Genetic and toxigenic diversity of Bacillus cereus group isolated from powdered foods

Sánchez-Chica, Jennifer; Correa, Margarita M.; Aceves-Díez, Ángel E.; Castañeda-Sandoval, Laura M.

doi:10.1007/s13197-020-04700-2

Cited by 9 publications

(15 citation statements)

References 32 publications

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“…Worldwide, incidences of B. cereus s.l. are variable; the values reported are either higher or lower than the ones obtained in this study; however, as long as the food raw-materials and flours are handcrafted and slightly processed, they are susceptible to contamination by B. cereus spores and vegetative cells, constituting great risk for human health [ 12 , 13 ]. Studies performed in countries such as Korea found B. cereus in 40% of raw rice samples [ 23 ]; in Iran, 42% of infant formula analyzed carried B. cereus [ 24 ] and in New Zealand, B. cereus incidences in dehydrated potato ranged from 10% to 40% [ 25 ].…”

Section: Discussionmentioning

confidence: 90%

“…was detected in 43 of 75 (57%) cassava starch samples. This is considered a relatively high incidence if compared to values obtained during the analysis of other food matrices in Colombia such as powdered foods that showed incidences from 10% to 13% [ 13 ]. Possibly, the high presence of B. cereus s.l.…”

Section: Discussionmentioning

confidence: 99%

“…The genomic heterogeneity of B. cereus s.l. isolated from cassava starch was evaluated by repetitive element palindromic-polymerase chain reaction (rep-PCR) using (GTG) 5 primer, as described previously [13]. Briefly, the 20 µL reaction contained 0.6 µM of (GTG) 5 primer, 200 µM of dNTPs mix, 3.5 mM of MgCl 2 , 1.6 U of Taq DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA ), 2 µL of 10X PCR buffer and 50 ng of DNA template.…”

Section: Bacillus Cereus Sl (Gtg) 5 -Pcr Fingerprintingmentioning

confidence: 99%

“…Various works around the world have reported a high genomic diversity on B. cereus s.l. from foods such as ready-to-eat [ 11 , 12 ] and powdered products [ 13 ], milk [ 6 , 14 ] and others [ 15 ], as well as a broad distribution of toxin genes in B. cereus s.l. isolated from foods.…”

Section: Introductionmentioning

confidence: 99%

“…In Colombia, B. cereus s.l. isolated from powdered foods [ 13 ] and ready-to-eat foods [ 12 ] contained the genes for enterotoxins production. Also, a previous study reported the detection of B. cereus s.l.…”

Section: Introductionmentioning

confidence: 99%

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Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch

Sánchez-Chica

Correa

Aceves-Díez³

et al. 2021

Toxins

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Bacillus cereus is a human pathogenic bacterium found in foods with the potential to cause emesis and diarrhea. This study estimated the presence, toxigenic and genomic diversity of B. cereus s.l. obtained from cassava starch samples collected in bakeries and powdered food companies in Medellín (Colombia). Bacillus cereuss.l. was found in 43 of 75 (57%) cassava starch samples and 98 isolates were obtained. The nheABC, hblCDAB, cytK2, entFM and cesB toxin genes were detected by multiplex PCR and the most frequent operon was nheABC, whereas cesB gene was not found. Twelve toxigenic profiles were determined by the detection of toxin genes, and the most frequent profiles harbored all enterotoxin genes. A broad genomic diversity was detected according to GTG5-PCR fingerprinting results with 76 B. cereus s.l. grouped in sixteen clusters and the 22 isolates clustering separately. No relationship was observed between genomic background and toxigenic profiles. In general, the results showed a high genomic and enterotoxigenic diversity in B. cereus s.l. found in cassava starch. These results should incentive future studies to understand the distribution of B. cereus s.l. isolated on raw materials in comparison with finished products.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Bacillus Cereus Sl (Gtg) 5 -Pcr Fingerprintingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch

Sánchez-Chica

Correa

Aceves-Díez³

et al. 2021

Toxins

Self Cite

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Prevalence of Bacillus cereus in dairy powders focusing on its toxigenic genes and antimicrobial resistance

2022

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Bacillus cereus is a common environmental foodborne microorganism that is mainly found to harbor toxigenic genes with multiple antibiotic resistances and is linked to threatening the safety of dried milk in concern to powdered infant milk formula. In the current investigation, the mean value of B. cereus in 140 samples of powdered milk was 0.57 × 102 ± 0.182 × 102, 0.15 × 102 ± 0.027 × 102, 0.21 × 102 ± 0.035 × 102, and 0.32 × 102 ± 0.072 × 102 CFU/g in a percentage of 64.0 samples of whole milk powder, 43.3 of skim milk powder, 26.7 of powdered infant milk formula and 36.7 milk–cereal-based infant formula, respectively. The results revealed that B. cereus isolates were found to harbor toxigenic genes in the following percentages: 77.8, 2.0, 72.7, 16.2, and 67.7 for nhe, hbl, cytK, ces, and bceT, respectively. Despite all evaluated B. cereus strains were originated from dairy powders, they showed a significant difference (P < 0.05) in their harbored toxigenic cytK gene between whole and skim milk powders with powdered infant formula and milk–cereal-based infant formula, as well as between powdered infant formula and milk–cereal-based infant formula. All isolated B. cereus strains were resistant to cefoxitin, colistin sulfate, neomycin, trimethoprim–sulfamethoxazole, oxacillin, and penicillin. Based on the antimicrobial resistance of B. cereus strains to cephalothin, chloramphenicol, nalidixic acid, and tetracycline, there was a significant difference (P < 0.05) between powdered infant milk formula and whole milk powder strains. This survey is one of few studies proceeded in Egypt to determine the prevalence of toxigenic B. cereus strains in milk–cereal-based infant formula and powdered infant formula as well as skim milk powder.

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Microbiological hazards in infant and toddler food in China: A comprehensive study between 2004 and 2022

Teng,

Huang,

Zhou

et al. 2024

Food Research International

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Genetic and toxigenic diversity of Bacillus cereus group isolated from powdered foods

Cited by 9 publications

References 32 publications

Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch

Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch

Prevalence of Bacillus cereus in dairy powders focusing on its toxigenic genes and antimicrobial resistance

Microbiological hazards in infant and toddler food in China: A comprehensive study between 2004 and 2022

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