Genetic characterisation of viruses infecting sweetpotato in South Africa

Mulabisana, J.; Cloete, M.; Mabasa, K.G.; Laurie, S. M.; Oelofse, D.; Rey, M. E. C.

doi:10.17660/actahortic.2015.1105.22

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…The qRT-PCR was performed using a Roche LightCycler 480II system under the following conditions: 95 • C for 15 s, followed by 40 cycles of 95 • C for 15 s, 55 • C for 15 s, and 72 • C for 15 s. The Ib-Actin gene was used as the internal standard, and the data were analyzed using the 2 −△△CT method (Livak & Schmittgen, 2001). Infection of the SPFMV, SPCSV, SPVG, SPLV, and SPV2 virus was detected using nitrocellulose membrane enzymelinked immunosorbent assay (NCM-ELISA) according to the manufacturer's protocol (Mulabisana et al, 2015;Tairo, Kullaya, & Valkonen, 2004). All detections were replicated three times in independent experiments.…”

Section: Dna and Rna Extraction And Quantitative Reverse Transcriptase Polymerase Chain Reaction Assaysmentioning

confidence: 99%

Sweetpotato leaf curl virus decreased storage root yield and quality of edible sweetpotato in China

et al. 2020

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Sweetpotato leaf curl virus (SPLCV) causes leaf curl symptoms in sweetpotato [Ipomoea batatas (L.) Lam]. These symptoms disappear under high temperature. Previous studies have shown that SPCLV reduces storage root yield in sweetpotato. However, knowledge of SPLCV's effect on root quality is limited. In this study, we surveyed the effect of SPLCV on storage root yield and quality in edible sweetpotato. Quantitative real-time reverse transcriptase-polymerase chain reaction and leaf paraffin section results showed that the SPLCV mRNA level and the distribution density of spongy mesophyll cells in SPLCV-infected plants were significantly higher than in healthy control plants (CK). Leaf net photosynthetic rate, vine length, and storage root yield were reduced in SPLCV-infected plants during the growing period, and the shoot/root ratio was significantly increased compared with CK. Additionally, root abscisic acid, auxin, and zeatin riboside contents under SPLCV infection were higher than the CK, whereas root gibberellic acid content was lower. Root dry matter, starch, and carotenoid contents were lower in the infected plants than in the CK. Moreover, SPLCV-infected plants showed reduced expression of starch and carotenoid metabolitic genes, such as ADP-glucose pyrophosphorylase-ß, granule-bound starch synthase I, starch branching enzyme I and II, and carotenoid isomerase, and increased expression of α-amylase, ß-carotene hydroxylase, and zeaxanthin epoxidase. These results suggest that sweetpotato infected by SPLCV had decreased storage root yield and decreased starch and carotenoid contents through reducing photosynthetic capacity, regulating starch and carotenoid degradation, and changing endogenous hormone contents. Therefore, effective management should be taken to prevent SPLCV epidemics during sweetpotato production.

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Section: Dna and Rna Extraction And Quantitative Reverse Transcriptase Polymerase Chain Reaction Assaysmentioning

confidence: 99%

Sweetpotato leaf curl virus decreased storage root yield and quality of edible sweetpotato in China

et al. 2020

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Surveys in the Gauteng, Limpopo and Mpumalanga provinces of South Africa reveal novel isolates of sweet potato viruses

Mulabisana

Cloete

Mabasa

et al. 2018

South African Journal of Botany

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The hunt for plant viruses in South Africa: The early days

Rey

2020

S. Afr. J. Sci.

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Plant viruses cause widespread disease in agriculturally important crops, resulting in a reduction in both quality and quantity of produce. The introduction of intensive crop monoculture has resulted in an exponential increase in viral diseases which can cross over from wild indigenous plants. Viral pathogens also can occur in mixed infections, and rapid, sensitive and reliable diagnostic methods are required to identify and characterise the viruses responsible for the field diseases. In comparison to bacterial and fungal diseases, viral diseases are more difficult to diagnose. This review covers a period (1985–2011) in the history of virus discovery in South Africa during which several plant viruses from commercial and smallscale farms were identified and characterised. Interestingly, novel viruses were discovered in three crops, namely guar and cassava grown by small-scale farmers in Mpumalanga, and in commercial tobacco. The implication of these plant diseases is potential yield loss to farmers which can affect their livelihoods, and result in severe economic loss for the food and agriculture industries. Accurate identification of the causal viral agents of these viral diseases is a prerequisite for development of effective management strategies.

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Genetic characterisation of viruses infecting sweetpotato in South Africa

Cited by 3 publications

References 17 publications

Sweetpotato leaf curl virus decreased storage root yield and quality of edible sweetpotato in China

Sweetpotato leaf curl virus decreased storage root yield and quality of edible sweetpotato in China

Surveys in the Gauteng, Limpopo and Mpumalanga provinces of South Africa reveal novel isolates of sweet potato viruses

The hunt for plant viruses in South Africa: The early days

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