Genetic Association of Angiotensin-converting enzyme 2 ACE-2 (rs2285666) Polymorphism with the Susceptibility of COVID-19 Disease in Iraqi Patients

doi:10.26538/tjnpr/v7i2.7

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…It enhances the entry of the virus into the cell, causing the final infection [ 6 , 7 ], and alters host susceptibility to SARS-CoV-2, reflecting ACE-2 spike glycoprotein interaction [ 8 ]. Genetic polymorphisms that control ACE-2 expression have the potential to significantly alter COVID-19 severity and host cell response [ 9 , 10 ]. Several SNPs were investigated in this context, including rs182366225, rs2097723, rs1027571965, and rs2285666.…”

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confidence: 99%

Angiotensin-Converting Enzyme-2 (ACE-2) with Interferon-Induced Transmembrane Protein-3 (IFITM-3) Genetic Variants and Interleukin-6 as Severity and Risk Predictors among COVID-19 Egyptian Population

Makled,

Ali,

Eldahdouh

et al. 2023

International Journal of Microbiology

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Introduction. The host genetic background is a crucial factor that underlies the interindividual variability of COVID-19 fatality and outcomes. Angiotensin-converting enzyme-2 (ACE-2) and interferon-induced transmembrane protein-3 (IFITM-3) have a key role in viral cell entrance and priming. The evoked immune response will also provide a predictive prognosis for COVID-19 infection. This study aimed to explore the association between ACE-2 and IFITM-3 genotypes and their corresponding allele frequencies with disease severity indices in the Egyptian COVID-19 population. The serum level of interleukin-6, as a biomarker of hyperinflammatory response, and cytokine storm, was correlated with disease progression, single nucleotide polymorphisms (SNPs) of the selected receptors, and treatment response. Methodology. We enrolled 900 COVID-19-confirmed cases and 100 healthy controls. Genomic DNA was extracted from 200 subjects (160 patients selected based on clinical and laboratory data and 40 healthy controls). The ACE-2 rs2285666 and IFITM-3 rs12252 SNPs were genotyped using the TaqMan probe allelic discrimination assay, and the serum IL-6 level was determined by ELISA. Logistic regression analysis was applied to analyze the association between ACE-2 and IFITM-3 genetic variants, IL-6 profile, and COVID-19 severity. Results. The identified genotypes and their alleles were significantly correlated with COVID-19 clinical deterioration as follows: ACE2 rs2285666 CT + TT, odds ratio (95% confidence interval): 12.136 (2.784–52.896) and IFITM-3 rs12252 AG + GG: 17.276 (3.673–81.249), both p<0.001. Compared to the controls, the heterozygous and mutant genotypes for both SNPs were considerable risk factors for increased susceptibility to COVID-19. IL-6 levels were significantly correlated with disease progression (p<0.001). Conclusion. ACE-2 and IFITM-3 genetic variants are potential predictors of COVID-19 severity, critical outcomes, and post-COVID-19 complications. Together, these SNPs and serum IL-6 levels explain a large proportion of the variability in the severity of COVID-19 infection and its consequences among Egyptian subjects.

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Section: Introductionmentioning

confidence: 99%

Angiotensin-Converting Enzyme-2 (ACE-2) with Interferon-Induced Transmembrane Protein-3 (IFITM-3) Genetic Variants and Interleukin-6 as Severity and Risk Predictors among COVID-19 Egyptian Population

Makled,

Ali,

Eldahdouh

et al. 2023

International Journal of Microbiology

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Bioremediation by bacteria isolated from water contaminated with hydrocarbons

Fahim Muhsin,

Fadhil AboKsour,

Hadi

2023

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Oil pollution is currently a global problem. However, an oil-contaminated ecology is rich in microorganisms that may utilize petroleum oil and hydrocarbons for growth, feeding, and metabolic processes. In the present study, fifty polluted water samples were collected from five stations (ten samples each) in the Al-Fahama oil refinery in eastern Baghdad. The water contamination parameters of these collected water samples were detected. Then, the percentage of water contamination with some heavy metals (zinc, lead, and cadmium) and radioactive elements (uranium, cesium and actinium) was measured. The proportions of these elements were compared within their limits permitted by the World Health Organization (WHO). Fifty-nine bacterial isolates were isolated from polluted water, and 24 isolates of them succeeded in analyzing crude oil. The results of the current study showed that seven isolates belong to the genus Citrobacter amalonaticus (29.16%), six isolates belong to Enterobacter cloacae (25%), three isolates belonged to both Pseudomonas aeruginosa (12.5%) and Ochrobacterum anthropi (12.5%), and human Ochrobacterum. With a percentage of 12.5%, two isolates of Serratia marcescens (8.3%) and one isolate of each Pseudomonas fluorescens, Serratia fonticola, and Burkholderia pseudomallei (4.16%) of each. The optimum of some conditions for the decomposition process was determined in terms of (pH, temperature and crude oil concentration) and the results showed that the optimum degradation conditions were 35°C at pH equal to 7.5 in the presence of 2% of crude oil. Several experiments were conducted to determine the most efficient isolates for oil analysis. Burkholderia pseudomallei and Pseudomonas fluorescens are the most active bacterial species in their oil degradation. Genes responsible for hydrocarbon analysis were revealed in twenty-four bacterial isolates using a polymerase chain reaction (PCR) assay. The results showed that the ALKB gene (alkane hydroxylase) was observed in all bacterial isolates that succeeded in analyzing crude oil with a percentage equal to 100%, NahAc gene (naphthalene dioxygenase) has been recorded in four isolates (16.7%), these four bacterial isolates were Burkholderia pseudomallei, Pseudomonas aeruginosa, Ochrobacterum anthropic, and Pseudomonas fluorescens. Generally, the isolation rate of both C. amalonaticus and E. cloacae isolates was higher than in other studies, which may be due to the hydrocarbon pollution in isolation; both B. pseudomallei and P. fluorescens isolates were the highest active bacterial species in their oil degradation. Genetic results showed that the AlkB gene was the domain compared with other degradation genes used in the current study, followed by NahAc gene. Keywords: Bioremediation, heavy metal, B. pseudomallei, hydrocarbons, crude oil

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The Immunohistochemical Staining of Vimentin and E-Cadherin in Bladder Cancer Patients Infected with Hepatitis C Virus

Ismael¹,

Qaddoori²,

Sha'ban³

et al. 2023

J. Pure Appl. Microbiol.

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The invasion and spread of cancer cells are two of the most notable characteristics of malignant tumors. Recent studies suggest that the epithelial-mesenchymal transition (EMT) has been linked to this significant occurrence. It is linked to the absence of the epithelial brow and the presence of mesenchymal facial hair. The aims of the present study were to explore the immunohistochemical staining of vimentin and E-cadherin ex vivo as EMT markers and assess their potential as predictive biomarkers for transitional cell cancer (TCC). In this study, 55 paraffin-embedded biopsies from TCC patients and 10 autopsies that appeared to be normal were included. Immunohistochemistry was used to produce patterns of vimentin and E-cadherin expression. When compared to female patients, the expression of E-cadherin and vimentin significantly increased with increasing age in male patients (> 50 years). In contrast to the considerable rise in vimentin expression in higher grades and stages of the tumor, E-cadherin expression was significantly reduced with tumor stage and grade. The findings of this study reveal that elevated vimentin and reduced E-cadherin are important indicators associated with a poor prognosis for TCC.

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Genetic Association of Angiotensin-converting enzyme 2 ACE-2 (rs2285666) Polymorphism with the Susceptibility of COVID-19 Disease in Iraqi Patients

Cited by 3 publications

References 38 publications

Angiotensin-Converting Enzyme-2 (ACE-2) with Interferon-Induced Transmembrane Protein-3 (IFITM-3) Genetic Variants and Interleukin-6 as Severity and Risk Predictors among COVID-19 Egyptian Population

Angiotensin-Converting Enzyme-2 (ACE-2) with Interferon-Induced Transmembrane Protein-3 (IFITM-3) Genetic Variants and Interleukin-6 as Severity and Risk Predictors among COVID-19 Egyptian Population

Bioremediation by bacteria isolated from water contaminated with hydrocarbons

The Immunohistochemical Staining of Vimentin and E-Cadherin in Bladder Cancer Patients Infected with Hepatitis C Virus

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