Genetic appraisal of serological response post vaccination against enterotoxaemia (ET) in Malpura and Avikalin sheep

Abstract

Enterotoxaemia (ET) is a fatal enteric disease of small ruminants attributable to a toxigenic type of Clostridium perfringens. The key strategy for prevention of ET is the management and vaccination. Present study aimed at identifying the sources of variation for ET vaccine response especially against epsilon toxin in 173 sheep that included 83 Avikalin and 90 Malpura lambs raised at the institute flock in the semi-arid region of India. The mean age at vaccination was 90 days. Sera were tested by blocking ELISA. Study showed significant variability for response to ET vaccine. 5.2% animals had + positivity, 20.8% animals had ++ positivity, 51.4% animals had +++ positivity and 22.5% animals had ++++ positivity. Amongst environmental determinants, breed, season, sex and age at vaccination proved to be non-significant sources of variation (P > 0.05). MHC genotypes with DRB1 gene and DQA2 genes also revealed non-significant association with ET vaccine response; however, a trend of decreasing PI values with increasing ranks was observed. Study revealed strong response of epsilon toxin along with complexity of the ET vaccine response as phenotype to be explained by genetic and non-genetic factors. The importance of better management practices and vaccination is suggested for preventive measures.

Concurrent Infection of Pestes Des Petits Ruminants and Enterotoxaemia in an Organized Goat Farm

“…Vaccination history of these animals was not clearly portrayed and warrants regular administration to prevent this important disease of small ruminants (Gowane et al, 2017). Thus this report summarizes the incidence of enterotoxaemia in stall-fed goats and Priyadharshini et al kids after a primary PPR viral infection.…”

Association between the Ovine MHC-DRB1 Gene and its Resistance to Gastrointestinal Parasites in Deccani Sheep Raised in Hot Semi-arid Ecosystem of India

“…The DNA was quantified using UV spectrophotometer. Sequence specific primers reported by Gowane et al (2017a) and Gowane et al (2017b) were utilized to amplify the region of interest. The polymerase chain reaction (PCR) was carried out in a total volume 50 μl solution containing 1 μl template DNA, 10X buffer with Mgcl 2 5 μl, 2.5 Mm dNTPs (100 µM each) 1.0 μl, forward primer (20 pmol /μl) 1.0 μl, reverse primer (20 pmol/μl) 1.0 μl and Taq DNA polymerase 1.0 μl.…”