurate, efficient, and economical identification technology for black twig borer based on species-specific cytochrome C oxidase subunit I PCR assay

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