Genetic basis of penicillin resistance of S. aureus isolated in bovine mastitis

Bağcıgil, Arzu Funda; Taponen, Suvi; Koort, Joanna; Bengtsson, Björn; Myllyniemi, Anna-Liisa; Pyörälä, Satu

doi:10.1186/1751-0147-54-69

Cited by 32 publications

(29 citation statements)

References 28 publications

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“…In fact, the largest pulsotype in this study, pulsotype U was susceptible to penicillin, an indication that penicillin resistance was pulsotype dependent. Interestingly, a link between penicillin susceptibility and pulsotypes has been reported (Bagcigil et al 2012). A multi-resistance phenotype was observed in 2 (2·5%) isolates.…”

Section: Resultsmentioning

confidence: 98%

Characterisation ofStaphylococcus aureusstrains isolated from mastitis bovine milk in Argentina

Srednik

Usongo

Lépine

et al. 2018

Journal of Dairy Research

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The study reported in this Research Communication was conducted to characterise Staphylococcus aureus isolates recovered from mastitic bovine milk from dairy herds in Argentina. A total of 829 mastitic milk samples, both clinical and subclinical, were collected from 21 farms by veterinarians and submitted to the laboratory for testing from which 229 S. aureus isolates were recovered, an isolation rate of 28·1%. These isolates were tested for susceptibility to the antibiotics penicillin, erythromycin and clindamycin. Of the 229 isolates, 53 (23·1%) were resistant to penicillin, 31 (13·5%) to erythromycin and 28 (12·2%) to clindamycin. All isolates were negative for the mecA, mecC and pvl genes by PCR. Southernblot hybridisation revealed that the ermC gene was located on plasmid bands. Eighty isolates were randomly selected from the 229 for further characterisation. Restriction analysis of chromosomal DNA with Cf9I followed by PFGE of the 80 isolates revealed 23 distinct pulsotypes at 80% similarity. Seven major types (A, B, N, P, S, T, U and V) accounted for 68·7% of these isolates and 12 pulsotypes (A, B, F, G, J, K, M, N, P, S, T and U) occurred on more than one farm indicating genetic diversity within the farms. MLST of a representative isolate from dominant types identified the STs 97 705, 746, 2102 and 2187 with ST97 being the most predominant. Antibiotic susceptibility testing showed that 53·7% of the 80 randomly selected isolates were resistant to at least one of the three antibiotics tested. To our knowledge, this study represents the first large scale molecular studies on S. aureus isolates from dairy farms in Argentina.

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Section: Resultsmentioning

confidence: 98%

Characterisation ofStaphylococcus aureusstrains isolated from mastitis bovine milk in Argentina

Srednik

Usongo

Lépine

et al. 2018

Journal of Dairy Research

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“…Both blaZ and tetK were highly prevalent in S. epidermidis , whereas msrA was common in S. arlettae, S. cohnii , and S. equorum . These genes have been detected in NAS isolated from dairy cows (Bagcigil et al, 2012 ; Li et al, 2015 ; Bochniarz et al, 2016 ). Their dissemination undermines therapeutic options, since β-lactams, macrolides and tetracyclines are among the most commonly used antimicrobials in Canadian dairy herds (Saini et al, 2012a ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Genetic Basis of Antimicrobial Resistance in Non-aureus Staphylococci Isolated from Canadian Dairy Herds

et al. 2018

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Emergence and spread of antimicrobial resistance is a major concern for the dairy industry worldwide. Objectives were to determine: (1) phenotypic and genotypic prevalence of drug-specific resistance for 25 species of non-aureus staphylococci, and (2) associations between presence of resistance determinants and antimicrobial resistance. Broth micro-dilution was used to determine resistance profiles for 1,702 isolates from 89 dairy herds. Additionally, 405 isolates were sequenced to screen for resistance determinants. Antimicrobial resistance was clearly species-dependent. Resistance to quinupristin/dalfopristin was common in Staphylococcus gallinarum (prevalence of 98%), whereas S. cohnii and S. arlettae were frequently resistant to erythromycin (prevalence of 63 and 100%, respectively). Prevalence of resistance was 10% against β-lactams and tetracyclines. In contrast, resistance to antimicrobials critically important for human medicine, namely vancomycin, fluoroquinolones, linezolid and daptomycin, was uncommon (< 1%). Genes encoding multidrug-resistance efflux pumps and resistance-associated residues in deducted amino acid sequences of the folP gene were the most frequent mechanisms of resistance, regardless of species. The estimated prevalence of the mecA gene was 17% for S. epidermidis. Several genes, including blaZ, mecA, fexA, erm, mphC, msrA, and tet were associated with drug-specific resistance, whereas other elements were not. There were specific residues in gyrB for all isolates of species intrinsically resistant to novobiocin. This study provided consensus protein sequences of key elements previously associated with resistance for 25 species of non-aureus staphylococci from dairy cattle. These results will be important for evaluating effects of interventions in antimicrobial use in Canadian dairy herds.

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“…Mastitis-causing streptococcal species have remained susceptible to benzylpenicillin (Pitkälä et al, 2004;Hendriksen et al, 2008;Bengtsson et al, 2009;Kalmus et al, 2011). Staphylococcus aureus and CNS isolated from bovine mastitis have developed resistance to penicillin (Hendriksen et al, 2008;Bagcigil et al, 2012), which may significantly influence the efficacy of treatment (Pyörälä and Pyörälä 1998;Sol et al;Taponen et al, 2003b). In our study, 6 of 8 cases of mastitis caused by penicillin-susceptible Staph.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of 5-day parenteral versus intramammary benzylpenicillin for treatment of clinical mastitis caused by gram-positive bacteria susceptible to penicillin in vitro

Kalmus

Simojoki

Orro

et al. 2014

Journal of Dairy Science

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The efficacy of parenteral (intramuscular) or intramammary (IMM) benzylpenicillin treatment for clinical mastitis caused by gram-positive bacteria susceptible to penicillin in vitro was investigated. Cows with clinical mastitis in 1 udder quarter were randomly placed into 2 treatment groups. The preliminary bacteriological diagnosis of intramammary infection (IMI) was based on on-farm culturing, and the bacteriological diagnoses were later confirmed by a quantitative PCR assay. Clinical mastitis caused by gram-positive bacteria susceptible to benzylpenicillin was treated with penicillin via either the parenteral route (20mg/kg) or IMM route (600mg) once per day for 5d. The outcome of the treatment was evaluated 3 to 4wk after the onset of the treatment. The affected quarter was examined to assess the clinical cure, and milk samples were collected from the affected quarter to determine the bacteriological cure and milk N-acetyl-β-d-glucosaminidase activity. The survival and the composite milk somatic cell counts of the treated cows were followed up for 6 and 3mo after treatment, respectively. A total of 140 cows with clinical mastitis were included in the study, 61 being treated with benzylpenicillin parenterally and 79 via the IMM route. From all quarters treated, 108 of 140 (77.1%) were cured clinically and 77 of 140 (55.0%) were cured bacteriologically. The route of treatment did not significantly affect the outcome of the treatment; 80.3% of the quarters with parenteral treatment and 74.7% of the quarters with IMM treatment showed a clinical cure, and 54.1 and 55.7% a bacteriological cure, respectively. The milk N-acetyl-β-d-glucosaminidase activity was significantly lower in the quarters with a clinical or bacteriological cure than in the quarters with no cure. The 6-mo survival and the proportion of cows with composite milk somatic cell counts <200,000/mL among the treated cows during the 3-mo follow-up period did not significantly differ between the treatment groups. In conclusion, the outcome of either parenteral or IMM benzylpenicillin treatment of clinical mastitis caused by penicillin-susceptible bacteria was similar.

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Genetic basis of penicillin resistance of S. aureus isolated in bovine mastitis

Cited by 32 publications

References 28 publications

Characterisation ofStaphylococcus aureusstrains isolated from mastitis bovine milk in Argentina

Characterisation ofStaphylococcus aureusstrains isolated from mastitis bovine milk in Argentina

Prevalence and Genetic Basis of Antimicrobial Resistance in Non-aureus Staphylococci Isolated from Canadian Dairy Herds

Efficacy of 5-day parenteral versus intramammary benzylpenicillin for treatment of clinical mastitis caused by gram-positive bacteria susceptible to penicillin in vitro

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