Genetic and Phenotypic Diversity of the Sunflower Collection of the Pustovoit All-Russia Research Institute of Oil Crops (VNIIMK)

Goryunova, S. V.; Goryunov, Denis V.; Chernova, Alina; Martynova, Elena; Dmitriev, A.E.; Boldyrev, S. V.; Ayupova, Asiya F.; Mazin, Pavel; Gurchenko, E.A.; Pavlova, Anna; Petrova, Daria; Chebanova, Yuliya; Горлова, Л.А.; Garkusha, Sergey; Mukhina, Zhanna; Savenko, Elena; Demurin, Ya.N.

doi:10.1515/helia-2018-0021

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…Few cultivated species have been domesticated outside their region of origin. For example, sunflower from South America was developed into oilseed sunflower in Russia [ 55 , 56 , 57 ]; grapefruit, a hybrid of Citrus sinensis from Southeast Asia and Citrus maxima from Indonesia, was domesticated in Barbados in the 1820s [ 58 ]; and Chinese gooseberry ( Actinidia spp.) from East Asia was domesticated and commercialised in New Zealand as kiwifruit [ 59 , 60 ].…”

Section: Origin Of Cultivated Species and Geographic Distribution Of ...mentioning

confidence: 99%

Management and Utilization of Plant Genetic Resources for a Sustainable Agriculture

Pathirana

Carimi

2022

Plants

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Despite the dramatic increase in food production thanks to the Green Revolution, hunger is increasing among human populations around the world, affecting one in nine people. The negative environmental and social consequences of industrial monocrop agriculture is becoming evident, particularly in the contexts of greenhouse gas emissions and the increased frequency and impact of zoonotic disease emergence, including the ongoing COVID-19 pandemic. Human activity has altered 70–75% of the ice-free Earth’s surface, squeezing nature and wildlife into a corner. To prevent, halt, and reverse the degradation of ecosystems worldwide, the UN has launched a Decade of Ecosystem Restoration. In this context, this review describes the origin and diversity of cultivated species, the impact of modern agriculture and other human activities on plant genetic resources, and approaches to conserve and use them to increase food diversity and production with specific examples of the use of crop wild relatives for breeding climate-resilient cultivars that require less chemical and mechanical input. The need to better coordinate in situ conservation efforts with increased funding has been highlighted. We emphasise the need to strengthen the genebank infrastructure, enabling the use of modern biotechnological tools to help in genotyping and characterising accessions plus advanced ex situ conservation methods, identifying gaps in collections, developing core collections, and linking data with international databases. Crop and variety diversification and minimising tillage and other field practices through the development and introduction of herbaceous perennial crops is proposed as an alternative regenerative food system for higher carbon sequestration, sustaining economic benefits for growers, whilst also providing social and environmental benefits.

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Section: Origin Of Cultivated Species and Geographic Distribution Of ...mentioning

confidence: 99%

Management and Utilization of Plant Genetic Resources for a Sustainable Agriculture

Pathirana

Carimi

2022

Plants

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“…Oleic acid content was estimated by extracting fatty acids (FAs) and measuring FA composition. Half-seeds were homogenized and then subjected to fatty acid (FA) extraction with hexane followed by subsequent esteri cation as previously described (Goryunova et al 2019). The measurement was performed by means of Gas chromatography with ame ionization detection (GC-FID) using Chromateck-Crystal 5000 GC chromatograph with the DAG-2 M automatic dispensor.…”

Section: Phenotyping Proceduresmentioning

confidence: 99%

QTL mapping of oleic acid content in modern VNIIMK sunflower (Helianthus annuus L. ) lines by using GBS-based SNP map

Gubaev

Boldyrev

Martynova

et al. 2022

Preprint

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Oleic acid is a monounsaturated fatty acid increasing oil oxidative stability. High content of oleic acid is thus a valuable trait in oilseed crops. Sunflower (Helianthus annuus L.) normally accumulates linoleic acid as a major fatty acid, but a mutant expressing a high oleic phenotype form was previously obtained by chemical mutagenesis and mapped on the sunflower genome. Several studies suggest the presence of additional genes involved in the control of the high content of oleic acid, with their expression possibly depending on the genetic background. To test this hypothesis, we performed a QTL mapping of the high oleic acid trait within two independent F2 crosses involving lines with contrasting oleic acid content from the Pustovoit All-Russia Research Institute of Oil Crops (VNIIMK) collection. We applied genotyping-by-sequencing (GBS) to construct single nucleotide polymorphism-based genetic maps and performed QTL mapping using quantitative and qualitative encoding for oleic acid content. Our results support the finding that the oleic acid content in the assessed crosses is controlled by one major effect locus. However, different dominant/recessive effects of the major locus were reported for both crosses. Additionally, a possible translocation between chromosome 7 and 14 was reported in one assessed cross. We defined a set of single nucleotide polymorphism markers for each cross which could be used for marker-assisted selection.

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“…Порядка 2000 SSR-маркеров были разработаны ранее на основе геномных (gSSR) и EST (EST-SSR) библиотек [4,5,6]. SNP-технологии бурно развиваются в последнее десятилетие [7,8,9]. Аллельной изменчивостью в локусах SSR и SNP управляют различные мутационные процессы, частота мутаций SNP на несколько порядков ниже, чем у SSR.…”

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Genetic diversity of the Russian sunflower breeding lines revealed by microsatellite loci analysis

Guchetl

Golovatskaya

Рамазанова

et al. 2023

Аграрная наука Евро-Северо-Востока

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The breeding of high-yielding sunflower varieties and hybrids requires the use of parent material with considerable genetic diversity. It can be identified using molecular genetic markers. The purpose of this study was the genotyping of sunflower lines bred by V. S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK), developed in different ecological areas of cultivation, and to evaluate their genetic diversity using microsatellite loci. For the studies conducted in 2020-2022, there were used 23 lines from the Central experimental base (СEB), 17 lines from L.A. Zhdanov Don experimental station (DES), and 10 lines from the Armavir experimental station (AES). DNA was isolated from seedlings by the CTAB method. Samples were genotyped using 12 SSR markers. PCR products were separated in 8 % denaturing polyacrylamide gel or by capillary electrophoresis in a genetic analyzer. The main indicators of genetic diversity for all collections did not differ significantly from each other, and were moderate and increased in the collections of VNIIMK: AES – DES – CEB -the joint collection. In total there have been identified 37 alleles, an average 3.083 alleles per locus. The effective number of alleles ranged from 1.094 to 3.290 with an average value of 2.154. The values of the polymorphic information content (PIC) index ranged from 0.084 to 0.651, with an average of 0.434. The values of observed and expected heterozygosity ranged from zero to 0.071, 0.030, on average, and from 0.086 to 0.696, 0.500, on average, respectively. The number of shared alleles between different collections was 23. Molecular variation analysis revealed that most of the total variance (91 %) was due to differences between lines within each collection and 3 % to differences between collections. According to the results of the cluster analysis, the maternal lines from the collections of the CEB, DES, and AES of VNIIMK were mostly grouped into a cluster or subcluster separate from the paternal ones. The obtained results indicate a moderate genetic diversity of the studied sunflower lines of the breeding of VNIIMK and the existence of small differences between the collections.

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Genetic and Phenotypic Diversity of the Sunflower Collection of the Pustovoit All-Russia Research Institute of Oil Crops (VNIIMK)

Cited by 4 publications

References 17 publications

Management and Utilization of Plant Genetic Resources for a Sustainable Agriculture

Management and Utilization of Plant Genetic Resources for a Sustainable Agriculture

QTL mapping of oleic acid content in modern VNIIMK sunflower (Helianthus annuus L. ) lines by using GBS-based SNP map

Genetic diversity of the Russian sunflower breeding lines revealed by microsatellite loci analysis

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