Genetic bioaugmentation as an effective method for in situ bioremediation: Functionality of catabolic plasmids following conjugal transfers

Ikuma, Kaoru; Gunsch, Claudia K.

doi:10.4161/bbug.20551

Cited by 9 publications

(24 citation statements)

References 5 publications

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“…Given the possibilities of plasmid exchange among strains, bioaugmentation in catabolic genes, not in bacteria, has taken relevance in the past decade (170). This approach is called "genetic bioaugmentation" and involves the introduction of bacteria harboring a relevant catabolic self-transmissible plasmid that stimulates the horizontal gene transfer of the plasmids into indigenous microorganisms with better fitness for survival in the corresponding niche (171). To design an optimal genetic bioaugmentation is important to choose the appropriate type of plasmid, to study the transfer capacity of the microorganism and the stability of the plasmid in the new bacteria, as well as to study the expression of the catabolic genes (171,172).…”

Section: Genomic Bioaugmentation As a Biotechnological Applicationmentioning

confidence: 99%

“…This approach is called "genetic bioaugmentation" and involves the introduction of bacteria harboring a relevant catabolic self-transmissible plasmid that stimulates the horizontal gene transfer of the plasmids into indigenous microorganisms with better fitness for survival in the corresponding niche (171). To design an optimal genetic bioaugmentation is important to choose the appropriate type of plasmid, to study the transfer capacity of the microorganism and the stability of the plasmid in the new bacteria, as well as to study the expression of the catabolic genes (171,172). Plasmid stability in the recipient strains may impact the effectiveness of bioremediation; highly stable plasmids could be necessary to clean up sites that continuously receive contaminant input (171).…”

Section: Genomic Bioaugmentation As a Biotechnological Applicationmentioning

confidence: 99%

“…To design an optimal genetic bioaugmentation is important to choose the appropriate type of plasmid, to study the transfer capacity of the microorganism and the stability of the plasmid in the new bacteria, as well as to study the expression of the catabolic genes (171,172). Plasmid stability in the recipient strains may impact the effectiveness of bioremediation; highly stable plasmids could be necessary to clean up sites that continuously receive contaminant input (171). Horizontal gene transfer from donor to indigenous bacteria following the deliberate release of phenol-degrading laboratory bacteria was shown to be important in the degradation of phenol in river waters continuously polluted by phenolic compounds (91).…”

Section: Genomic Bioaugmentation As a Biotechnological Applicationmentioning

confidence: 99%

See 2 more Smart Citations

Plasmid-Mediated Tolerance Toward Environmental Pollutants

Segura

Molina²,

Ramos

2014

Microbiol Spectr

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The survival capacity of microorganisms in a contaminated environment is limited by the concentration and/or toxicity of the pollutant. Through evolutionary processes, some bacteria have developed or acquired mechanisms to cope with the deleterious effects of toxic compounds, a phenomenon known as tolerance. Common mechanisms of tolerance include the extrusion of contaminants to the outer media and, when concentrations of pollutants are low, the degradation of the toxic compound. For both of these approaches, plasmids that encode genes for the degradation of contaminants such as toluene, naphthalene, phenol, nitrobenzene, and triazine or are involved in tolerance toward organic solvents and heavy metals, play an important role in the evolution and dissemination of these catabolic pathways and efflux pumps. Environmental plasmids are often conjugative and can transfer their genes between different strains; furthermore, many catabolic or efflux pump genes are often associated with transposable elements, making them one of the major players in bacterial evolution. In this review, we will briefly describe catabolic and tolerance plasmids and advances in the knowledge and biotechnological applications of these plasmids.

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Section: Genomic Bioaugmentation As a Biotechnological Applicationmentioning

confidence: 99%

Section: Genomic Bioaugmentation As a Biotechnological Applicationmentioning

confidence: 99%

Section: Genomic Bioaugmentation As a Biotechnological Applicationmentioning

confidence: 99%

See 1 more Smart Citation

Plasmid-Mediated Tolerance Toward Environmental Pollutants

Segura

Molina²,

Ramos

2014

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

“…To decrease the time required for the agent to cross the membrane, recombinant strains that can express the organophosphorous hydrolase on the surface of the cells [40,41] and secrete it into the periplasmic space [42] were constructed. As another method, a bacterium carrying a high functionality catabolic plasmid was also constructed, which showed the ability to perform conjugal transfers [43]. When this bacterium is present in the soil, the degradation ability can be increased by conjugal transfer.…”

Section: Enhancement the Performance Of Degradation And Stability Usimentioning

confidence: 99%

A Novel Bioremediation Method for Shallow Layers of Soil Polluted by Pesticides

Shiomi¹

2013

Applied Bioremediation - Active and Passive Approaches

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“…Studies are also concerned with the investigation of individual genetic determinants of natural bacterial degraders. Since many genetic determinants of the biodegradation of aromatic compounds are localized within extra-chromosomal genetic elements, the plasmid composition of bacterial degraders and the effect of bacteria containing biodegradation plasmids on the utilization of oil and its products are continuously studied [36][37][38][39][40][41][42][43].…”

Section: Introductionmentioning

confidence: 99%

Salicylate or Phthalate: The Main Intermediates in the Bacterial Degradation of Naphthalene

Travkin

Solyanikova

2021

Processes

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Polycyclic aromatic hydrocarbons (PAHs) are widely presented in the environment and pose a serious environmental threat due to their toxicity. Among PAHs, naphthalene is the simplest compound. Nevertheless, due to its high toxicity and presence in the waste of chemical and oil processing industries, naphthalene is one of the most critical pollutants. Similar to other PAHs, naphthalene is released into the environment via the incomplete combustion of organic compounds, pyrolysis, oil spills, oil processing, household waste disposal, and use of fumigants and deodorants. One of the main ways to detoxify such compounds in the natural environment is through their microbial degradation. For the first time, the pathway of naphthalene degradation was investigated in pseudomonades. The salicylate was found to be a key intermediate. For some time, this pathway was considered the main, if not the only one, in the bacterial destruction of naphthalene. However, later, data emerged which indicated that gram-positive bacteria in the overwhelming majority of cases are not capable of the formation/destruction of salicylate. The obtained data made it possible to reveal that protocatechoate, phthalate, and cinnamic acids are predominant intermediates in the destruction of naphthalene by rhodococci. Pathways of naphthalene degradation, the key enzymes, and genetic regulation are the main subjects of the present review, representing an attempt to summarize the current knowledge about the mechanism of the microbial degradation of PAHs. Modern molecular methods are also discussed in the context of the development of “omics” approaches, namely genomic, metabolomic, and proteomic, used as tools for studying the mechanisms of microbial biodegradation. Lastly, a comprehensive understanding of the mechanisms of the formation of specific ecosystems is also provided.

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Genetic bioaugmentation as an effective method for in situ bioremediation: Functionality of catabolic plasmids following conjugal transfers

Cited by 9 publications

References 5 publications

Plasmid-Mediated Tolerance Toward Environmental Pollutants

Plasmid-Mediated Tolerance Toward Environmental Pollutants

A Novel Bioremediation Method for Shallow Layers of Soil Polluted by Pesticides

Salicylate or Phthalate: The Main Intermediates in the Bacterial Degradation of Naphthalene

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