Genetic and phenotypic analysis of Vibrio cholerae non-O1, non-O139 isolated from German and Austrian patients

Schirmeister, Falko; Dieckmann, Ralf; Bechlars, Silke; Bier, Nadja; Faruque, Shah M.; Strauch, Eckhard

doi:10.1007/s10096-013-2011-9

Cited by 62 publications

(58 citation statements)

References 42 publications

(80 reference statements)

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“…For example, Lasch et al (2014) (20) and Schirmeister et al (2013) reported the same for Vibrio cholerae isolates (21) and such studies may represent the tip of the difficult-to-publish iceberg. After examination of successful reports on MALDI-TOF MS typing, it becomes evident that for some bacterial taxa, the available data are not consistent.…”

Section: Typing By Maldi-tof Ms: What Can We Learn From Pfge?mentioning

confidence: 99%

Microbial Typing by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Do We Need Guidance for Data Interpretation?

et al. 2015

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eThe integration of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) in clinical microbiology has revolutionized species identification of bacteria, yeasts, and molds. However, beyond straightforward identification, the method has also been suggested to have the potential for subspecies-level or even type-level epidemiological analyses. This minireview explores MALDI-TOF MS-based typing, which has already been performed on many clinically relevant species. We discuss the limits of the method's resolution and we suggest interpretative criteria allowing valid comparison of strain-specific data. We conclude that guidelines for MALDI-TOF MS-based typing can be developed along the same lines as those used for the interpretation of data from pulsed-field gel electrophoresis (PFGE). Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently been integrated into the routine diagnostic workflow of many industrial, pharmaceutical, and medical microbiology laboratories. It has already been thoroughly evaluated for the identification of clinically relevant bacterial species, including anaerobes, Gram-positive rods and cocci, Enterobacteriaceae, and miscellaneous Gram-negative (including nonfermentative) rods, with adequate to excellent results (1, 2).Species identification, however, is only a first step in the diagnostic workflow. The ability to quickly and reliably distinguish or "type" related bacterial isolates is essential for bacterial transmission studies and larger-scale epidemiological surveillance projects. "Conventional" phenotyping methods, such as multiple-susceptibility testing, phage typing, serotyping, biochemical typing, and several others, have been important contributors to our understanding of the epidemiology of community-and health careassociated infections. However, these methods all have practical limitations which render them largely unsuitable for comprehensive bacterial population analyses as well as for scientifically less ambitious but critical infection surveillance. Furthermore, most phenotypic methods have been developed for individual bacterial taxa and are not transferable to other taxa without considerable adaptation.Hence, over the past 2 decades, phenotyping has been largely replaced by "molecular" genotyping. Clonal reproduction by binary fission imprints the evolutionary history of the organism in genotypic markers amenable to analysis by nucleic acid-mediated methods. However, in practice, the ease with which recombination, transfection, and transformation can take place in bacteria necessitates that data from multiple genetic markers are analyzed in defining a "precise" genotype and even then there is no guarantee that an adequate natural taxonomy will be derived. Polyphasic taxonomy currently uses combinations of different phenotypic and/or genotypic data sets to define genera, species, and entities at or below the subspecies level. Still, all these approaches remain time-consuming and relatively...

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Section: Typing By Maldi-tof Ms: What Can We Learn From Pfge?mentioning

confidence: 99%

Microbial Typing by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Do We Need Guidance for Data Interpretation?

et al. 2015

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“…However, infections other than cholera can be caused by nonepidemic V. cholerae serogroups that are collectively referred to as non-O1/non-O139 V. cholerae and are generally acquired through the consumption of raw or undercooked seafood. Non-O1/non-O139 V. cholerae infections are continuously reported worldwide (3,4), emphasizing their clinical significance. Although non-O1/non-O139 V. cholerae strains generally do not produce cholera toxin, other virulence factors contribute to their pathogenicity, including the hemolysin gene hlyA (5), the protease gene hapA (6), the cytotoxic actin cross-linking repeats in toxin gene rtxA (7), the sialidase gene nanH (8), the heat-stable toxin (NAG-ST) (9), a type 6 secretion system (T6SS) (10), and a type 3 secretion system (T3SS) (11).…”

mentioning

confidence: 99%

Non-O1/Non-O139 Vibrio cholerae Carrying Multiple Virulence Factors and V. cholerae O1 in the Chesapeake Bay, Maryland

Ceccarelli

Chen

Hasan

et al. 2015

Appl Environ Microbiol

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f Non-O1/non-O139 Vibrio cholerae inhabits estuarine and coastal waters globally, but its clinical significance has not been sufficiently investigated, despite the fact that it has been associated with septicemia and gastroenteritis. The emergence of virulent non-O1/non-O139 V. cholerae is consistent with the recognition of new pathogenic variants worldwide. Oyster, sediment, and water samples were collected during a vibrio surveillance program carried out from 2009 to 2012 in the Chesapeake Bay, Maryland. V. cholerae O1 was detected by a direct fluorescent-antibody (DFA) assay but was not successfully cultured, whereas 395 isolates of non-O1/non-O139 V. cholerae were confirmed by multiplex PCR and serology. Only a few of the non-O1/non-O139 V. cholerae isolates were resistant to ampicillin and/or penicillin. Most of the isolates were sensitive to all antibiotics tested, and 77 to 90% carried the El Tor variant hemolysin gene hlyA ET , the actin cross-linking repeats in toxin gene rtxA, the hemagglutinin protease gene hap, and the type 6 secretion system. About 19 to 21% of the isolates carried the neuraminidase-encoding gene nanH and/or the heat-stable toxin (NAG-ST), and only 5% contained a type 3 secretion system. None of the non-O1/non-O139 V. cholerae isolates contained Vibrio pathogenicity island-associated genes. However, ctxA, ace, or zot was present in nine isolates. Fifty-five different genotypes showed up to 12 virulence factors, independent of the source of isolation, and represent the first report of both antibiotic susceptibility and virulence associated with non-O1/non-O139 V. cholerae from the Chesapeake Bay. Since these results confirm the presence of potentially pathogenic non-O1/non-O139 V. cholerae, monitoring for total V. cholerae, regardless of serotype, should be done within the context of public health. Vibrio cholerae, a waterborne bacterial pathogen, is an autochthonous inhabitant of riverine and estuarine aquatic environments. There are Ͼ200 serogroups, based on O antigenic characters, but only serogroups O1 and O139 have been associated with epidemic cholera, and both are considered a major public health threat for developing countries (1).Developed countries today rarely witness cholera cases caused by epidemic strains of V. cholerae, and outbreaks are typically travel associated (2). However, infections other than cholera can be caused by nonepidemic V. cholerae serogroups that are collectively referred to as non-O1/non-O139 V. cholerae and are generally acquired through the consumption of raw or undercooked seafood. Non-O1/non-O139 V. cholerae infections are continuously reported worldwide (3, 4), emphasizing their clinical significance. Although non-O1/non-O139 V. cholerae strains generally do not produce cholera toxin, other virulence factors contribute to their pathogenicity, including the hemolysin gene hlyA (5), the protease gene hapA (6), the cytotoxic actin cross-linking repeats in toxin gene rtxA (7), the sialidase gene nanH (8), the heat-stable toxin (NAG-ST) (9), a type 6...

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“…Furthermore, Shiga toxin E. coli, Salmonella serotypes, C. difficile ribotypes and Vibrio cholera phenotypes have also been identified [51][52][53][54][55].…”

Section: Species Identification In 3 H Culturementioning

confidence: 99%

Recent Technology of Clinical Microbiology; Whole Genome Sequencing (WGS) and Matrix-Assisted, Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)

Jeongsook¹

2017

J Microb Biochem Technol

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Genetic and phenotypic analysis of Vibrio cholerae non-O1, non-O139 isolated from German and Austrian patients

Cited by 62 publications

References 42 publications

Microbial Typing by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Do We Need Guidance for Data Interpretation?

Microbial Typing by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Do We Need Guidance for Data Interpretation?

Non-O1/Non-O139 Vibrio cholerae Carrying Multiple Virulence Factors and V. cholerae O1 in the Chesapeake Bay, Maryland

Recent Technology of Clinical Microbiology; Whole Genome Sequencing (WGS) and Matrix-Assisted, Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)

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