Genetic and Phenotypic Identification of Vancomycin-Resistant Staphylococcus aureus Isolates from Retail Poultry Carcasses in Omu-Aran, North-Central Nigeria

Abstract

Staphylococcus aureus is a well-known agent of zoonotic infections. Livestock-associated methicillin-resistant S. aureus (LA-MRSA) had been receiving public health attention for over a decade. Recently, the genomes of some MRSA strains evolved further by enabling acquisition of vanA gene from enterococcus which drives the emergence of vancomycin-resistant S. aureus (VRSA), thus signaling a higher threat to antimicrobial chemotherapy and diagnostic microbiology. This study was designed to examine slaughtered chicken carcasses in Omu-Aran, North-Central Nigeria for the presence of VRSA using vancomycin agar screen (VAS) as recommended by the Clinical and Laboratories Standards Institute (CLSI). To provide independent witness to further support the evidences from VAS, a 235 bp marker for vanA gene was simultaneously detected by Original Research Article

Molecular identification of Methicillin-resistant staphylococcus aureus (MRSA) and Vancomycin- resistant staphylococcus aureus (VRSA) strains isolated from milk and milk products.

“…These findings agree with those of Kapllan et Tiwari and Sen (2006), who reported that vancomycin resistance isolates could be detected phenotypically while not carrying vanA and vanB genes. Several reports showed various levels of correlation between gene detection and agar-based identification of staphylococcal vancomycin-resistant phenotype (Okolie et al, 2015). Failure of PCR to detect the genes encoding vancomycin resistance could be multifactorial, ranging from poor primer to the absence of the target gene (or sequence) within the genome.…”

Molecular detection of antibiotic resistance and virulence genes in staphylococcus species isolated from human and poultry

“…6). Martins et al (2013) detected vanA gene in 3 samples out of 15 of chilled industrialized uncooked chicken parts and Okolie et al(2015) found vanA in 22 isolates out of 155 from chicken carcasses. On other hand, Saadat et al (2014) detected van A in 34% of clinical isolates in hospital, while Khudaier(2018) detected van A in 4 human isolates out of 163 (2.5%).…”