Genetic and physical studies of Streptococcus faecalis hemolysin plasmids

Borderon, E

doi:10.1016/0378-1097(82)90041-6

Cited by 7 publications

(10 citation statements)

References 9 publications

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“…We reported recently that D398 harbours a haemolysin-bacteriocin plasmid, pIP1441, which transfers at a high frequency (3 x 10-1 transconjugants per donor) into plasmid-free, non-haemolytic E. faecalis (Bieth et al, 1985). Conjugative plasmids encoding haemolysin-bacteriocin are common in /3-haemolytic E. faecalis (Borderon et al, 1982).…”

Section: Results a N D Discussionmentioning

confidence: 99%

Use of Penicillin-binding Proteins for the Identification of Enterococci

et al. 1986

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The results of 20 physiological and fermentation tests and examination of the penicillin-binding proteins (PBPs) of 85 enterococcal strains demonstrated that the genus Enterococcus could be divided into at least nine distinct species : E. faecalis, E. faecium, E. durans, E. hirae, E. auium, E. gallinarum, E. casselijlavus, E. malodoratus and E. mundtii. Each species had a specific pattern of at least five PBPs, with molecular masses in the range of about 40-1 30 kDa. The pattern of PBPs may be useful for identification purposes, since some strains with unusual fermentation characteristics were assigned to species by this technique.

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Section: Results a N D Discussionmentioning

confidence: 99%

Use of Penicillin-binding Proteins for the Identification of Enterococci

et al. 1986

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“…pAD1 per se proved to be a member of a widely disseminated family of conjugative, cytolysin-encoding plasmids, especially those associated with opportunistic enterococcal infections in humans. 42,[61][62][63][64] Interestingly, pAD1 was essentially indistinguishable from the pAMγ1 plasmid of strain DS5 discussed earlier in reference 42. The cytolysin activity of pAD1 was soon found to enhance pathogenicity in animal infection models, and S. faecalis strains associated with human parenteral infections were found more likely to be hemolytic than fecal isolates.…”

Section: S Faecalis Ds16 and A Wealth Of Interesting Genetic Elementsmentioning

confidence: 99%

Tales of conjugation and sex pheromones

Clewell¹

2011

Mobile Genetic Elements

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REVIEW REVIEW use of a technique that had recently been reported by Godson and Sinsheimer 6 that utilized a detergent mixture of Brij 58 (a neutral detergent) and deoxycholate for lysis of phageinfected E. coli. During a relatively low-speed centrifugation step, the vast majority of chromosomal DNA pelleted leaving the supernatant greatly enriched in plasmid DNA. Sucrose gradient analyses of these "cleared lysates" revealed a ColE1-protein complex sedimenting slightly faster (24S) than the 23S protein-free supercoiled DNA. Surprisingly, when the 24S complex was exposed to conditions expected to affect protein structure such as proteases, strong ionic detergents or heat, the 24S complex converted to a 17S relaxed (open circular) configuration. The relaxation event involved the introduction of a strand-specific nick in the supercoiled plasmid; we therefore called the 24S entity a "relaxation complex". 7,8 The percentage of plasmid DNA that was present in the complexed state varied from less than 30%, when glucose was in the growth medium, to more than 80% when glucose was absent.

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“…The hemolysin-bacteriocin (Hly-Bcn) plasmids piP964 and pAD1 of Enterococcus faecalis are structurally related conjugative plasmids which make use of sex pheromones to induce cell-to-cell contact and transfer in broth at relative high frequencies (10-1-10-3 per donor) [1][2][3][4]. In this system, the donor senses the small peptide sexpheromone cAD1 excreted by the plasmid-free recipient and responds by producing a membrane-bound protein, called the aggregation substance, which induces the formation of mating aggregates [3].…”

Section: Introductionmentioning

confidence: 99%

Heterogeneric conjugal transfer of the pheromone-responsive plasmid pIP964 (IncHlyI) ofEnterococcus faecalisin the apparent absence of pheromone induction

Poyart

Trieu-Cuot

1994

FEMS Microbiology Letters

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Erythromycin-resistant derivatives of the pheromone-responsive plasmid pIP964 from Enterococcus faecalis were constructed to study its host range. This was done by inserting the integrative vector pAT112 and the related replicon pTCR1 harboring oriR of the broad host range plasmid pAM beta 1 into the hemolysin-bacteriocin operon of pIP964, to give pTCR2 and pTCR3, respectively. Plasmid pTCR2 was transferred by filter matings from E. faecalis to Enterococcus faecium and Listeria monocytogenes at frequencies of 2 x 10(-7) and 5 x 10(-7) per donor, respectively, in the apparent absence of pheromone induction and cellular aggregation. In these hosts, pTCR2 remained intact as a self-replicating element and maintained its transfer capabilities. Plasmid pTCR3, but not pTCR2, was transferred at similar frequencies from E. faecalis to Lactococcus lactis and Streptococcus agalactiae. Thus, the transfer system of pIP964 possesses a broader host-range than its replication system.

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Genetic and physical studies of Streptococcus faecalis hemolysin plasmids

Cited by 7 publications

References 9 publications

Use of Penicillin-binding Proteins for the Identification of Enterococci

Use of Penicillin-binding Proteins for the Identification of Enterococci

Tales of conjugation and sex pheromones

Heterogeneric conjugal transfer of the pheromone-responsive plasmid pIP964 (IncHlyI) ofEnterococcus faecalisin the apparent absence of pheromone induction

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