volume 66, issue 8, P5157-5160 1992
DOI: 10.1128/jvi.66.8.5157-5160.1992
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J Luban, K B Alin, K L Bossolt, T Humaran, S P Goff

Abstract: We have established a genetic assay for the multimerization of retroviral gag polyproteins. This assay is based on the GAL4 two-hybrid system for studying protein-protein interactions (S. Fields and 0. Song, Nature (London) 340:245-246, 1989). In our initial experiments, we generated Saccharomyces cerevisiae plasmids that separately express the GAL4 DNA-binding and GAL4 activation domains fused to the human immunodeficiency virus type 1 (HIV-1) gag polyprotein, Pr55w*. The coexpression of these two hybrid prot…

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