Genetic and phylogenetic analyses of capsid protein gene in feline calicivirus isolates from Rio Grande do Sul in southern Brazil

Henzel, Andréia; Silva, M. Sá e; Luo, Shuhong; Lovato, Luciane Teresinha; Weiblen, Rudi

doi:10.1016/j.virusres.2011.12.008

Cited by 17 publications

(22 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, D region is more conserved and ConE showed little variability with AA changes in five residues. The isolate RJ029/2013 and other two isolates of a previous Brazilian study (sv1425/1993, sv160/2002) 21 had a close relationship with vaccine strain. They revealed AA substitution only at position 397, 398, 480 and 524.…”

Section: Resultsmentioning

confidence: 64%

“…However, most of the phylogenetical analysis typically results in a “star-like” phylogeny with little statistical support for grouping FCV strains in sub-species clusters either divide them into spatial, temporal or clinical grounds. 15 , 21 , 37 , 38 , 39 The highest evolutionary rates of the virus lead to a saturation of evolutionary signals rapidly leading to a loss of phylogenetic resolution over relatively short time periods. 38 …”

Section: Discussionmentioning

confidence: 99%

“…Among these prototypes, 13 Brazilian isolates from a molecular study performed in Southern Brazil were included. 21 These were the only Brazilian FCV capsid sequences available so far in GenBank. Prototypes were aligned using the CLUSTAL W method into BioEdit ® program 22 and analyzed using DAMBE software 23 for the exclusion of equal sequences.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil

Pereira

Baumworcel

Fioretti

et al. 2018

Brazilian Journal of Microbiology

View full text Add to dashboard Cite

The aim of this study was to perform the molecular characterization of conserved and variable regions of feline calicivirus capsid genome in order to investigate the molecular diversity of variants in Brazilian cat population. Twenty-six conjunctival samples from cats living in five public short-term animal shelters and three multicat life-long households were analyzed. Fifteen cats had conjunctivitis, three had oral ulceration, eight had respiratory signs (cough, sneeze and nasal discharge) and nine were asymptomatic. Feline calicivirus were isolated in CRFK cells and characterized by reverse transcription PCR target to both conserved and variable regions of open reading frame 2. The amplicons obtained were sequenced. A phylogenetic analysis along with most of the prototypes available in GenBank database and an amino acid analysis were performed. Phylogenetic analysis based on both conserved and variable region revealed two clusters with an aLTR value of 1.00 and 0.98 respectively and the variants from this study belong to feline calicivirus genogroup I. No association between geographical distribution and/or clinical signs and clustering in phylogenetic tree was observed. The variants circulating in public short-term animal shelter demonstrated a high variability because of the relatively rapid turnover of carrier cats constantly introduced of multiple viruses into this location over time.

show abstract

Section: Resultsmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil

Pereira

Baumworcel

Fioretti

et al. 2018

Brazilian Journal of Microbiology

View full text Add to dashboard Cite

show abstract

“…Serological studies on domestic and wild cats have been performed in RS and in the West-Central region of Brazil (RUTHNER-BATISTA et al, 2005;JOHANN et al, 2009;HENZEL et al, 2013). The molecular characterization of FCV isolates has also been reported (HENZEL et al, 2012a). However, the current distribution and prevalence of these viruses is largely unknown in Brazil.…”

Section: Introductionmentioning

confidence: 99%

Epidemiological status of felid herpesvirus type-1 and feline calicivirus infections in Brazil

2015

View full text Add to dashboard Cite

show abstract

“…The capsid protein of FCV is an important target for the immune response and molecular studies have mapped antigenic epitopes located in the HVR_E (Radford et al, 1999;Geissler et al, 2002). Since these amino acid differences occurred in a putatively neutralising region of HVR_E, the underlying mutations might represent a mechanism for FCV immune evasion and possibly also differences in viral pathogenicity (Henzel et al, 2012). The results of our phylogenetic analyses showed that clustering of reference viruses occurs independent of clinical signs; in particular VS-FCV associated with haemorrhagic disease are spread over different lineages of the phylogenetic tree.…”

Section: Discussionmentioning

confidence: 99%

Virulent feline calicivirus disease in a shelter in italy: A case description

Battilani

Carelle

et al. 2013

Research in Veterinary Science

View full text Add to dashboard Cite

Feline calicivirus (FCV) is a common pathogen of cats that is particularly widespread in multi-cat environments such as shelters and catteries. FCV infections are usually associated with acute, mild and self-limiting upper respiratory tract disease characterized by oral vesicles/ulcers. Recently, virulent systemic disease (VSD) associated with FCV infection has been reported in the USA and Europe. This paper describes a case of VSD affecting one adult, FIV infected cat ("Oscar") living in a shelter located in Northern Italy; the clinical, post-mortem and laboratory findings indicate that this is the first case of suspected FCV-VSD in this country. Similar to a previous report (Meyer et al., 2011), the disease affected only one cat, while others remained asymptomatic, despite their direct contact with "Oscar". Phylogenetic analysis identified unique features in the "Oscar" FCV isolate. The FIV infection of the patient might have favoured the generation of the virulent FCV strains in this cat.

show abstract

Genetic and phylogenetic analyses of capsid protein gene in feline calicivirus isolates from Rio Grande do Sul in southern Brazil

Cited by 17 publications

References 29 publications

Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil

Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil

Epidemiological status of felid herpesvirus type-1 and feline calicivirus infections in Brazil

Virulent feline calicivirus disease in a shelter in italy: A case description

Contact Info

Product

Resources

About