Genetic, antigenic, and pathogenic characteristics of avian infectious bronchitis viruses genotypically related to 793/B in China

Han, Zongxi; Zhao, Wenran; Chen, Yuqiu; Xu, Qianqian; Sun, Jiazhi; Zhang, Tingting; Zhao, Yan; Liang, Sophie Hsin-Yi; Gao, Mengying; Wang, Qiuling; Kong, Xiangang; Liu, Shengwang

doi:10.1016/j.vetmic.2017.01.039

Cited by 17 publications

(15 citation statements)

References 41 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is likely the case for the emergence of isolate γCoV/ck/China/I0114/14 in this study and also cases found in other countries such as in Italy and Spain (Moreno et al, 2017). In China, only limited numbers of 793/B type viruses have been detected and isolated in chicken flocks (Chen et al, 2015;Han et al, 2017). Furthermore, both the S1 gene and the complete genomic sequence analysis showed that most of the detected 793/B field strains were of the vaccines, or vaccine-derived strains .…”

Section: Discussionsupporting

confidence: 49%

Genome characterization, antigenicity and pathogenicity of a novel infectious bronchitis virus type isolated from south China

Jiang

Zhao

Han

et al. 2017

Infection, Genetics and Evolution

Self Cite

View full text Add to dashboard Cite

In 2014, three infectious bronchitis virus (IBV) strains, designated as γCoV/ck/China/I0111/14, γCoV/ck/China/I0114/14 and γCoV/ck/China/I0118/14, were isolated and identified from chickens suspected to be infected with IBV in Guangxi province, China. Based upon data arising from S1 sequence and phylogenetic analyses, the three IBV isolates were genetically different from other known IBV types, which represented a novel genotype (GI-29). Virus cross-neutralization tests, using γCoV/ck/China/I0111/14 as a representative, showed that genotype GI-29 was antigenically different from all other known IBV types, thus representing a novel serotype. Complete genomic analysis showed that GI-29 type viruses were closely related to and might originate from a GX-YL5-like virus by accumulation of substitutions in multiple genes. These GI-29 viral genomes are still evolving and diverging, particularly in the 3' region, although we cannot rule out the possibility of recombination events occurring. For isolate γCoV/ck/China/I0114/14, we found that recombination events had occurred between nsps 2 and 3 in gene 1 which led to the introduction of a 4/91 gene fragment into the γCoV/ck/China/I0114/14 viral genome. In addition, we found that the GI-29 type γCoV/ck/China/I0111/14 isolate was a nephropathogenic strain and high pathogenic to 1-day-old specific pathogen-free (SPF) chickens although cystic oviducts were not observed in the surviving layer chickens challenged with γCoV/ck/China/I0111/14 isolate.

show abstract

Section: Discussionsupporting

confidence: 49%

Genome characterization, antigenicity and pathogenicity of a novel infectious bronchitis virus type isolated from south China

Jiang

Zhao

Han

et al. 2017

Infection, Genetics and Evolution

Self Cite

View full text Add to dashboard Cite

show abstract

“…It has been reported that the side effects of the 4/91 vaccine might provide the basis for recombination with other strains. This phenomenon has been shown by several studies which have traced the emergence of many novel IBV strains to recombination events between the 4/91 vaccine and field strains (Feng et al, 2018;Han et al, 2017;Jiang et al, 2017Jiang et al, , 2018Liu et al, 2013;Mo et al, 2013;Ovchinnikova et al, 2011;Zhang et al, 2015Zhang et al, , 2016Zhou et al, 2017) in cases where the 4/91 vaccine strain could not provide effective protection against the field strains , leading to coinfection of the vaccine and field strains in the same chickens and ultimately resulting in recombination. In this study, we performed complete genome sequence analysis of strains I0718/17, I0722/17, I0724/17, and I0737/17 and confirmed the occurrence of recombination events at the 5′ ends of the S1 gene between a 4/91-like virus (GI-13 lineage) and a YX10-like virus (GI-19) (Xu et al, 2018), which contributed to the emergence of the IBV strains.…”

Section: Discussionmentioning

confidence: 87%

“…More recently, phylogenetic studies have reported that-in addition to the well-established lineages/genotypes such as GI-1, GI-7, GI-13, GI-19, GI-22, and GVI-1-a number of novel IBV variants have been isolated in China (Chen et al, 2015Han et al, 2017;Xu et al, 2016Xu et al, , 2018Zhao et al, 2017). These variants have been proved to be distinct from other reference strains on the basis of genetic characteristics and phylogenetic differences in the S1 gene (Xu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Genetic and biological characteristics of four novel recombinant avian infectious bronchitis viruses isolated in China

Ren

Sheng

et al. 2019

Virus Research

Self Cite

View full text Add to dashboard Cite

A B S T R A C TInfectious bronchitis viruses (IBVs) of GI-13 (793/B) and GI-19 (QX/LX4) lineages have been frequently detected in China in recent years. Naturally recombinant IBVs originating from the GI-13 and GI-19 lineages have also been isolated from chicken flocks with respiratory and renal problems in China. Thorough genetic and biological investigations of these recombinant viruses have led to speculation regarding their origin, evolution, and control. In order to confirm the previous results and further extend our understanding about the characteristics of the four recombinant IBV strains we had previously identified (I0718/17, I0722/17, I0724/17, and I0737/17), we conducted phylogenetic analysis by comparing their complete S1 gene sequences with those of 71 reference strains of different genotypes and lineages. We identified a close relationship between the S1 sequences of the four strains and those of GI-13 strains. The results of complete genome sequence analysis confirmed the previously identified recombination events in the four IBV strains and revealed additional recombination events in different genomic regions of strains I0718/17 and I0724/17, suggesting that the two strains originated from multiple recombination events between 4/91-like and YX10-like viruses. We comparatively evaluated the antigenicity, pathogenicity, and affinity of the four recombinant viruses and their deduced parental strains in the trachea and kidneys. Some of the strains showed comparable antigenic relatedness, pathogenicity, and affinity for the trachea and kidneys among each other and with their parental viruses; however, some of them showed varying biological characteristics. Point mutations observed in the receptor-binding domain and hypervariable region of the S1 subunit of the spike protein likely have an effect on these differences in biological characteristics, although the influence of other factors-such as host innate-immune responses and changes in genomic regions beyond the S1 protein-might also be responsible for such changes.

show abstract

“…High frequencies of recombination between vaccines (such as the 4/91 strain) and field strains have been reported frequently in China and other parts of the world (Cavanagh et al, 1992a,b;Jackwood et al, 2012;Liu et al, 2013;Han et al, 2017;Jiang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…It has been suggested that the emergent IBV strains in China may have different origins. It is considered that some of the IBV types circulating in China, such as Mass and 793/B, are the most widely distributed types worldwide and they may have infected Chinese chickens from an exogenous source, probably due to the use of live vaccines (Chen et al, 2015;Han et al, 2017). By contrast, some IBV types, such as LX4 and ck/CH/LDL/97I (Q1-like), are believed to have originated in China and spread to other regions of the world (Valastro et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the complete genome, antigenicity, pathogenicity, tissue tropism, and shedding of a recombinant avian infectious bronchitis virus with a ck/CH/LJL/140901-like backbone and an S2 fragment from a 4/91-like virus

et al. 2018

Self Cite

View full text Add to dashboard Cite

In this study, we isolated an infectious bronchitis virus, designated I1101/16, from broiler breeders in China. Analysis of the S1 gene showed that isolate I1101/16 was genetically close to strain ck/CH/LJL/140901, which belongs to the TW I genotype (also known as lineage GI-7 based on the recent IBV classification), however the S2 gene showed genetic diversity comparing to that of S1 gene. Comparison of the genomic sequences showed that the genome of isolate I1101/16 was similar to that of strain ck/CH/LJL/140901 from the 5' end of the genome to the 5' end of the S2 gene and from the 5' end of the 3a gene to the end of the genome, whereas the remaining parts of the genome sequences were more closely related to those of strain 4/91 than those of ck/CH/LJL/140901, thereby suggesting that recombination might have occurred during the origin of the virus. SimPlot and Bootscan analysis of the complete genomic sequence confirmed this hypothesis, where it showed that isolate I1101/16 arose through recombination events between ck/CH/LJL/140901- and 4/91-like viruses. Isolate I1101/16 and strain ck/CH/LJL/140901 shared identical amino acids in almost all five of their B cell epitopes, but the two viruses had a serotype relatedness value of 65, which is well below 80, i.e., the lower cutoff value for viruses of the same serotype. In addition, pathogenicity tests demonstrated that isolate I1101/16 was more pathogenic to 1-day-old specific-pathogen-free chickens than strain ck/CH/LJL/140901, according to analysis of the clinical signs, whereas strain ck/CH/LJL/140901 exhibited prolonged replication and shedding after challenge compared with isolate I1101/16. This study did not provide evidence that recombination can directly alter the antigenicity, virulence, replication, shedding, and tissue tropism of a virus, but it did show that recombination events are likely to be major determinants of viral evolution.

show abstract

Genetic, antigenic, and pathogenic characteristics of avian infectious bronchitis viruses genotypically related to 793/B in China

Cited by 17 publications

References 41 publications

Genome characterization, antigenicity and pathogenicity of a novel infectious bronchitis virus type isolated from south China

Genome characterization, antigenicity and pathogenicity of a novel infectious bronchitis virus type isolated from south China

Genetic and biological characteristics of four novel recombinant avian infectious bronchitis viruses isolated in China

Characterization of the complete genome, antigenicity, pathogenicity, tissue tropism, and shedding of a recombinant avian infectious bronchitis virus with a ck/CH/LJL/140901-like backbone and an S2 fragment from a 4/91-like virus

Contact Info

Product

Resources

About