Genetic characterisation of avocado (Persea americana Mill.) in two regions of Ghana

Abraham, Janice Dwomoh; Takrama, Jemmy

doi:10.5897/ajb2014.14023

Cited by 4 publications

(4 citation statements)

References 21 publications

(26 reference statements)

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“…The average observed heterozygosity for the 10 SSR loci obtained in the present study was 0.65 (Table 1) which is similar to 0.64 and 0.61 obtained by Schnell et al [30] and Guzmán et al [31], respectively, indicating similar levels of genetic diversity for their analysed samples. Lower observed heterozygosity have been reported by Abraham and Takrama [32]: 0.48, Boza et al [37]: 0.56, and Liu et al [33]: 0.39, thereby pointing to a lower genetic diversity in the germplasm used or differences in the polymorphism levels of the SSR loci used by them vis-à-vis ours.…”

Section: Discussionmentioning

confidence: 45%

“…In the present study, a total of 167 alleles were detected using 10 SSR loci across 226 sampled avocado trees with the number of alleles ranging from 10 to 23 per locus ( German and Viruel [25] and Abraham and Takrama [32] reported lower numbers, 11.4 and 11.5, respectively. Similarly, Liu et al [33] reported the lowest number of alleles per locus, 3.10, across 10 SSR loci for 56 avocado trees investigated in Hainan Province, China.…”

Section: Discussionmentioning

confidence: 66%

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Genetic diversity of avocado from the southern highlands of Tanzania as revealed by microsatellite markers

et al. 2020

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Background Avocado is an important cash crop in Tanzania, however its genetic diversity is not thoroughly investigated. This study was undertaken to explore the genetic diversity of avocado in the southern highlands using microsatellite markers. A total of 226 local avocado trees originating from seeds were sampled in eight districts of the Mbeya, Njombe and Songwe regions. Each district was considered as a population. The diversity at 10 microsatellite loci was investigated. Results A total of 167 alleles were detected across the 10 loci with an average of 16.7 ± 1.3 alleles per locus. The average expected and observed heterozygosity were 0.84 ± 0.02 and 0.65 ± 0.04, respectively. All but two loci showed a significant deviation from the Hardy-Weinberg principle. Analysis of molecular variance showed that about 6% of the variation was partitioned among the eight geographic populations. Population FST pairwise comparisons revealed lack of genetic differentiation for the seven of 28 population pairs tested. The principal components analysis (PCA) and hierarchical cluster analysis showed a mixing of avocado trees from different districts. The model-based STRUCTURE subdivided the trees samples into four major genetic clusters. Conclusion High diversity detected in the analysed avocado germplasm implies that this germplasm is a potentially valuable source of variable alleles that might be harnessed for genetic improvement of this crop in Tanzania. The mixing of avocado trees from different districts observed in the PCA and dendrogram points to strong gene flow among the avocado populations, which led to population admixture revealed in the STRUCTURE analysis. However, there is still significant differentiation among the tree populations from different districts that can be utilized in the avocado breeding program.

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Section: Discussionmentioning

confidence: 45%

Section: Discussionmentioning

confidence: 66%

Genetic diversity of avocado from the southern highlands of Tanzania as revealed by microsatellite markers

et al. 2020

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“…The 109 avocado trees for this study with twelve molecular markers (SSRs) revealed 140 alleles, with 6 to 16 alleles per locus. Comparable studies include the detection of 11.5 alleles using 10 SSR loci across 71 avocado plants, with alleles ranging from 5 to 22 per locus [27] and the detection of 167 alleles using 10 SSR loci across 226 plants, with the number of alleles per locus ranging from 10 to 23 [5]. In the current analysis, there were 11.67 alleles on average per locus.…”

Section: Microsatellite Polymorphism and Diversitymentioning

confidence: 91%

“…Twelve pairs of microsatellite primers [26]; [27] (Table 1) were used to analyze the collected avocado samples. Forward primers were uorescently labeled for detection in four colors: 6-FAM (Generi Biotech, Czech Republic), PET, NED, and VIC (Thermo Fisher Scienti c, USA).…”

Section: Dna Extraction and Quality Assessment And Ssr Markers And Pc...mentioning

confidence: 99%

Genetic Diversity of Avocado (Persea americana Mill) From Southern Ethiopia Using SSR Markers

Degu

Tadesse

Kalousová

2022

Preprint

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Background: Avocados are a cash crop in Ethiopia. However, its genetic diversity has not been investigated. This study explored the genetic diversity of avocados in southern Ethiopia using SSR markers. Method and Result: One hundred nine avocado trees were sampled in sixteen districts. One hundred forty alleles were detected across the 12 loci. The average number of alleles per locus was 11.7. The average expected and observed heterozygosity was 0.63 ± 0.12 and 0.48 ±0.19, respectively. All loci showed a significant deviation from HWE. The analysis of molecular variance showed that about 5% of the variation was among the sixteen populations. Pairwise comparisons of population FST values revealed a lack of genetic differentiation for seven out of 48 paired comparisons. UPGMA based on Nie and Li similarity index, classified the genotypes into three major classes with 100 and 53 bootstrap values. Genetic distance analyses showed a mixing of avocado trees from different districts. DAPC divided the tree samples into three groups, while the model-based STRUCTURE subdivided the tree samples into two major genetic clusters. Conclusion: Moderate diversity was detected in the analyzed avocado germplasm. These imply, the germplasms are a valuable source of alleles for genetic improvement in Ethiopia. The mixing of avocado trees from different districts observed in UPGMA tree, DAPC, and STRUCTURE analysis. This indicated strong gene flow among the avocado populations. There is, however, significant variation among the tree populations from different districts to be used in the avocado breeding program.

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Exploring genetic variation, oil and α-tocopherol content in avocado (Persea americana) from northwestern Mexico

Peraza-Magallanes

Pereyra-Camacho

Sandoval‐Castro

et al. 2017

Genet Resour Crop Evol

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Genetic characterisation of avocado (Persea americana Mill.) in two regions of Ghana

Cited by 4 publications

References 21 publications

Genetic diversity of avocado from the southern highlands of Tanzania as revealed by microsatellite markers

Genetic diversity of avocado from the southern highlands of Tanzania as revealed by microsatellite markers

Genetic Diversity of Avocado (Persea americana Mill) From Southern Ethiopia Using SSR Markers

Exploring genetic variation, oil and α-tocopherol content in avocado (Persea americana) from northwestern Mexico

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