Genetic behavior of induced translocation heterozygote in Artemisia annua L.

Abstract

From the population raised from gamma irradiated seeds at different doses (100, 200 and 300 Gy), at 100 Gy phenotypically dissimilar plant (from the control plants) was isolated. The plant was shorter and morphologically weaker in contrast to control population. The cytological studies were carried out in a plant showed segmental exchanges between heterologous chromosomes. Chromosomal associations in rings and chains along with bivalents and univalents were observed in pollen mother cells (PMCs) during microsporogenesis. Various anaphasic anomalies were also taken into account including chromatid bridges, laggards and unequal separations etc. Subsequent stages of meiosis were also found to be highly intermittent. Owing to the high frequency of chromosomal aberrations, pollen fertility in control (96.32%) were efficaciously reduced to 38.26% at dose of 100Gy. The present study has been conducted to assess cytological behavior of the induced translocation heterozygote which is first time induced through gamma irradiation in the Artemisia annua L.