Genetic characterisation of antibiotic resistance and virulence factors in vanA-containing enterococci from cattle, sheep and pigs subsequent to the discontinuation of the use of avoparcin

Ramos, Sónia; Igrejas, Gilberto; Rodrigues, Jorge; Capelo-Martínez, José Luis; Poeta, Patrícia

doi:10.1016/j.tvjl.2011.12.007

Cited by 26 publications

(22 citation statements)

References 9 publications

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“…One of the main concerns about enterococci is the presence of virulence factors that may contribute to the severity of infections caused by these bacteria. The virulence genes detected in the present work (agg, hyl, and efaAfm) have previously been detected in food-producing animals and in foodstuffs derived from them (4,30,37).…”

Section: Discussionsupporting

confidence: 60%

Capacity of Listeria monocytogenes Strains from the 2011 Cantaloupe Outbreak To Adhere, Survive, and Grow on Cantaloupe

Martinez

Osborne

Jayeola

et al. 2016

Journal of Food Protection

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The 2011 listeriosis outbreak attributed to whole cantaloupe involved several genetically distinct strains of serotypes 1/2a and 1/2b that had not been previously reported in invasive listeriosis outbreaks. Here we investigated the potential of strains from the 2011 cantaloupe outbreak to adhere, survive, and grow on cantaloupe rind and flesh and in juice extracted from cantaloupe at different temperatures (4, 8, and 25°C). All strains were able to adhere and grow, with ∼10-fold increases after 7 days at 4 or 8°C and after 24 h at 25°C, with a propensity for more growth on rind than on flesh or in extract. No significant differences in growth potential were noted among the different strains or between them and unrelated strains from other listeriosis outbreaks involving celery, deli meats, or hot dogs. Similarly to the cantaloupe outbreak strains, these other strains exhibited greater propensity for growth on rind than on flesh or in extract. Rinsing of cantaloupe fragments in sterile water resulted in temporary reductions of the populations by 50- to 100-fold, suggesting the potential of such washing to reduce risk if the produce is promptly consumed. The absence of marked differences in adherence or growth between the cantaloupe outbreak strains and strains from other outbreaks highlights the need to further characterize the 2011 cantaloupe outbreak strains and elucidate potential biological attributes that contributed to their implication in the outbreak.

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Section: Discussionsupporting

confidence: 60%

Capacity of Listeria monocytogenes Strains from the 2011 Cantaloupe Outbreak To Adhere, Survive, and Grow on Cantaloupe

Martinez

Osborne

Jayeola

et al. 2016

Journal of Food Protection

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“…Likewise, Enterococcus are commensal bacteria from the intestinal flora of humans and animals, and are frequently monitored as indicators of fecal contamination of food products (Teuber and Perreten, 2000; Ramos et al, 2012). Enterococci are facultative anaerobes capable of persisting in extreme conditions caused by temperature and pH variations, dehydration, and oxidative stress.…”

Section: Amr Surveillance In Indicator Bacteriamentioning

confidence: 99%

“…Use of avoparcin, a glycopeptide antibiotic chemically similar to vancomycin, as an animal feed additive in Europe, and of high amounts of vancomycin in USA hospitals has led to the spread of VRE in animals, humans, food, and the environment (Kirst et al, 1998; Wegener, 1998). However, banning avoparcin in 2006 has not been sufficient to eradicate the AMR mechanisms present in bacteria and some VRE still persist in animals and the environment (Poeta et al, 2007b; Araujo et al, 2010; Ramos et al, 2012). …”

Section: Amr Surveillance In Indicator Bacteriamentioning

confidence: 99%

A Decade-Long Commitment to Antimicrobial Resistance Surveillance in Portugal

et al. 2016

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Antimicrobial resistance (AMR) is a worldwide problem with serious health and economic repercussions. Since the 1940s, underuse, overuse, and misuse of antibiotics have had a significant environmental downside. Large amounts of antibiotics not fully metabolized after use in human and veterinary medicine, and other applications, are annually released into the environment. The result has been the development and dissemination of antibiotic-resistant bacteria due to many years of selective pressure. Surveillance of AMR provides important information that helps in monitoring and understanding how resistance mechanisms develop and disseminate within different environments. Surveillance data is needed to inform clinical therapy decisions, to guide policy proposals, and to assess the impact of action plans to fight AMR. The Functional Genomics and Proteomics Unit, based at the University of Trás-os-Montes and Alto Douro in Vila Real, Portugal, has recently completed 10 years of research surveying AMR in bacteria, mainly commensal indicator bacteria such as enterococci and Escherichia coli from the microbiota of different animals. Samples from more than 75 different sources have been accessed, from humans to food-producing animals, pets, and wild animals. The typical microbiological workflow involved phenotypic studies followed by molecular approaches. Throughout the decade, 4,017 samples were collected and over 5,000 bacterial isolates obtained. High levels of AMR to several antimicrobial classes have been reported, including to β-lactams, glycopeptides, tetracyclines, aminoglycosides, sulphonamides, and quinolones. Multi-resistant strains, some relevant to human and veterinary medicine like extended-spectrum β-lactamase-producing E. coli and vancomycin-resistant enterococci, have been repeatedly isolated even in non-synanthropic animal species. Of particular relevance are reports of AMR bacteria in wildlife from natural reserves and endangered species. Future work awaits as this threatening yet unsolved problem persists.GRAPHICAL ABSTRACTSummary diagram of the antimicrobial resistance surveillance work developed by the UTAD Functional Genomics and Proteomics Unit.

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“…In Europe, the major reservoir 82 of VRE was initially present in the healthy human population, 83 2-10% of whom were carriers [8]. The use of avoparcin, a 84 glycopeptide that induces cross-resistance to vancomycin, as a 85 growth promoter in food animals during the 1980s, is thought to 86 be the reason for the expansion of the VRE community reservoir 87 [1,2,8]. 88 In the European Union, the majority of antibiotic classes used 89 in veterinary medicine are also used in human medicine, and 90 only a few antibiotic classes, like glycopeptides, oxazolidinones 91 and carbapenems, are reserved only for human therapy [6].…”

mentioning

confidence: 99%

“…More We chose to study the strain SU18, a VREfm, previously 147 characterized and recovered from faecal samples of pigs 148 slaughtered for human consumption [1]. A preculture was 149 set up from 2 or 3 individual colonies and grown in brain heart 150 infusion broth, at 37°C with shaking at 150 rpm, overnight.…”

mentioning

confidence: 99%

Effect of vancomycin on the proteome of the multiresistant Enterococcus faecium SU18 strain

Ramos

Chafsey

Silva

et al. 2015

Journal of Proteomics

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Genetic characterisation of antibiotic resistance and virulence factors in vanA-containing enterococci from cattle, sheep and pigs subsequent to the discontinuation of the use of avoparcin

Cited by 26 publications

References 9 publications

Capacity of Listeria monocytogenes Strains from the 2011 Cantaloupe Outbreak To Adhere, Survive, and Grow on Cantaloupe

Capacity of Listeria monocytogenes Strains from the 2011 Cantaloupe Outbreak To Adhere, Survive, and Grow on Cantaloupe

A Decade-Long Commitment to Antimicrobial Resistance Surveillance in Portugal

Effect of vancomycin on the proteome of the multiresistant Enterococcus faecium SU18 strain

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