Genetic and Phenotypic Characterization of theblaCMYGene fromEscherichia coliandSalmonella entericaIsolated from Food-Producing Animals, Humans, the Environment, and Retail Meat

Heider, Luke C.; Hoet, Armando E.; Wittum, Thomas E.; Khaitsa, Margaret L.; Love, Brenda C.; Huston, Carla L.; Morley, Paul S.; Funk, Julie A.; Gebreyes, Wondwossen A.

doi:10.1089/fpd.2009.0294

Cited by 32 publications

(16 citation statements)

References 20 publications

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“…Differently from ESBL determinants, the bla CMY-2 gene was found in 62.3% (38/62) of SH strains ( Table 2). The CMY-2 is related to resistance with second generation cephalosporins and penicillins through the production of β-lactamase AmpC [41] and it has been reported as major determinant of CEF resistance in Enterobacteriaceae from poultry source [42][43][44]. No others plasmid-mediated ampC genes investigated in our study were found in SH strains, including bla FOX and bla CMY-1 .…”

Section: β-Lactam Resistance Genesmentioning

confidence: 59%

High occurrence of β-lactamase-producing Salmonella Heidelberg from poultry origin

et al. 2020

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Salmonella Heidelberg is commonly reported in foodborne outbreaks around the world, and chickens and poultry products are known as important source of these pathogen. Multidrugresistant S. Heidelberg strains are disseminated into poultry production chair, which can lead to severe clinical infections in humans and of difficult to treat. This study aimed at evaluating the β-lactam susceptibility and genotypic relatedness of Salmonella Heidelberg at Brazilian poultry production chain. Sixty-two S. Heidelberg strains from poultry production chain (poultry, poultry meat and poultry farm) were used. All strains were evaluated to antimicrobial susceptibility by diffusion disk test, as well as β-lactam resistance genes. Genotypic relatedness was assessed by Pulsed-Field Gel Eletrophoresis, using Xba1 restriction enzyme. Forty-one strains were characterized as multidrug-resistant according to phenotype characterization. The resistance susceptibility revealed 31 distinct profiles, with higher prevalence of streptomycin (61/62), nalidixic acid (50/62), tetracycline (43/62) and β-lactam drugs (37/62). bla CMY-2 was the more frequent β-lactamase gene found (38/62); other resistance genes found were bla CTX-M (2/62), bla SHV (3/62) and bla TEM-1 (38/62). No carbapenemase genes was found. The Pulsed-Field Gel Electrophoresis showed 58 different profiles. Strains with a larger number of antimicrobial resistance were grouped into ten major clusters apart from others. The spread of resistance by ampC continues to rise, thereby turning concern to public health, since the β-lactam antimicrobials are used as a therapeutic treatment in humans.

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Section: β-Lactam Resistance Genesmentioning

confidence: 59%

High occurrence of β-lactamase-producing Salmonella Heidelberg from poultry origin

et al. 2020

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“…7,42 Resistance to third-generation cephalosporins is mediated by the b-lactamase gene blaCMY-2. 43 Concern for the possible role that antimicrobial use in cattle could play in promoting antimicrobial resistance in people was heightened after a child acquired a ceftriaxone-resistant Salmonella infection. but also between pathogenic Salmonella spp.…”

Section: Discussionmentioning

confidence: 99%

“…and commensal Escherichia coli. 43 Concern for the possible role that antimicrobial use in cattle could play in promoting antimicrobial resistance in people was heightened after a child acquired a ceftriaxone-resistant Salmonella infection. 44 It has been suggested that increased resistance to ceftriaxone is related to food-borne transmission of the blaCMY-2 in S. enterica infecting humans.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility of Salmonella isolates recovered from calves with diarrhoea in Australia

Izzo

Mohler

House

2011

Australian Veterinary Journal

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OBJECTIVE To identify the common serotypes and antimicrobial resistance patterns of Salmonella spp. associated with diarrhoea in Australian dairy calves under the age of 6 weeks. DESIGN Cross-sectional study. METHODS Faecal samples were collected from outbreaks of diarrhoea in dairy and dairy beef calves less than 6 weeks old. Samples were screened for Salmonella using standard enrichment culture techniques. The antimicrobial susceptibility to 12 commonly used veterinary and human antimicrobials was assessed using the Kirby-Bauer disk diffusion method and the susceptibility profiles of dairy and dairy beef properties were compared using Fisher's exact test. RESULTS Salmonella ser. Dublin, S. ser. Typhimurium and S. ser. Bovismorbificans were the three most common salmonella serotypes isolated. The majority of properties had one serotype. Most of the Salmonella isolates were not resistant to any of the antimicrobials tested. No resistance was seen to amikacin and nalidixic acid, and only one isolate was resistant to ceftiofur or amoxicillin-clavulanic acid. The most common antimicrobial resistance was to streptomycin, ampicillin or combination sulfonamides. Multi-drug resistance was detected in S. ser. Anatum, S. ser. Bovismorbificans, S. ser. Muenster, S. ser. Newport and S. ser. Typhimurium. Isolates from dairy beef properties were more likely to be resistant to ampicillin, kanamycin, neomycin, sulfamethoxazole/trimethoprim and tetracycline (P < 0.05) and were more likely to exhibit multi-drug resistance. CONCLUSION The prevalence of antimicrobial resistance in Salmonella isolates from dairy calves in Australia is low compared with that reported overseas. From a human health perspective, resistance to antimicrobials used in the treatment of human salmonellosis was infrequent.

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“…Breakpoints recommended by the Clinical and Laboratory Standards Institute (CLSI) or used by the National Anti‐microbial Resistance Monitoring System (NARMS) were used to classify isolates as having reduced susceptibility (resistant or intermediate) or susceptible. For isolates exhibiting resistance to 3rd generation cephalosporins, PCR was used to test for the presence of the AmpC gene bla CMY using previously described methods (Heider et al., ).…”

Section: Methodsmentioning

confidence: 99%

Distribution and Diversity of Salmonella Strains in Shipments of Hatchling Poultry, United States, 2013

Habing

Kessler

Mollenkopf

et al. 2014

Zoonoses and Public Health

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Summary Multistate outbreaks of salmonellosis associated with live poultry contact have been occurring with increasing frequency. In 2013, multistate outbreaks of salmonellosis were traced back to exposure to live poultry, some of which were purchased at a national chain of farm stores (Farm store chain Y). This study was conducted at 36 stores of Farm store chain Y and was concurrent with the timing of exposure for the human outbreaks of salmonellosis in 2013. We used environmental swabs of arriving shipment boxes of hatchling poultry and shipment tracking information to examine the distribution, diversity and anti-microbial resistance of non-typhoidal Salmonella (NTS) across farm stores and hatcheries. Isolates recovered from shipment boxes underwent serotyping, anti-microbial resistance (AMR) testing and pulsed-field gel electrophoresis (PFGE). Postal service tracking codes from the shipment boxes were used to determine the hatchery of origin. The PFGE patterns were compared with the PFGE patterns of NTS causing outbreaks of salmonellosis in 2013. A total of 219 hatchling boxes from 36 stores in 13 states were swabbed between 15 March 2013 and 18 April 2013. NTS were recovered from 59 (27%) of 219 hatchling boxes. Recovery was not significantly associated with species of hatchlings, number of birds in the shipment box, or the presence of dead, injured or sick birds. Four of the 23 PFGE patterns and 23 of 50 isolates were indistinguishable from strains causing human outbreaks in 2013. For serotypes associated with human illnesses, PFGE patterns most frequently recovered from shipment boxes were also more frequent causes of human illness. Boxes positive for the same PFGE pattern most frequently originated from the same mail-order hatchery. Only one of 59 isolates was resistant to anti-microbials used to treat Salmonella infections in people. This study provides critical information to address recurrent human outbreaks of salmonellosis associated with mail-order hatchling poultry.

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Genetic and Phenotypic Characterization of thebla_CMYGene fromEscherichia coliandSalmonella entericaIsolated from Food-Producing Animals, Humans, the Environment, and Retail Meat

Cited by 32 publications

References 20 publications

High occurrence of β-lactamase-producing Salmonella Heidelberg from poultry origin

High occurrence of β-lactamase-producing Salmonella Heidelberg from poultry origin

Antimicrobial susceptibility of Salmonella isolates recovered from calves with diarrhoea in Australia

Distribution and Diversity of Salmonella Strains in Shipments of Hatchling Poultry, United States, 2013

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