Genetic Biofortification to Enrich Rice and Wheat Grain Iron: From Genes to Product

Ludwig, Yvonne; Slamet‐Loedin, Inez H.

doi:10.3389/fpls.2019.00833

Cited by 93 publications

(50 citation statements)

References 94 publications

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“…Similar OE lines with different genetic backgrounds (Swarna, IR68144, BR29, IR64, M12) were constructed using distinct promoters (OsGluB1, OsGtbl, CluB1, GluB4, OsG1b), leading to stable lines [115]. Other genes have been used as targets to increase Fe content in rice, thus the ferritin gene (OsFer2) involved in Fe transport, OE of OsYSL and NAS gene subfamilies involved in Fe uptake, were also overexpressed to produce Fe enriched lines [116].…”

Section: Mineral Seed Biofortification To Cope With Nutrient Deficienmentioning

confidence: 99%

The Importance of Ion Homeostasis and Nutrient Status in Seed Development and Germination

et al. 2020

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Seed is the dissemination unit of plants initiating an important stage in the life cycle of plants. Seed development, comprising two phases: embryogenesis and seed maturation, may define the quality of sown seed, especially under abiotic stress. In this review we have focused on the recent advances in the molecular mechanisms underlying these complex processes and how they are controlled by distinct environmental factors regulating ion homeostasis into the seed tissues. The role of transporters affecting seed embryogenesis and first stages of germination as imbibition and subsequent radicle protrusion and extension were revised from a molecular point of view. Seed formation depends on the loading of nutrients from the maternal seed coat to the filial endosperm, a process of which the efflux is not clear and where different ions and transporters are involved. The clear interrelation between soil nutrients, presence of heavy metals and the ion capacity of penetration through the seed are discussed in terms of ion effect during different germination stages. Results concerning seed priming techniques used in the improvement of seed vigor and radicle emergence are shown, where the use of nutrients as a novel way of osmopriming to alleviate abiotic stress effects and improve seedlings yield is discussed. Novel approaches to know the re-translocation from source leaves to developing seeds are considered, as an essential mechanism to understand the biofortification process of certain grains in order to cope with nutrient deficiencies, especially in arid and semiarid areas. Finally, the role of new genes involved in hormone-dependent processes, oxidative response and water uptake into the seeds during their development or germination, have been described as plant mechanisms to deal with abiotic stresses.

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Section: Mineral Seed Biofortification To Cope With Nutrient Deficienmentioning

confidence: 99%

The Importance of Ion Homeostasis and Nutrient Status in Seed Development and Germination

et al. 2020

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“…The genes OsFDH (Formate dehydrogenase), OsGSTU (Glutathione -S-transferase), OsPDR (Pleotropic Drug Resistance) are involved in metal homeostasis especially in iron acquisition and also stress tolerance in rice plants (Narayanan et al, 2007). These genes are involved in uptake, translocation, and storage of iron, zinc and other micronutrients in rice plants (Ludwig et al, 2019). In a study the 9 metal related genes OsYSL6, OsYSL8, OsYSL14, OsNRAMP1,OsNRAMP7, OsNRAMP8, OsNAS1, OsFRO1 and OsNAC5 were specifically overexpressed in flag leaves of rice and showed significant correlation with grain iron and zinc concentrations in seeds (Sperotto et al,2010).…”

Section: Resultsmentioning

confidence: 99%

SSR markers for grain iron zinc and yield-related traits polymorphic between Samba Mahsuri (BPT5204) and a wild rice Oryza rufipogon

Chandu

Addanki

Balakrishnan

et al. 2020

EJPB

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Identification of molecular markers revealing polymorphism among the parental lines are prerequisite for mapping QTLs and genes for desired traits. The genomic regions which contributes to the accumulation of grain iron and zinc in rice could greatly help in rice bio fortification programs. A BC 4 F 10 mapping population was earlier developed from the cross between an elite fine-grain Oryza sativa indica cultivar, BPT5204 and a wild progenitor specie O. rufipogon WR119. A total of 800 randomly selected SSR markers distributed on all the 12 chromosomes of rice including 50 gene specific markers related to grain iron, zinc and yield traits were used to identify the polymorphic loci between the two genotypes. In all, 166 markers (20.75 %) showed distinct polymorphism. 149 SSR markers (19%) out of 750 SSRs and 17 out of 50 gene-specific markers (36%) were polymorphic. The 17 polymorphic gene-specific markers were related to gene families OsZIP, OsYSL, OsNRAMP, OsNAAT, OsFRO, OsFDH, OsGSTU and OsPDR which are involved in metal transport and homeostasis in rice. Among the markers reported to be significantly associated with QTLs for grain iron, zinc and yield related traits, RM517, RM81A, RM264, OsYSL-7, RM5460, RM3874 were polymorphic in this study.

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“…Deficiency of iron is most common in the human population which leads to some important diseases such as anemia, impaired development and growth in preschool children and pregnant woman (World Health Organization [WHO], 2017). This crucial element plays a key role in electron transfer in both photosynthesis and respiration (Inoue et al, 2009;Ludwig and Slamet-Loedin, 2019). Biofortification of rice involves iron uptake, translocation and storage (Takagi et al, 1984).…”

Section: Manipulation Of Iron and Zinc Contentmentioning

confidence: 99%

Genetic Manipulation for Improved Nutritional Quality in Rice

2020

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Food with higher nutritional value is always desired for human health. Rice is the prime staple food in more than thirty developing countries, providing at least 20% of dietary protein, 3% of dietary fat and other essential nutrients. Several factors influence the nutrient content of rice which includes agricultural practices, post-harvest processing, cultivar type as well as manipulations followed by selection through breeding and genetic means. In addition to mutation breeding, genetic engineering approach also contributed significantly for the generation of nutrition added varieties of rice in the last decade or so. In the present review, we summarize the research update on improving the nutritional characteristics of rice by using genetic engineering and mutation breeding approach. We also compare the conventional breeding techniques of rice with modern molecular breeding techniques toward the generation of nutritionally improved rice variety as compared to other cereals in areas of micronutrients and availability of essential nutrients such as folate and iron. In addition to biofortification, our focus will be on the efforts to generate low phytate in seeds, increase in essential fatty acids or addition of vitamins (as in golden rice) all leading to the achievements in rice nutrition science. The superiority of biotechnology over conventional breeding being already established, it is essential to ascertain that there are no serious negative agronomic consequences for consumers with any difference in grain size or color or texture, when a nutritionally improved variety of rice is generated through genetic engineering technology.

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Genetic Biofortification to Enrich Rice and Wheat Grain Iron: From Genes to Product

Cited by 93 publications

References 94 publications

The Importance of Ion Homeostasis and Nutrient Status in Seed Development and Germination

The Importance of Ion Homeostasis and Nutrient Status in Seed Development and Germination

SSR markers for grain iron zinc and yield-related traits polymorphic between Samba Mahsuri (BPT5204) and a wild rice Oryza rufipogon

Genetic Manipulation for Improved Nutritional Quality in Rice

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