Genetic and radiation-reduced somatic cell hybrid sublocalization of the human GSTP1 gene

Abstract

A number of related enzymes like glutathione S-transferases (GSTs) and fatty acid ethyl ester synthases (FAEESs) have been implicated in detoxification and drug resistance. The anionic class of GSTs, π, and closely related FAEES-III exhibit tissue-specific and developmentally regulated expression, and the former has been shown to be overex-pressed or amplified in a variety of tumors. The GSTPl gene has previously been cloned and cytogenetically localized to human 11q13 by in situ hybridization. Using a series of previously described radiation-reduced somatic cell hybrids, we have sublocahzed GSTP1 to 11q13. We isolated a genomic clone containing the entire GSTPl gene and sequenced it. Analysis of the 5’ region revealed 23 (TAAAA) tandem repeats interrupted by a single TA and TAA insertion. This repeat number differs among individuals. Eleven alleles in a mostly Caucasian sample were observed. This repeat has a polymorphism information content of 0.74. Linkage analysis of the Venezuelan reference pedigree places GSTP1 5 cM distal to PYGM and 4 cM proximal to FGF3 thereby providing a genetic marker half-way between these two loci. The sublocalization and genetic characterization of GSTPl facilitates linkage analysis of several disease genes mapped to this chromosome band as well as the correlation of genetic and physical markers in the region.

Human Uteroglobin Gene: Structure, Subchromosomal Localization, and Polymorphism

“…3). This result is also supported by our recent results with radiation-reduced somatic cell hybrids (Smith et al, 1995a (Chock et al, 1994). Furthermore, cDNAs encoding a high-affinity receptor for PLA2-I have recently been cloned and characterized (Ishizaki era/., 1994;Lambeau et al, 1994;Ancian étal, 1995 (a marker distal to PYGM) and D11S451 (Sanford et al, 1993), with the highest location scores being between D11S480 and FceRI-0.…”

“…3), where we assign the locus of hUG gene. We used a series of somatic cell and radiation-reduced somatic cell hybrids described previously (Smith et al, 1995a) to map the hUG gene with respect to other genes in 1 lq 12.1-13.2. The UG hybridization pattern (Fig.…”

Promiscuous translocations into immunoglobulin heavy chain switch regions in multiple myeloma

“…PCR primers were designed to amplify the sequence from the unknown end (i.e., the end that did not contain sequences from the selected switch region) of the illegitimate switch region fragment clone. PCR analysis was performed on the following mapping panels: (i) NIGMS Mapping Panel 2 (Coriell Cell Repository, Camden, NJ), each somatic cell hybrid contains a single human chromosome; (ii) BIOS mapping panel, each hybrid contains several human chromosomes; (iii) GENEBRIDGE4 radiation hybrid panel (Research Genetics, Huntsville, AL) (27); and (iv) an 11q13-specific panel of radiation hybrids (28,29). Sublocalization was done by fluorescence in situ hybridization, sequence identity to known chromosomal regions, or mapping to cosmid or yeast artificial chromosome clones containing genomic sequences (30)(31)(32).…”

Mapping eight new polymorphisms in 11q13 in the vicinity of multiple endocrine neoplasia type 1: identification of a new distal recombinant

“…The SSR has a PIC value of 0.73 and is located within the 5" promoter region of the glutathione-S- Fig.2 Order of markers by radiation-reduced somatic cell hybrid analysis. Loci shown above the line were used to characterize a series of radiation-reduced somatic cell hybrids into segregation groups (Gerhard et al 1992, Smith et al 1995. Seven new RFLPidentifying bacteriophage clones and one SSR polymorphisms (shown below the line) were characterized into four of these segregation groups along I I q 13 transferase rt (GSTP1) gene.…”

“…Seven new RFLPidentifying bacteriophage clones and one SSR polymorphisms (shown below the line) were characterized into four of these segregation groups along I I q 13 transferase rt (GSTP1) gene. Genetic analysis places the GSTP1 locus between PYGM and FGF3 (Smith et al 1995; Table 3). …”