Genetic and non-genetic parameters related to embryo production in superovulated Large White (LW) gilts

Furstoss, V.; Berthelot, F.; Plat, Martine; Venturi, Éric; Royer, Eric; Elleboudt, F.; Martinat-Botté, F.

doi:10.1016/j.anireprosci.2012.08.019

Cited by 6 publications

(9 citation statements)

References 33 publications

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“…These results are in agreement with our previous studies [6] and indicate that the superovulation treatment did not interfere with the events that control oocyte maturation, fertilization and early embryo development. It is generally assumed that superovulation treatments induce variability in the ovulatory response [20]. However, the ovulatory response variability found in the present study (CV<18%) and in our previous studies (CV = 21.4%) was much lower than previously reported values of approximately 40% in experiments with similar superovulation treatments (800 IU-1500 IU eCG) in cyclic gilts and sows [20]–[23].…”

Section: Discussioncontrasting

confidence: 87%

“…It is generally assumed that superovulation treatments induce variability in the ovulatory response [20]. However, the ovulatory response variability found in the present study (CV<18%) and in our previous studies (CV = 21.4%) was much lower than previously reported values of approximately 40% in experiments with similar superovulation treatments (800 IU-1500 IU eCG) in cyclic gilts and sows [20]–[23]. Although the reasons for these differences are unclear, these studies used different lines and breeds, which can exhibit widely variable superovulatory responses [7], possibly explaining such discrepancies.…”

Section: Discussionmentioning

confidence: 99%

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Successful Non-Surgical Deep Uterine Transfer of Porcine Morulae after 24 Hour Culture in a Chemically Defined Medium

et al. 2014

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Excellent fertility and prolificacy have been reported after non-surgical deep uterine transfers of fresh in vivo-derived porcine embryos. Unfortunately, when this technology is used with vitrified embryos, the reproductive performance of recipients is low. For this reason and because the embryos must be stored until they are transferred to the recipient farms, we evaluated the potential application of non-surgical deep uterine transfers with in vivo-derived morulae cultured for 24 h in liquid stage. In Experiment 1, two temperatures (25°C and 37°C) and two media (one fully defined and one semi-defined) were assessed. Morulae cultured in culture medium supplemented with bovine serum albumin and fetal calf serum at 38.5°C in 5% CO2 in air were used as controls. Irrespective of medium, the embryo viability after 24 h of culture was negatively affected (P<0.05) at 25°C but not at 37°C compared with the controls. Embryo development was delayed in all experimental groups compared with the control group (P<0.001). Most of the embryos (95.7%) cultured at 37°C achieved the full or expanded blastocyst stage, and unlike the controls, none of them hatched at the end of culture. In Experiment 2, 785 morulae were cultured in the defined medium at 37°C for 24 h, and the resulting blastocysts were transferred to the recipients (n = 24). Uncultured embryos collected at the blastocyst stage (n = 750) were directly transferred to the recipients and used as controls (n = 25). No differences in farrowing rates (91.7% and 92.0%) or litter sizes (9.0±0.6 and 9.4±0.8) were observed between the groups. This study demonstrated, for the first time, that high reproductive performance can be achieved after non-surgical deep uterine transfers with short-term cultured morulae in a defined medium, which opens new possibilities for the sanitary, safe national and international trade of porcine embryos and the commercial use of embryo transfer in pigs.

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Section: Discussioncontrasting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Successful Non-Surgical Deep Uterine Transfer of Porcine Morulae after 24 Hour Culture in a Chemically Defined Medium

et al. 2014

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“…This finding was not abnormal because such variability has been widely reported [39,40,41,42]. However, the variation observed in this study and our previous study [23] was low compared with those reported for cyclic gilts and sows, wherein the CVs were approximately 40% [39,40,41,42]. The different superovulation protocols and the different lines and breeds used among these studies, which can exhibit widely variable superovulatory responses [21], likely contributed to this discrepancy.…”

Section: Discussionsupporting

confidence: 60%

An Earlier Uterine Environment Favors the <i>In Vivo</i> Development of Fresh Pig Morulae and Blastocysts Transferred by a Nonsurgical Deep-uterine Method

Ángel

Gil

Cuello

et al. 2014

Journal of Reproduction and Development

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This study aimed to evaluate the effect of recipient-donor estrous cycle synchrony on recipient reproductive performance after nonsurgical deep-uterine (NsDU) embryo transfer (ET). The transfers (N=132) were conducted in recipients sows that started estrus 24 h before (–24 h; N=9) or 0 h (synchronous; N=31), 24 h (+24 h; N=74) or 48 h (+48 h; N=18) after the donors. A total of 30 day 5 morulae or day 6 blastocysts (day 0=onset of estrus) were transferred per recipient. The highest farrowing rates (FRs) were achieved when estrus appeared in recipients 24 h later than that in the donors (81.1%), regardless of the embryonic stage used for the transfers. The FR notably decreased (P<0.05) when recipients were –24 h asynchronous (0%), synchronous (61.3%) or +48 h asynchronous (50%) relative to the donors. No differences in litter size (LS) and piglet birth weights were observed among the synchronous and +24 h or +48 h asynchronous groups. While a +24 h asynchronous recipient was suitable for transfers performed with either morulae (FR, 74.3%; LS, 9.2 ± 0.6 piglets) or blastocysts (FR, 84.6%; LS, 9.8 ± 0.6 piglets), a + 48 h asynchronous recipient was adequate for blastocysts (FR, 87.5%; LS, 10.4 ± 0.7 piglets) but not for morulae (FR, 30.0%; LS, 7.3 ± 2.3 piglets). In conclusion, this study confirms the effectiveness of the NsDU-ET technology and shows that porcine embryos tolerate better a less advanced uterine environment if they are nonsurgically transferred deep into the uterine horn.

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“…; Furstoss et al. ) have reported CV values close to 40%. However, in the present study, the variability in the ovulatory response (CV = 19.4%) was similar to that reported for non‐superovulated gilts and sows (Longenecker and Day ; Gama and Johnson ; Bracken et al.…”

Section: Discussionmentioning

confidence: 89%

“…One problem related to superovulation treatment is the variability in the ovulatory response (Furstoss et al. ). Several experiments using superovulation treatments of 800 IU to 1500 IU eCG in cyclic gilts and sows (Hazeleger et al.…”

Section: Discussionmentioning

confidence: 99%

The Recipients' Parity Does Not Influence Their Reproductive Performance Following Non‐Surgical Deep Uterine Porcine Embryo Transfer

Martínez

Nohalez

Martínez

et al. 2015

Reprod Domestic Animals

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With the development of the non-surgical deep uterine (NsDU) embryo transfer (ET) technology, the commercial applicability of ET in pigs is now possible. There are, nevertheless, many factors that influence NsDU-ET effectiveness that need to be addressed. The aim of this study was to evaluate the effects of the weaned recipients' parity on fertility and prolificacy following NsDU-ET. The recipients (n = 120) were selected based on their reproductive history and body condition and grouped into three categories according to their parity: primiparous sows, sows of parity 2 and sows of parities from 3 to 5. Thirty fresh embryos (morulae and unhatched blastocysts) were non-surgically transferred into one uterine horn of each recipient. It was possible to insert the NsDU-ET catheter through the cervix along a uterine horn in 98.3% of the recipients. The parity had no influence on the difficulty grade of the insertions or on the percentage of correct insertions. The cervix and uterine wall were not perforated during the insertions, and vaginal discharge was not observed after transfer in any of the recipients. There were no differences in the pregnancy rates (74.8%), farrowing rates (71.2%) or litter sizes (9.6 ± 3.3) between groups. Also, there were no differences between groups regarding to the piglets' birthweights or piglet production efficiency. In conclusion, these results demonstrate that weaned sows from parity 1 to 5 are appropriate to be used as recipients in NsDU-ET programs, which increase the possibilities for the utilization of ET in the recipient farms.

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Genetic and non-genetic parameters related to embryo production in superovulated Large White (LW) gilts

Cited by 6 publications

References 33 publications

Successful Non-Surgical Deep Uterine Transfer of Porcine Morulae after 24 Hour Culture in a Chemically Defined Medium

Successful Non-Surgical Deep Uterine Transfer of Porcine Morulae after 24 Hour Culture in a Chemically Defined Medium

An Earlier Uterine Environment Favors the <i>In Vivo</i> Development of Fresh Pig Morulae and Blastocysts Transferred by a Nonsurgical Deep-uterine Method

The Recipients' Parity Does Not Influence Their Reproductive Performance Following Non‐Surgical Deep Uterine Porcine Embryo Transfer

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