Genetic architecture of tipburn resistance in lettuce

Macias-González, M.; Truco, María José; Bertier, Lien; Jenni, Sylvie; Šimko, Ivan; Hayes, Ryan J.; Michelmore, Richard W.

doi:10.1007/s00122-019-03349-6

Cited by 26 publications

(36 citation statements)

References 47 publications

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“…To the best of our knowledge, this is the first report of the mapping of TB resistance in tomatoes. Major QTLs for TB have also been recently reported in lettuce (Jenni et al, 2013;Macias Gonzalez et al, 2019) however, no candidate gene has been suggested yet. The precise location of QTL or QTLs on chromosome 7 will allow an assessment of whether the genetic control on TB resistance could be similar in both species, as soon as candidate genes can be defined in posterior research studies.…”

Section: Discussionmentioning

confidence: 99%

Genetic Control of Reproductive Traits in Tomatoes Under High Temperature

Gonzalo

Chen

et al. 2020

Front. Plant Sci.

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Global climate change is increasing the range of temperatures that crop plants must face during their life cycle, giving negative effects to yields. In this changing scenario, understanding the genetic control of plant responses to a range of increasing temperature conditions is a prerequisite to developing cultivars with increased resilience. The current work reports the identification of Quantitative Trait Loci (QTL) involved in reproductive traits affected by temperature, such as the flower number (FLN) and fruit number (FRN) per truss and percentage of fruit set (FRS), stigma exsertion (SE), pollen viability (PV) and the incidence of the physiological disorder tipburn (TB).These traits were investigated in 168 Recombinant Inbred Lines (RIL) and 52 Introgression Lines (IL) derived from the cross between Solanum lycopersicum var. "MoneyMaker" and S. pimpinellifolium accession TO-937. Mapping populations were cultivated under increased temperature regimen conditions: T1 (25 • C day/21 • C night), T2 (30 • C day/25 • C night) and T3 (35 • C day/30 • C night). The increase in temperature drastically affected several reproductive traits, for example, FRS in Moneymaker was reduced between 75 and 87% at T2 and T3 when compared to T1, while several RILs showed a reduction of less than 50%. QTL analysis allowed the identification of genomic regions affecting these traits at different temperatures regimens. A total of 22 QTLs involved in reproductive traits at different temperatures were identified by multi-environmental QTL analysis and eight involved in pollen viability traits. Most QTLs were temperature specific, except QTLs on chromosomes 1, 2, 4, 6, and 12. Moreover, a QTL located in chromosome 7 was identified for low incidence of TP in the RIL population, which was confirmed in ILs with introgressions on chromosome 7. Furthermore, ILs with introgressions in chromosomes 1 and 12 had good FRN and FRS in T3 in replicated trials. These results represent a catalog of QTLs and pre-breeding materials that could be used as the starting point for deciphering the genetic control of the genetic response of reproductive traits at different temperatures and paving the road for developing new cultivars adapted to climate change.

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Section: Discussionmentioning

confidence: 99%

Genetic Control of Reproductive Traits in Tomatoes Under High Temperature

Gonzalo

Chen

et al. 2020

Front. Plant Sci.

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“…Elucidation of the effect of the decreased expression level of LsTCP4 is a future concern, but an excess of undulation induced in the leaf margin of the Empire type is probably the cause of tipburn under high-temperature conditions. It has been suggested that the major QTL for tipburn in LG5 has pleiotropic effects among multiple agricultural traits 4,5 . In addition, TCP family proteins in Arabidopsis interact with the FLOWERING LOCUS T protein 18,19 , which affects the flowering time 19 .…”

Section: Discussionmentioning

confidence: 99%

“…The serrated leaf margin in the Empire type is clearly associated with the high incidence of tipburn (Fig. 1b) 4,5 .…”

Section: Introductionmentioning

confidence: 99%

A CIN-like TCP transcription factor (LsTCP4) having retrotransposon insertion associates with a shift from Salinas type to Empire type in crisphead lettuce (Lactuca sativa L.)

et al. 2020

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To improve several agronomic traits in crisphead lettuce (Lactuca sativa L.) under high-temperature growth conditions, we investigated the correlation among those traits in multiple cultivars and performed genetic mapping of their causal genes. In a field cultivation test of Empire type (serrated leaf) and Salinas type (wavy leaf) cultivars, Empire type cultivars showed increased tipburn susceptibility and late bolting compared with Salinas type cultivars. We attempted genetic mapping of leaf shape and bolting time by ddRAD-seq using the F 2 population derived from a cross between 'VI185' (Empire type) and 'ShinanoGreen' (Salinas type). These analyses suggested that both traits are controlled by a single locus in LG5. Genotyping of 51 commercial lettuce cultivars with a tightly linked marker (LG5_v8_252.743Mbp) at this locus showed an association between its genotype and the serrated leaf phenotype. By further fine mapping and transcriptome analysis, a gene encoding putative CIN-like TCP transcription factor was determined to be a candidate gene at this locus and was designated as LsTCP4. An insertion of retrotransposable element was found in the allele of 'VI185', and its transcript level in the leaves was lower than that in 'ShinanoGreen'. Because shapes of leaf epidermal cells in 'VI185' were similar to those in the TCP family mutant of Arabidopsis thaliana, the leaf shape phenotype was likely caused by reduced expression of LsTCP4. Furthermore, because it is known that the TCP family protein also controls flowering time via interaction with FT in A. thaliana, it was highly possible that LsTCP4 gave pleiotropic effects on both leaf shape and bolting time in lettuce.

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“…Given the availability of markers in lettuce, Marker-Assisted Selection (MAS) has started to be adopted in plant breeding programmes for various purposes, including identification of resistance genes [13,14] or Quantitative Trait Loci (QTLs) of phytopathogens [15,16], the study of QTLs controlling complex traits [17,18], and investigation of the genetic identity and purity of either experimental or commercial lines [19]. On the other hand, very few attempts have been made to prove the efficiency of molecular markers in Marker-Assisted Breeding (MAB) activities, where the genotypic background is molecularly investigated to complement traditional phenotypic selection [20].…”

Section: Introductionmentioning

confidence: 99%

The Molecular Determination of Hybridity and Homozygosity Estimates in Breeding Populations of Lettuce (Lactuca sativa L.)

Patella

Palumbo

Galla

et al. 2019

Genes

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The development of new varieties of horticultural crops benefits from the integration of conventional and molecular marker-assisted breeding schemes in order to combine phenotyping and genotyping information. In this study, a selected panel of 16 microsatellite markers were used in different steps of a breeding programme of lettuce (Lactuca sativa L., 2 n = 18). Molecular markers were first used to genotype 71 putative parental lines and to plan 89 controlled crosses designed to maximise recombination potentials. The resulting 871 progeny plants were then molecularly screened, and their marker allele profiles were compared with the profiles expected based on the parental lines. The average cross-pollination success rate was 68 ± 33%, so 602 F1 hybrids were completely identified. Unexpected genotypes were detected in 5% of cases, consistent with this species’ spontaneous out-pollination rate. Finally, in a later step of the breeding programme, 47 different F3 progenies, selected by phenotyping for a number of morphological descriptors, were characterised in terms of their observed homozygosity and within-population genetic uniformity and stability. Ten of these populations had a median homozygosity above 90% and a median genetic similarity above 95% and are, therefore, particularly suitable for pre-commercial trials. In conclusion, this study shows the synergistic effects and advantages of conventional and molecular methods of selection applied in different steps of a breeding programme aimed at developing new varieties of lettuce.

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Genetic architecture of tipburn resistance in lettuce

Cited by 26 publications

References 47 publications

Genetic Control of Reproductive Traits in Tomatoes Under High Temperature

Genetic Control of Reproductive Traits in Tomatoes Under High Temperature

A CIN-like TCP transcription factor (LsTCP4) having retrotransposon insertion associates with a shift from Salinas type to Empire type in crisphead lettuce (Lactuca sativa L.)

The Molecular Determination of Hybridity and Homozygosity Estimates in Breeding Populations of Lettuce (Lactuca sativa L.)

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