Genetic Association of LEP Gene Polymorphisms with Obesity in Moroccan Individuals: Case–Control Study and Updated Meta-analysis

Bouafi, Hind; Krami, Al Mehdi; Morjane, Imane; Slaoui, Kenza; Harmak, Houda; Charoute, Hicham; Saı̈le, Rachid; Barakat, Abdelhamid

doi:10.1007/s10528-023-10342-8

Cited by 3 publications

(1 citation statement)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is a study of Bouafi et al (2023) indicating an association between the LEP gene polymorphisms rs7799039 and rs11761556 with obesity in Moroccan individuals. No significant association was found between the polymorphisms rs7799039 and rs11761556 and the risk of obesity.…”

Section: Discussionmentioning

confidence: 99%

GHRL, LEP, LEPR genes polymorphism and their association with the metabolic syndrome in the Ukrainian population

Prodan,

Dzubanovsky,

Kamyshnyi

et al. 2023

Endocrine Regulations

View full text Add to dashboard Cite

Objective. Many conflicting results have been obtained in the study of leptin (LEP) and leptin receptor (LEPR) gene variants that are associated with the obesity and diabetes possibly due to differences in the study populations. The aim of this study was to evaluate changes in the metabolic hormones (leptin, ghrelin, adiponectin, resistin) levels in the blood of obese patients in relation to the GHRL (rs696217), LEP (rs7799039), LEPR (rs1137100, rs1137101, rs1805094) polymorphism in Ukrainian population. Methods. The study involved 53 obesity cases and 48 non-obesity subjects (controls). The GHRL, LEP, and LEPR genes polymorphism (rs696217, rs7799039, rs1137100, rs1137101, rs1805094) was genotyped using a TaqMan real-time polymerase chain reaction method. Blood hormones (leptin, ghrelin, adiponectin, resistin) were determined with commercially available kits using a Multiskan FC analyzer. Results. The study of the effect of genotypes of the GHRL (rs696217), LEP (rs7799039), and LEPR (rs1137100, rs1805094) polymorphisms on the level of metabolic hormones (leptin, ghrelin, adiponectin, resistin) in the blood of obese patients did not show reliably significant results. Thus, the presence of the LEPR genes (rs1137101) polymorphism in the Ukrainian population indicates an increased risk of the metabolic syndrome development regardless of the homozygous or heterozygous genotype (genotypes AA, AG, GG). Conclusions. We established a significant effect of the presence of the A allele and G allele of the LEPR gene polymorphism (rs1137101) on the level of leptin, ghrelin, adiponectin, and resistin in the serum of patients diagnosed with the metabolic syndrome in the Ukrainian population.

show abstract

Section: Discussionmentioning

confidence: 99%

GHRL, LEP, LEPR genes polymorphism and their association with the metabolic syndrome in the Ukrainian population

Prodan,

Dzubanovsky,

Kamyshnyi

et al. 2023

Endocrine Regulations

View full text Add to dashboard Cite

show abstract

Leptin Rs7799039 polymorphism is associated with type 2 diabetes mellitus Egyptian patients

Mohamed,

Abo-Elmatty,

Wahba

et al. 2023

Archives of Physiology and Biochemistry

View full text Add to dashboard Cite

Association of leptin–melanocortin gene polymorphisms with the risk of obesity in northwest Indian population

Sharma,

Badaruddoza

2024

Egypt J Med Hum Genet

View full text Add to dashboard Cite

Background Obesity, a multifaceted endocrine issue, is adversely affecting all age groups and is posing a significant public health challenge. The genetic polymorphisms of the melanocortin 4 receptor (MC4R) and leptin (LEP) genes likely contribute to the development of obesity. The present study aimed to explore the effects of MC4R and LEP gene polymorphisms on obesity among the northwest Indian population. Methods The present study was conducted among 333 obese cases and 338 non-obese controls (aged 18–50 years). All subjects underwent measurements for anthropometric, physiometric, as well as biochemical parameters. Genotyping for MC4R and LEP gene variants was performed using the polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) method. However, 10% of the samples for each variant were confirmed using the Sanger sequencing method. Results The polymorphisms of leptin–melanocortin pathway genes (MC4R-LEP) were found to be significantly associated with various obesity-related parameters like waist circumference: p = 0.017, waist-to-height ratio: p = 0.009, total cholesterol: p = 0.0001 and triglycerides: p = 0.0001. Both the LEP gene variants rs2167270 and rs7799039 conferred 2.4- and 2.2-fold risk toward obesity under the recessive genetic model [OR (95% CI) 2.42 (1.44–4.07), p = 0.001; OR (95% CI) 2.26 (1.41–3.60), p = 0.0001, respectively]. All four polymorphisms of the MC4R and LEP genes demonstrated a strong interaction of 82.1% with the lifestyle factor (p = 0.001). The haplotype combinations A–A for rs571312 and rs12970134 conferred twofold risk [OR (95% CI) 2.61 (1.10–6.20), p = 0.028]. However, the combination A–G for rs2167270 and rs7799039 predicted sixfold risk [OR (95% CI) 6.02 (3.39–10.68), p = 0.0001] toward the obesity development in this population. Conclusion Our study revealed a connection between MC4R (rs571312, rs12970134) and LEP (rs2167270, rs7799039) gene variants with obesity, highlighting their prominent role in assessing the risk of obesity among the northwest Indian population.

show abstract

Genetic Association of LEP Gene Polymorphisms with Obesity in Moroccan Individuals: Case–Control Study and Updated Meta-analysis

Cited by 3 publications

References 57 publications

GHRL, LEP, LEPR genes polymorphism and their association with the metabolic syndrome in the Ukrainian population

GHRL, LEP, LEPR genes polymorphism and their association with the metabolic syndrome in the Ukrainian population

Leptin Rs7799039 polymorphism is associated with type 2 diabetes mellitus Egyptian patients

Association of leptin–melanocortin gene polymorphisms with the risk of obesity in northwest Indian population

Contact Info

Product

Resources

About