Genetic characterization and antimicrobial resistance ofStaphylococcus aureusisolated from bovine milk in Tunisia

Said, Myriam Ben; Abbassi, Mohamed Salah; Bianchini, Valentina; Sghaier, Senda; Cremonesi, Paola; Romanò, Alicia; Gualdi, V.; Hassen, Abdennaceur; Luini, M.

doi:10.1111/lam.12672

Cited by 33 publications

(31 citation statements)

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“…Of the 42 penicillin‐resistant isolates, 39 harboured the blaZ gene as previously reported (Ben Said et al . ). Five out of the six tetracycline‐resistant isolates harboured the tet(K) gene and the fusC gene was detected in three out of four fusidic acid‐resistant isolates.…”

Section: Resultsmentioning

confidence: 97%

“…Similarly, high rates of S. aureus in bovine milk have been reported in Tunisia (Ben Said et al . ), but a low percentage was reported in milk samples from clinical bovine mastitis (5%) in the same country (Klibi et al . ).…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, high resistance rates (>50%) to penicillin but <10% to other antimicrobials have been previously reported in Tunisia (Ben Said et al . ; Khemiri et al . ), Korea (Nam et al .…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have reported the transmission of antibiotic-resistant S. aureus, especially methicillin-resistant S. aureus (MRSA) among animals, farmers and environments (Ben Said et al 2016). In addition, MRSA isolates are mainly characterized by a multidrug resistance phenotype (Monaco et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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High occurrence of enterotoxigenic isolates and low antibiotic resistance rates of Staphylococcus aureus isolated from raw milk from cows and ewes

Khémiri

Abbassi

Elghaieb

et al. 2019

Lett Appl Microbiol

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This study aimed to analyse the frequency of genes encoding virulence factors and to characterize resistance profiles of Staphylococcus aureus isolated from raw milk. In total, 47 and 9 S. aureus isolates were recovered from 150 and 100 raw bovine and ovine milk samples, respectively, in Tunisia. The majority of isolates was resistant to penicillin, and no methicillin‐resistant S. aureus was detected. Eighteen and two isolates harboured etd and eta genes respectively. Sixteen enterotoxin‐encoding genes were detected (n, %): sed (25, 44·6%), sec (16, 28·6%), sei (16, 28·6%), seh (13, 23·2%), seln (13, 23·2%), sell (10, 17·8%), seg (9, 16%), selu (8, 14·3%), selq (7, 12·5%), selo (7, 12·5%), selm (7, 12·5%), seb (7, 12·5%), sea (6, 10·7%), selk (3, 5·4%), ser (1, 1·8%) and selp (1, 1·8%). Ten isolates carried the tsst1 gene. All isolates carried the haemolysin toxin (hla, hld and hlg). The immune evasion cluster system‐type B was predominant (20 isolates) followed by C (3 isolates), A and E (1 isolate each). The occurrence of enterotoxigenic S. aureus in raw milk constitutes a potential risk for human health. Significance and Impact of the Study This paper describes the characteristics of Staphylococcus aureus isolated from raw milk samples from healthy cows and ewes collected from small family farms in Tunisia. Fifty‐six strains were analysed by determining their antibiotic susceptibility and genes encoding antibiotic resistance and virulence factors. Methicillin‐resistant strains were not detected, and overall low level of antimicrobial resistance was reported. However, our strains harboured several genes encoding virulence factors and 87·5% of them carried at least one gene encoding for enterotoxins showing a high risk of spread of food‐borne diseases.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

“…Similarly, high resistance rates (>50%) to penicillin but <10% to other antimicrobials have been previously reported in Tunisia (Ben Said et al . ; Khemiri et al . ), Korea (Nam et al .…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High occurrence of enterotoxigenic isolates and low antibiotic resistance rates of Staphylococcus aureus isolated from raw milk from cows and ewes

Khémiri

Abbassi

Elghaieb

et al. 2019

Lett Appl Microbiol

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“…Varia com a distribuição geográfica, mas alta incidência de resistência a penicilinas naturais ou semissintéticas, frequentemente utilizadas no tratamento da mastite, está sempre presente (OLIVER;MURINDA, 2012;RUEGG et al, 2015;THOMAS et al, 2015;WANG et al, 2015;BEN SAID et al, 2016;MEHMETI et al, 2016). (KOWALSKI et al, 2015) e no caso dos EUA 9 anos (LINDEMAN et al, 2013).…”

Section: Perfil De Resistência Aos Demais Antimicrobianosunclassified

Estudo da diversidade molecular de linhagens de Staphylococcus aureus de isolados de mastite bovina

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sendo os três mais frequentes t605 (57,45%), t002 (8,4%) e t127 (8,13%). Não houve associação entre a distribuição dos spa tipos e as variáveis analisadas de ano, estado, município e fazenda, e perfil de resitência aos antibicrobianos. Este é o primeiro estudo que utiliza o spa typing para avaliar uma coleção temporalmente diversa e, portanto, representativa da região estudada ao longo de 22 anos. Logo, espera-se que a descrição dos tipos de S. aureus obtidos contribua para a compreensão dos aspectos epidemiológicos envolvidos na transmissão do patógeno e do papel dos hospedeiros como reservatórios para linhagens resistentes. Tal compreensão é imprescindível para a prevenção, controle e tratamento da mastite bovina.Palavras Chaves: Staphylococcus aureus, mastite, antibiograma, mecA, spa Typing. Bovine mastitis is considered the disease of greatest impact in global dairy industry. Despite the adoption of management practices to minimize its occurrence, the results are not satisfactory for the control of Staphylococcus aureus, the major causative agent. Besides veterinary importance, there are also implications for human health due to the extensive use of antibiotics in treatment and control of the disease and due to the potential for zoonotic spread of S. aureus.The main concern regarding antimicrobial resistance is methicillin resistance mediated by the mecA gene. Molecular epidemiology studies are important for the understanding of the genetic diversity of the causative agents of mastitis. Typing by sequencing of a single locus of the X region gene of the S. aureus protein A (spa Typing) has been successfully used to investigate population structure. This project aims to investigate the antimicrobial susceptibility profile and the presence of methicillin resistance genes in S. aureus isolated from bovine mastitis, in addition to understanding the genetic diversity, identifying possible new profiles and knowing the distribution of these isolates. The results obtained to the present were the absence of methicillin resistance in the antibiogram, confirmed by the absence of mecA gene, and the low prevalence of multidrug resistance to antimicrobials. Even though resistance to at least one antibiotic was present in more than 58,74% of the isolates (242/412), ampicillin resistance corresponds to 53.64% (221/412). Different spa types were determined, the three most frequent being t605 (57.45%), t002 (8.4%) and t127 (8.13%). There was no association between the spa type distribution and the variables of year, state, municipality, farm, and antibiotic resistance profile analyzed. This is the first study that uses spa Typing to evaluate a collection as temporarily diverse and representative of the region studied in 22 years. Therefore, it is expected that the results of this study, by allowing comparison of the profile of S. aureus from different sources (animals and property), will contribute to the understanding of the epidemiological aspects involved in the transmission of the pathogen and the role of hosts as reservoi...

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Molecular Epidemiology of Staphylococcus aureus Lineages in the Animal–Human Interface

Zarazaga

Gómez

Ceballos

et al. 2018

Staphylococcus Aureus

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Genetic characterization and antimicrobial resistance ofStaphylococcus aureusisolated from bovine milk in Tunisia

Cited by 33 publications

References 45 publications

High occurrence of enterotoxigenic isolates and low antibiotic resistance rates of Staphylococcus aureus isolated from raw milk from cows and ewes

High occurrence of enterotoxigenic isolates and low antibiotic resistance rates of Staphylococcus aureus isolated from raw milk from cows and ewes

<b>Estudo da diversidade molecular de linhagens de <i>Staphylococcus aureus</i> de isolados de mastite bovina</b>

Molecular Epidemiology of Staphylococcus aureus Lineages in the Animal–Human Interface

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